Preparation method and application of tea polyphenol chitosan nanoparticles

Abstract

The invention relates to a preparation method and application of tea polyphenol chitosan nanoparticles, in particular to novel tea polyphenol nanoparticles. The nanoparticles are prepared from the following components in parts by weight: 3 to 6 parts of chitosan, 1 to 2 parts of porous silicon dioxide and 1 to 4 parts of tea polyphenol. The porous silicon dioxide is added into the tea polyphenol nanoparticles, so that the tea polyphenol content of the nanoparticles can be remarkably increased. The tea polyphenol chitosan nanoparticles are prepared by a sodium tripolyphosphate (TPP) ionic gel method. The nanoparticles can be widely applied to various fields of biomedicine and health care products, and can be used for lowering blood cholesterol, treating obesity, resisting oxidation, clearing free radicals and preventing and treating tumors.

Description

technical field [0001] The invention relates to a nano particle, in particular to a tea polyphenol chitosan nano particle and a preparation method and application thereof. Background technique [0002] Chitosan (Chitosan, CS) is a natural polysaccharide chitin, CS is also called chitosamine, chitosan, etc., and is the β-(1,4)-2-acetylamino-D- Copolymers of glucose units and β-(1,4)-2-amino-D-glucose units, the latter generally exceeding 80%. CS is the only cationic polysaccharide in nature, which is biodegradable, non-toxic, has good biocompatibility, high charge density and high adhesion to mucous membranes. In recent years, the research of CS and its derivatives in the fields of biomedicine, pharmacy, and bioengineering has become increasingly active. For example, the absorbable sutures and artificial skin made by it have entered clinical applications and are commercially available. In addition to the characteristics of chitosan, chitosan nanoparticles also have the gene...

AI Technical Summary

Problems solved by technology

②Covalent cross-linking method, that is, using water / oil emulsifier for emulsification, using glutaraldehyde as cross-linking agent to cross-link the free amino groups of chitosan, the particles obtained by this method are stable in shape and uniform in particle size, but a large amount of Organic solvents and surfactants, and glutaraldehyde has certain cytotoxicity and inactivation of macromolecular drugs

However, these ingredients are prone to oxidation, polymerization, condensation and other reactions under conditions such as humidity, sunlight, and high temperature. The stability is very poor, and the oral bioavailability is particularly low. In addition, there are problems such as bitter taste, which limits the use of tea Application of phenol (8-11)

It is also reported in the literature that chitosan nanoparticles are used to encapsulate tea polyphenols (12-13), but the research results show that the content of tea polyphenols in nanoparticles is relatively low, and the stability of tea polyphenols has not been fundamentally affected. Improvement, the bioavailability of tea polyphenols has not been greatly improved