Bacillus atrophaeus, and preparation and application of bacillus atrophaeus

A technology of Bacillus atrophaeus and microbial preparations, applied in the field of aquatic application microorganisms, can solve the problems of lagging disease control technology, restricting the healthy and sustainable development of the industry, and economic losses, etc., and achieve the effects of fast reproduction, ensuring food safety, and reducing drug residues

Inactive Publication Date: 2012-07-11
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the high-density breeding and non-standard operation of sea cucumbers and the relative lag of disease

Method used

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  • Bacillus atrophaeus, and preparation and application of bacillus atrophaeus
  • Bacillus atrophaeus, and preparation and application of bacillus atrophaeus
  • Bacillus atrophaeus, and preparation and application of bacillus atrophaeus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Screening and isolation process of Aj080319IA-12 strain:

[0043] The Aj080319IA-12 strain was isolated from the intestines of wild sea cucumbers by the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences. In 2008, the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences captured wild sea cucumbers from the sea area of ​​Taipingjiao, Qingdao. Under sterile conditions, the sea cucumbers were live dissected. The intestinal tract was rinsed three times with 15‰ normal saline, and the washed intestinal homogenate was uniformly diluted and spread on TSB medium and 2216E seawater agar medium, and the plates with appropriate dilution were selected for bacterial isolation and passed multiple Bacteria were cultured and purified by streaking, and the morphological structure and quantity of the bacteria were recorded and photographed; the common pathogenic bacteria of A. japonicus - Vibrio splendidus (Vibrio splendidus),...

Embodiment 2

[0045] Morphological characteristics of Aj080319IA-12 strain

[0046] Gram staining of the cells of the strain Aj080319IA-12 was positive, the cells were rod-shaped, spore staining showed spores, and capsule staining showed that the cells had no capsule. On the TSB solid plate medium, the colony is yellow, the surface is wrinkled, silky, opaque, the edges are irregular, and raised. After 48 hours of culture, the colony is black around the colony, and there is a bacterial film and precipitates during liquid culture.

Embodiment 3

[0048] 16S rDNA sequence determination

[0049] Such as figure 1 Shown is the results of the phylogenetic tree obtained by using the PHYLIP software according to the 16S rDNA sequence described in the embodiment of the present invention.

[0050] The universal primers used to amplify bacterial 16S rDNA were 27F: 5’-AGAGTTTGATC(C / A)TGGCTCAG-3’ and reverse primer 1492R: 5’-TACGG(C / T)TACCTTGTTACGACTT-3’. The 16S rDNA was amplified using the genomic DNA of the Aj080319IA-12 strain as a template and transformed and sequenced. See the sequence table for the obtained sequence. The obtained 16S rDNA sequence of the Aj080319IA-12 strain was compared in Genbank for homology, and Clustalw software was used for multiple sequence matching alignment, and the phylogenetic inference software package PHYLIP4.0 was used for statistical analysis and clustering analysis, and the neighbor linking method was used Obtain a phylogenetic tree ( figure 1 ), the phylogenetic tree was evaluated by boo...

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Abstract

The invention discloses a bacillus atrophaeus strain Aj080319IA-12. The bacillus atrophaeus strain is characterized by being separated from an intestinal tract of a wild stichopus japonicus selenka and collected in China Center for Type Culture Collection (CCTCC) along with a collection number of CCTCC M 2010316. The screened bacillus atrophaeus has remarkable antagonistic action on pathogenic bacteria of a sea cucumber, and has the effects of increasing the growth speed, immunity and intestinal digestive enzyme activity of the stichopus japonicus selenka. The screened bacillus atrophaeus is indigenous bacterium screened from the intestinal tract of the sea cucumber, which is safe and non-toxic for the sea cucumber, fast in breeding speed, easy to culture and strong in adaptability on environment, and is capable of being bred quickly to form predominant bacteria. A microbiological preparation, as a feed additive, can increase the specific growth rate of the sea cucumber, reduce the death rate of the sea cucumber, improve the immunity of the sea cucumber and the digestive enzyme activity of the intestinal tract, and improve the disease resistance of the sea cucumber at the same time, so that the preparation achieves the effects of resisting bacteria, immunizing and promoting growth, and is capable of reducing and replacing the use of antibiotics.

Description

technical field [0001] The invention relates to the technical field of aquatic application microorganisms, in particular to a bacillus atrophaeus and the production of microbial preparations using the bacillus atrophaeus as well as its preparation method and application. Background technique [0002] Intestinal probiotics are a live bacterial preparation that mostly originates from the digestive tract and acts on the digestive tract. It can effectively supplement the beneficial microorganisms in the digestive tract of animals, inhibit the growth of pathogenic bacteria, improve the balance of digestive tract flora, and improve the immune function of aquatic animals. or growth rate. [0003] Sea cucumber (Apostichopus japonicus Liao, also known as sea cucumber), belongs to invertebrates, Echinodermata, sea cucumber class, and sea cucumber genus. Because of its high nutritional value, the scale of cultivation continues to expand, and it has developed into one of the largest ma...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/07
Inventor 廖梅杰李彬王印庚王崇明张正荣小军陈贵平王岚陈霞曲江波
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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