Preparation method and application of tea polyphenol chitosan nanoparticles

A technology of chitosan nanoparticles and tea polyphenols, applied in the field of nanoparticles, can solve the problems of easy oxidation, polymerization, condensation, the bioavailability of tea polyphenols is not greatly improved, and the content is low.

Inactive Publication Date: 2012-08-15
无锡纳诺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

②Covalent cross-linking method, that is, using water/oil emulsifier for emulsification, using glutaraldehyde as cross-linking agent to cross-link the free amino groups of chitosan, the particles obtained by this method are stable in shape and uniform in particle size, but a large amount of Organic solvents and surfactants, and glutaraldehyde has certain cytotoxicity and inactivation of macromolecular drugs
However, these ingredients are prone to oxidation, polymerization, condensation and other reactions under conditions such as humidity, sunlight, and high tempe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] [Example 1] Preparation of tea polyphenol chitosan nanoparticles

[0022] Tea polyphenol chitosan nanoparticles were prepared by sodium tripolyphosphate (TPP) ion gel method.

[0023] Nanoparticle 1: First, 10 mg of chitosan (molecular weight: 200,000) was dissolved in 5 ml of 0.3% acetic acid aqueous solution by volume fraction, and fully swelled overnight. 100mg of porous silicon dioxide was dispersed in 5mg / ml tea polyphenol aqueous solution, and magnetically stirred for 2h. Mix the chitosan solution and the tea polyphenol solution containing silicon dioxide, under magnetic stirring at room temperature, slowly add 1 mg / ml TPP aqueous solution into the mixture, and continue stirring for 1 hour after the dropwise addition to obtain tea polyphenols Colloidal solution of phenolic nanoparticles. Take the nanoparticle colloidal solution and add lactose as a freeze-drying protective agent, and freeze-dry for 48 hours to obtain the tea polyphenol nanoparticle solid powder....

Embodiment 2

[0027] [Example 2] Stability investigation of tea polyphenol chitosan nanoparticles

[0028] After placing the tea polyphenol raw material, nanoparticle 1, and nanoparticle 2 at room temperature for 2 months, it was found that the tea polyphenol raw material absorbed moisture after being placed for a few days, and the color turned red. Scanning with ultraviolet wavelengths showed that the tea polyphenol was oxidized into Quinone substances form dimers, and the maximum absorption peak is red-shifted. However, in nanoparticles 1 and 2, the tea polyphenols were encapsulated in the microspheres, the color did not change significantly, and the maximum ultraviolet absorption wavelength did not change.

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Abstract

The invention relates to a preparation method and application of tea polyphenol chitosan nanoparticles, in particular to novel tea polyphenol nanoparticles. The nanoparticles are prepared from the following components in parts by weight: 3 to 6 parts of chitosan, 1 to 2 parts of porous silicon dioxide and 1 to 4 parts of tea polyphenol. The porous silicon dioxide is added into the tea polyphenol nanoparticles, so that the tea polyphenol content of the nanoparticles can be remarkably increased. The tea polyphenol chitosan nanoparticles are prepared by a sodium tripolyphosphate (TPP) ionic gel method. The nanoparticles can be widely applied to various fields of biomedicine and health care products, and can be used for lowering blood cholesterol, treating obesity, resisting oxidation, clearing free radicals and preventing and treating tumors.

Description

technical field [0001] The invention relates to a nano particle, in particular to a tea polyphenol chitosan nano particle and a preparation method and application thereof. Background technique [0002] Chitosan (Chitosan, CS) is a natural polysaccharide chitin, CS is also called chitosamine, chitosan, etc., and is the β-(1,4)-2-acetylamino-D- Copolymers of glucose units and β-(1,4)-2-amino-D-glucose units, the latter generally exceeding 80%. CS is the only cationic polysaccharide in nature, which is biodegradable, non-toxic, has good biocompatibility, high charge density and high adhesion to mucous membranes. In recent years, the research of CS and its derivatives in the fields of biomedicine, pharmacy, and bioengineering has become increasingly active. For example, the absorbable sutures and artificial skin made by it have entered clinical applications and are commercially available. In addition to the characteristics of chitosan, chitosan nanoparticles also have the gene...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61K47/36A61K9/14A61P3/06A61P35/00A61P29/00A61P39/00A61P39/06
Inventor 马会利李利鹏
Owner 无锡纳诺生物科技有限公司
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