Strain of copper-resistant bacteria and application thereof

A technology of bacteria and copper pollution, applied in the field of microorganisms, can solve the problems of short plants, low biomass, and long growth cycle of hyperaccumulative plants, and achieve the effects of improving phytoremediation efficiency, increasing total absorption, and increasing biomass

Inactive Publication Date: 2012-09-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the deepening of research, it has been found that hyperaccumulator plants are often short in size, low in biomass, slow in growth rate, and long in g

Method used

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  • Strain of copper-resistant bacteria and application thereof
  • Strain of copper-resistant bacteria and application thereof
  • Strain of copper-resistant bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1.1 Strain isolation and purification

[0028] The soil samples were collected from the tailing vegetable garden soil of Funiushan Copper Mine in Tangshan Town, Nanjing City, the tailing soil of Anji Mountain, and the plant rhizosphere soil of the tailing soil of Fenghuang Mountain and Tongguan Mountain in Tongling. Within one week, the soil samples were separated and purified by dilution plate coating method (Shen Ping et al., 1999) and plate streaking method. Part of the soil samples were air-dried and stored for soil sample nutrient and heavy metal element analysis, mainly to determine the contents of water-soluble Cu, exchangeable Cu and total Cu in the soil. The specific steps of strain isolation and purification are as follows:

[0029] (1) Pour plate Prepare nitrogen medium, mix well, and pour plate after autoclaving. Nitrogenous medium formula is: sucrose 10g, (NH 4 ) 2 SO 4 1.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, yeast extract 0.5g, disti...

Embodiment 2

[0051] Activation of Cu by the bacterial strain DGS6 of embodiment 2

[0052] 2.1 Soil sample collection

[0053] The soil samples were collected from the polluted vegetable garden near the Funiushan Copper Mine in Tangshan Town, Nanjing City. After the soil samples were air-dried, stone particles and plant roots were removed, and passed through a 2mm sieve. For the analysis of soil sample nutrients and heavy metal elements, it mainly determines the water-soluble Cu and exchangeable Cu in the soil. Part of the samples were ground in an agate mortar and passed through a 100-mesh sieve for the determination of total Cu content.

[0054] 2.2 Activation test of the tested strains on Cu in soil

[0055] Put 3.00g of sterilized and non-sterilized test soil into 50mL centrifuge tubes respectively. After the test strain was cultured in a liquid nitrogen medium for 16 hours, take 3.0 mL of the DGS6 bacterial solution (CGMCC NO.4817) in the logarithmic growth phase and pour it into a...

Embodiment 3

[0060] Effect of embodiment 3 bacterial strains on plant growth and absorbing Cu

[0061] 3.1 Effects of strain DGS6 on the elongation of the roots of A. haizhou, corn and oil sunflower

[0062] Cultivate the strain DGS6 (CGMCC NO.4817) in a nitrogenous medium for 24 hours, centrifuge the pure culture at 4°C, discard the supernatant, wash twice with sterile water, resuspend in sterile water, and measure at 600nm Adjust the optical density of the bacterial suspension to 0.5±0.02. Soak the seeds of Azalea, corn, and sunflower in 70% alcohol for 1 minute, then disinfect with 1% sodium hypochlorite for 10 minutes, and wash them with sterile water for more than 5 times immediately. Soak the seeds in bacterial suspension or sterile water for 3 hours at room temperature, then place them on a filter paper wetted with sterile water, Cu solution or bacterial solution in a petri dish, and place them on a slight inclination (to prevent water flooding and seeds). Root length was measured...

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Abstract

The invention discloses a strain of copper-resistant bacteria and an application thereof. The copper-resistant bacteria DGS6 is preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO. 4817. The bacterial strain DSG6 not only can dissolve the insoluble copper carbonate in the liquid culture base, but also can activate the copper (Cu) in the soil. The bacterial strain can effectively promote the elongation growth of elsholtzia splendens nakai, corn and sunflower roots. A potted plant experiment shows that the bacterial strain DGS6 canpromote the growth of plants and enhance the biomass of the plants. Experimental results show that compared with a reference, after the bacterial strain DGS6 is inoculated, the dry weight of a floor part and the dry weight of the roots of the corn are respectively increased by 49 percent and 35 percent, the dry weight of the floor part and the dry weight of the root of the sunflower are respectively increased by 85 percent and 42 percent. The pseudomonas DGS6 with the preservation number of CGMCC No. 4817 can be applied in promoting the growth of the plants.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a copper-resistant bacterium and its application. Background technique [0002] At present, soil, as an important part of the ecological environment on which human beings depend, is increasingly polluted by heavy metals. The world's average annual emission of Hg is about 15,000 tons, Cu is 3.4 million tons, Pb is 5 million tons, Mn is 15 million tons, and Ni is 1 million tons. Heavy metal pollution in my country's soil is also quite serious. Millions of tons of toxic heavy metals are released into the environment every year, including 346,000 tons of Pb and 39,000 tons of Cd (Nriagu and Pacyna, 1988). [0003] The pollution of harmful metals in the soil directly affects soil quality, water quality, crop growth, agricultural output and quality, etc., and is enriched in humans and animals through the food chain, endangering the health of humans and animals, and causing human cancer and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00B09C1/10C12R1/38
Inventor 陈亚华杨仁秀沈振国夏妍王桂萍
Owner NANJING AGRICULTURAL UNIVERSITY
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