Microbial fuel cell three-level continuous type waste water nitrogen-removing treatment method and device

A fuel cell and wastewater treatment technology, applied in biochemical fuel cells, biological water/sewage treatment, fuel cells, etc., can solve the problems of few microorganisms, complex species, unclear substrate pathways, etc., and achieve good treatment effect and treatment time. The effect of large and shortened start-up time

Active Publication Date: 2013-12-04
杭州唯铂莱生物科技有限公司
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  • Application Information

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Problems solved by technology

At present, there are still many problems in the research of microbial fuel cells in the treatment of wastewater: (1) the inoculum microorganisms are activated sludge, and the types of microorganisms in activated sludge are complex, metabolic pathways, substrate degradation mechanisms and electron transfer (2) The anode is made of carbon materials, and the enrichment of microorganisms on the surface of the anode is less, and its source mainly depends on the active adsorption of microorganisms themselves, so the power generation capacity of wastewater treatment microbial fuel cells is extremely low; (3) ) The nitrogen content in urban wastewater is relatively high, and the currently developed wastewater treatment microbial fuel cells can only degrade COD in wastewater, but cannot denitrify wastewater; (4) Most of the currently developed wastewater treatment microbial fuel cell devices are intermittent There are few studies on microbial fuel cell devices capable of continuous wastewater treatment, etc.

Method used

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  • Microbial fuel cell three-level continuous type waste water nitrogen-removing treatment method and device
  • Microbial fuel cell three-level continuous type waste water nitrogen-removing treatment method and device
  • Microbial fuel cell three-level continuous type waste water nitrogen-removing treatment method and device

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Embodiment 1

[0032] Embodiment 1: the preparation of novel anode

[0033] a. Production and pretreatment of anode plates: take a piece of square carbon fiber felt with a specification of 16×16cm and a thickness of 0.5cm, and take 16 pieces of strip-shaped carbon fiber felt with a specification of 16cm×1.0cm and a thickness of 0.5cm. Each of the 8 strip-shaped carbon fiber felts has a spacing of 1 cm, and is symmetrically fixed on both sides of the square carbon fiber felt to form a carbon fiber felt pole plate, and the carbon fiber felt pole plate is put into deionized water and boiled for 30 minutes. , and the carbon fiber felt anode plate prepared by natural cooling was stored in a refrigerator at 4°C for later use;

[0034] b. Preparation of Pseudomonas putida culture solution: Pseudomonas putida, the bacterial strain numbered as CGMCC NO: 1.1003 preserved on the slant, was inoculated into 50 mL of seed culture medium, and each of the seed culture medium was The dosage of components ar...

Embodiment 2

[0036] Embodiment 2: the preparation of novel anode

[0037] A. the making of anode plate and pretreatment are basically the same as embodiment 1, and difference is that the thickness of square carbon fiber felt is 0.8cm;

[0038] B. the preparation of Pseudomonas putida nutrient solution is identical with embodiment 1;

[0039] c. Immobilization of Pseudomonas putida: take the Pseudomonas putida culture solution obtained in b. step for subsequent use, take the carbon fiber felt anode plate obtained in a. step for subsequent use, and mix 10g of polyvinyl alcohol and 0.6g of alginic acid Add sodium to 100mL of distilled water, heat it in a boiling water bath to dissolve it, stir it evenly with a glass rod, cool to room temperature, mix it with 1.2g activated carbon powder, add 10mL of Pseudomonas putida culture solution, and mix it evenly , and then evenly spread the mixed solution on the carbon felt anode, and finally put the anode plate into the saturated boric acid solution...

Embodiment 3

[0040] Embodiment 3: the preparation of novel anode

[0041] A. the making of anode plate and pretreatment are basically the same as embodiment 1, the difference is that the thickness of the square carbon fiber felt is 1.0cm;

[0042] B. the preparation of Pseudomonas putida nutrient solution is identical with embodiment 1;

[0043] c. Immobilization of Pseudomonas putida: take the Pseudomonas putida culture solution obtained in step b. for subsequent use, and take the carbon fiber felt anode plate obtained in step a. for subsequent use, and mix 11g of polyvinyl alcohol and 0.7g of alginic acid Add sodium to 120mL of distilled water, heat it in a boiling water bath to dissolve it, stir it evenly with a glass rod, cool to room temperature, mix it with 1.3g of activated carbon powder, add 12mL of Pseudomonas putida culture solution, and mix it evenly , and then evenly spread the mixed solution on the carbon felt anode, and finally put the anode plate into the saturated boric ac...

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Abstract

The invention relates to a microbial fuel cell three-level continuous type waste water nitrogen-removing treatment method and a microbial fuel cell three-level continuous type waste water nitrogen-removing treatment device. The method is characterized by comprising the following steps: immobilizing the immobilized pseudomonas putida novel anode, which is obtained through manufacturing and pretreatment of an anode plate and immobilization of pseudomonas putida, in an anode chamber for treating ammonia nitrogen waste water; immobilizing the manufactured and pretreated cathode plate into a cathode chamber for treating nitrate nitrogen waste water, wherein the flow velocity is 0.4 to 0.8 mL / min; continuously treating at the temperature of 15 and 35 DEG C for 15 days; monitoring the output voltage of a microbial fuel cell on time through an electrochemical work station; and measuring the change of the ammonia nitrogen content of the ammonia nitrogen waste water and the change of the nitrate nitrogen content of the nitrate nitrogen waste water every other 24 hours, wherein the output voltage of the cell always ranges from 1.583 v to 1.867 v, the ammonia nitrogen removal rate of the nitrate nitrogen waste water always ranges from 55.6 to 64.7 percent, and the nitrate nitrogen removal rate of the nitrate nitrogen waste water always ranges from 84.6 to 92.3 percent. The pseudomonas putida is derived from the China General Microbiological Culture Collection Center.

Description

technical field [0001] The invention relates to the field of microbial fuel cells, in particular to a microbial fuel cell integrating power generation and continuous denitrification of domestic wastewater. Background technique [0002] In today's world, reducing pollutant emissions and dependence on fossil fuels are two goals that sustainable development strives to achieve. Microbial fuel cell technology offers the potential to achieve both goals in wastewater treatment and power generation. [0003] Microbial fuel cell is a special kind of fuel cell. It is a device that uses microorganisms as a catalyst to directly convert the chemical energy of organic matter into electrical energy. Experimental studies have shown that microbial fuel cells can not only use acetate and other substances as fuels to continuously and stably generate current, but also use various waste water and organic pollutants as fuels. At present, there are still many problems in the research of microbi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01M8/16C02F3/34H01M8/06H01M8/04
CPCY02E60/50Y02E60/527Y02P70/50
Inventor 于大禹王刚乔楠徐富超易景王钦强邢兆强杨晓航高林牛方明
Owner 杭州唯铂莱生物科技有限公司
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