Sulforaphane microencapsulation method

Abstract

The invention discloses a sulforaphane microencapsulation method which comprises the following steps of: I, extraction of sulforaphane: performing digestion of the raw material crucifer to extract the sulforaphane, wherein the extraction concretely comprises the following steps of: A, grinding seeds; B, adding petroleum ether for degreasing; C, adopting phosphate buffer solution with pH value of 7.0; D, performing enzymolysis; E, adding an extractant for extraction; F, adding sodium sulfate and sodium chloride respectively, and filtering; G, performing vacuum concentration to remove the organic solvent; H, fixing the volume to 5,000ml with methanol; I, filtering with a 0.22-micron microfiltration membrane; and J, measuring the content of sulforaphane by liquid chromatography; and II, microencapsulation of sulforaphane: adding monoglyceride and sodium alginate into the composite wall material, adding the deionized aqueous solution, adding the sulforaphane while stirring, spray-drying and collecting the product. In the method disclosed by the invention, the extremely unstable sulforaphane liquid is synthesized into powdery solid particles through a microencapsulation method so as to obtain perfect stability.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical synthesis, in particular to a microcapsule embedding method of sulforaphane. Background technique [0002] Sulforaphane (1-isothiocyanate-4-methanesulfonylbutane, Sulforaphane), also known as radish, is an isothiocyanate derivative, easily soluble in water, with a relative molecular weight of 177.29 and molecular formula C 6 h 11 S 2 NO. It is produced by enzymatic hydrolysis (myrosinase) or acid hydrolysis of its precursor substance, glucosinolate (a type of glucosinolate). [0003] In recent years, the incidence of cancer has been on the rise, but the current medicine and medicine are difficult to achieve a radical cure, and can only focus on prevention; research has found that sulforaphane is considered to be the strongest antioxidant found in vegetables so far. One of the cancer components, a large number of in vitro tests have confirmed that sulforaphane has excellent anti-cancer function...

AI Technical Summary

Problems solved by technology

[0003] In recent years, the incidence of cancer has been on the rise, but the current medicine and medicine are difficult to achieve a radical cure, and can only focus on prevention; research has found that sulforaphane is considered to be the strongest antioxidant found in vegetables so far. One of the cancer components, a large number of in vitro tests have confirmed that sulforaphane has excellent anti-cancer functions, however, sulforaphane is a very unstable liquid, and there is currently no good way to stabilize it in China

[0004] In foreign countries, developed countries such as Japan and the United States have made some progress in the research on large-scale preparation of sulforaphane. The production processes that have been explored mainly include chemical synthesis and enzymatic methods. British scientists use natural hybridization methods to The original broccoli was hybridized with the Sicilian species to obtain a super cauliflower, in which the sulforaphane content was 100 times higher than the original, but it is still in its infancy, and there is no related report and patent application in our country, so for this natural, The research on substances with a strong anti-cancer effect still needs to be strengthened. With the deepening of research on sulforaphane, people urgently need a technical route that can make sulforaphane into food additives and even functional health products; but people The current reports on isothiocyanates such as sulforaphane are mainly in the aspects of analysis and detection, anti-cancer mechanism research, etc.

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