Method for preparing ribostamycin rough products by using membrane separation technology

A ribosomycin and membrane separation technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of loss of ion exchange resin, difficult filtration of fermentation liquid, unshaped filter cake, etc. Emissions, simple process, short working time effect

Inactive Publication Date: 2012-07-04
福建和泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The shortcomings of the current technology are: dynamic adsorption process, even if filter aid is added in the fermentation liquid plate and frame filtration process, the fermentation liquid is still difficult to filter, the filtration speed is slow, the filtration pressure is as high as 0.4-0.6Mpa, the filter cake is not formed, and the feed liquid In the static adsorption process, the ion exchange resin is separated from the residue in the fermentation broth, the loss of the ion exchange resin is serious, the yield is low, and the wastewater pollution is serious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Using 260L of fermented broth with a ribomycin concentration of 5.5g / L as raw material, a ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 35L / h·㎡, the operating pressure is 2.5bar, and the operating temperature is 35°C. Under operating conditions, bacteria and residues in the fermentation broth are removed. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h m2, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove proteins, Polysaccharides and color-forming substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of a nanofiltration membrane flux of 30 L / h m2, an operating pressure of 10.0 bar, and an operating tempera...

Embodiment 2

[0022] Using 350L of fermented broth with a ribomycin concentration of 5.0g / L as raw material, ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 40L / h·㎡, the operating pressure is 3.0bar, and the operating temperature is 30°C. Under operating conditions, bacteria and residues in the fermentation broth are removed. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h m2, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove proteins, Polysaccharides and color-forming substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of the nanofiltration membrane flux of 30 L / h·㎡, the operating pressure of 8.0 bar, and the operating tempera...

Embodiment 3

[0024] Using 35L of powder mother liquor with ribomycin concentration of 98.0g / L as raw material, diluted with purified water to 350L, ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used, the flux is 40L h·㎡, and the operating pressure is 3.0bar. The impurities in the mother liquor are removed under the operating condition of an operating temperature of 30°C. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h·㎡, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove color-forming substances. Wait. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of the nanofiltration membrane flux of 30 L / h·㎡, the operating pressure of 10.0 bar, and the operating tempe...

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Abstract

The invention provides a method for preparing ribostamycin rough products by using membrane separation technology. The method comprises the steps of micro filter membrane edulcoration of feed liquid, ultrafiltration decoloration and nanofiltration desalination, enrichment, concentration and drying in a preparation process of the ribostamycin rough products. The feed liquid containing ribostamycin is carried out liquid and solid separation of micro filter membrane, protein micro filter permeating liquid, coloring matters, polysaccharide and other macromolecule impurities are removed through the ultrafiltration membrane, collected permeating liquid is desalted and enriched under normal temperature through the permeating liquid, and the desalt liquid is concentrated and dried to manufacture the ribostamycin rough products. The method for preparing ribostamycin rough products by using the membrane separation technology is novel in conception, mild in productive technology conditions, environment-friendly and clean, simple and easy to do, short in production cycle, low in production cost, high in extraction yield and strong in application, and has big application and dissemination values.

Description

Technical field [0001] The present invention involves a separation method of amino glycoside antibiotic nucleotoma, which is specifically involved in a method of using membrane separation technology to separate the production of ribromycin. Background technique [0002] Nurtuanycin is a broad -spectrum antibiotic that is effective for bacteria, splin bacteria, pneumococcus, pneumococcus, E. coli, and part of the transformer strain.Clinically applicable to sensitive intestinal bacteria such as Escharus, genus Crabobacteria, Transformers, Antiobacteria, etc.The respiratory tract, abdominal cavity, chest cavity, urinary tract, skin and soft tissue, bone tissue and eye, ear, and nose infection caused by the respiratory cavity, chest cavity, thoracic cavity, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, urinary tract, and soft tissue.Comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/23C07H1/08
Inventor 陈灼湖杨斌
Owner 福建和泉生物科技有限公司
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