Method for improving strength and concentration of butane diacid generated from anaerobic fermentation
A technology of succinic acid production and anaerobic fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effect of increasing cell membrane channels, increasing concentration, and accelerating growth
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Embodiment 1
[0016] Bacteria: Actinobacillus succinogenes Actinobacillus succinogenes NJ113 (CGMCC NO.1716) This bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C.
[0017] The fermentation conditions are as follows:
[0018] Activation of strains: After the strains were streaked on the slant medium, they were activated and cultured in an anaerobic incubator at 37°C for 24 h. Seed culture: transfer the activated cultured strains to the seed medium, and cultivate them at 37°C for 10-12 hours as the seed liquid.
[0019] Fermentation of succinic acid: Put the activated seed solution and sterilized glucose into the fermenter, the inoculation amount is 5% (v / v), the stirring speed is 200 rpm, and the fermentation is carried out at 37°C, CO 2 The ventilation volume is 0.25 vvm; the timing starts at the beginning of this step, and at the 6th hour, a concentration of 0-5 g / L Tween-80 is added to the fermentation syst...
Embodiment 2
[0027] Strain activation and slant medium, seed medium and seed culture method are the same as in Example 1.
[0028] Fermentation of succinic acid: Put the activated seed solution and the sterilized glucose into the fermenter, the inoculation amount is 10% (v / v), the stirring speed is 200 rpm, and the fermentation is carried out at 37°C, CO 2 The ventilation volume is 0.25 vvm; the timing starts at the beginning of this step, and at the 7th hour, a concentration of 0-5 g / L OP-10 is added to the fermentation system; the total culture time is 15 hours, and the pH is controlled at 7 throughout the fermentation process.
[0029] Fermentation medium (g / L): OP-10 0~5, glucose 30 (based on total reducing sugar concentration) and separately sterilized and added separately, yeast powder 10, sodium acetate 1.36, KH 2 PO 4 3. MgCl 2 ·6H 2 O 0.2, CaCl 2 0.2, NaCl 1, NaH 2 PO 4 2H 2 O 0.31, NaH 2 PO 4 2H 2 O 1.6, corn steep liquor dry powder 7.5, pH 7.0, sterilized at 121°C ...
Embodiment 3
[0034] Strain activation and slant medium, seed medium and seed culture method are the same as in Example 1.
[0035] Fermentation to produce succinic acid: put the activated seed solution and sterilized glucose into the fermenter, the inoculum amount is 3 % (v / v), the stirring speed is 200 rpm, and the fermentation is carried out at 37°C, CO 2 The aeration volume is 0.25 vvm; the timing starts at the beginning of this step, and at the 6.6th hour, a concentration of 0-5 g / L SDS is added to the fermentation system; the total incubation time is 15 hours, and the pH is controlled at 6.8 throughout the fermentation process.
[0036]Fermentation medium (g / L): SDS 0-5, glucose 30-60 (based on total reducing sugar concentration) and added separately for sterilization, yeast powder 10, sodium acetate 1.36, KH 2 PO 4 3. MgCl 2 ·6H 2 O 0.2, CaCl 2 0.2, NaCl 1, NaH 2 PO 4 2H 2 o 0.31, NaH 2 PO 4 2H 2 O 1.6, corn steep liquor dry powder 7.5, pH 7.0, sterilized at 121°C for...
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