Algal epiphytic fungus chlorinated depside cyclic ether compound, and preparation and application thereof
A technology of chlorinated depsipepsyl acid cyclic ether and daisylated phenolic acid cyclic ether, applied in the field of chlorinated depsipepsyl acid cyclic ether compounds of epiphytic seaweed fungi and their preparation and application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 3
[0050] Inhibition of Gram-positive drug-resistant bacteria activity test:
[0051] Staphylococcus aureus is a very common germ that sometimes enters the body and causes an infection. The infection can range from mild sores and pimples on the skin to pneumonia or blood infection. Infections caused by staph bacteria are usually treated with the penicillin-type antibiotic methicillin, which is very effective in most cases. However, some staphylococcus strains have developed resistance to methicillin, that is, methicillin-resistant Staphylococcus aureus (MRSA). The first case of MRSA was discovered in the United Kingdom in 1961, and then it spread around the world at an alarming rate. It is the most common Gram-positive drug-resistant bacteria in clinical practice. Important target pathogenic microorganisms for the development of new antibiotics.
[0052] Method: The international standard micro broth dilution method (National Committee for Clinical Laboratory Standards. 2003. ...
Embodiment 4
[0055] DNA damage repair experiments targeting Escherichia coli:
[0056] The mechanism of the DNA damage repair experiment is to use two strains of E. coli donated by the E. coli strain bank of Yale University in the United States to carry out the control antibacterial test. One strain is wild-type E. coli AB1157 with normal DNA damage repair genes, and the other strain is DNA damage repair genes. For defective Escherichia coli AB3027, if the test sample shows significant selective inhibition to the defective strain, it indicates that the sample may have a DNA damage mechanism. Since many antibacterial and antitumor drugs have a DNA damage mechanism, the control The test can be used to screen antibacterial and antitumor drug candidates.
[0057] Method: The chlorinated depsipeptide was determined by the international standard broth dilution method (National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria ...
Embodiment 5
[0060] Insecticidal activity test:
[0061] Artemia (brine shrimp), also known as saltwater Artemia, belongs to the phylum Arthropoda, Crustacea, Anocaria, Salina Artemia, Artemia genus, is a fish bait organism with high economic value, and is also a kind of For important experimental animals that are more sensitive to toxins, the 2nd to 3rd instar larvae cultured in the laboratory can be used to evaluate the activity of insecticides.
[0062] Insecticidal activity test method (see the applicant's paper Toxicology Mechanisms and Methods, 2012, 22(1), 23-30 for details).
[0063] Artemia hatching and collection: Frozen Artemia eggs that have been stimulated and revived by indoor natural light are added to the hatching tank of a double-chamber connector culture device for cultivation. The connector is filled with seawater (30g / L) prepared from natural coarse sea salt. Dosing amount is 1g / L, first close the channel of the connector. After hatching at 28°C for 24 hours under lig...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More