Method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials

A technology of mixed fermentation and bagasse, which is applied in the field of bioengineering to achieve high yield and conversion rate and reduce the cost of raw materials

Inactive Publication Date: 2012-10-03
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the application of cellulase enzymatic hydrolysis of bagasse technology in industrial production is not yet mature, but low-concentration mixed sugars can be obtained by dilute sulfuric acid hydr

Method used

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  • Method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials
  • Method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials
  • Method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials

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Experimental program
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Effect test

Embodiment 1

[0019] The method for producing butanol by mixed fermentation of raw materials with bagasse and molasses of the present invention comprises the steps:

[0020] Step 1, the preparation of bagasse hydrolyzate

[0021] The bagasse is dried at 60°C, crushed and passed through a 40-80 mesh sieve. Hydrolyze with 0.25-3 w / v % dilute sulfuric acid, wherein the solid-liquid ratio of bagasse to dilute sulfuric acid is 8 w / w %, the hydrolysis temperature is 121°C, and the hydrolysis time is 60 minutes. After hydrolysis, neutralize with sodium hydroxide. Then filter the polysaccharide hydrolyzate. DNS method was used to measure the total sugar, and liquid chromatography was used to detect the content of xylose and glucose in the hydrolyzed solution. The results of hydrolysis are shown in Table 1.

[0022] Table 1 Components and hydrolysis rate of hydrolyzate obtained by hydrolysis of 8% (material-to-water ratio) bagasse with different dilute acid concentrations at 121°C for 1 h

[0023...

Embodiment 2

[0034] Step 1 is the same as in Example 1. Plate culture medium and seed culture medium are the same as embodiment 1.

[0035] Step 2. Clostridium beijerinckii gxas.28 uses bagasse hydrolyzate and molasses as raw materials to produce butanol through mixed fermentation

[0036] Fermentation medium Ⅰ: 80 sag molasses 50g, yeast powder 5g, calcium carbonate 3.0g, superphosphate 0.7g, ammonium sulfate 3.0g, bagasse hydrolyzate prepared by 0.5% volume dilute sulfuric acid to 1000mL, pH 6.2~6.5 . Mix the above ingredients evenly, divide them into 250 mL Erlenmeyer flasks, fill each Erlenmeyer flask with 200 mL, seal with rubber stoppers, and autoclave at 115°C for 15 minutes.

[0037] Fermentation medium Ⅱ: 60g of 80% molasses, 5g of yeast powder, 3.0g of calcium carbonate, 0.7g of superphosphate, 3.0g of ammonium sulfate, dilute sulfuric acid with 0.5% volume of bagasse hydrolyzate to 1000mL, pH 6.2~6.5 . Mix the above ingredients evenly, divide them into 250 mL Erlenmeyer flas...

Embodiment 3

[0044] Plate culture medium and seed culture medium are the same as embodiment 1.

[0045] Clostridium beijerinckii (Clostridium beijerinckii) gxas.28 fermented butanol from glucose, xylose and mixtures.

[0046] Fermentation medium Ⅰ: glucose 60g, beef extract 2g, yeast powder 2g, peptone 6g, ammonium acetate 3g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01 g, agar 20 g, distilled water to 1000mL. Mix the above ingredients evenly, divide them into 250 mL Erlenmeyer flasks, fill each Erlenmeyer flask with 200 mL, seal with rubber stoppers, and autoclave at 115°C for 15 minutes.

[0047] Fermentation medium Ⅱ: xylose 60g, beef extract 2g, yeast powder 2g, peptone 6g, ammonium acetate 3g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01 g, agar 20 g, distilled water to 1000mL. Mix the above ingredients evenly, divide them into 250 mL Erlenmeyer flasks, fill each Erlenmeyer flask with 200 mL, seal with rubber stoppers, and autoclave at 115°C for...

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Abstract

The invention discloses a method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials. The method comprises the following steps of: hydrolyzing the crushed bagasse by using dilute acid, directly mixing the obtained hydrolysate and the molasses to form the fermentation raw materials, and fermenting the fermentation raw materials by using Clostridium beijerinckii gxas. 28CCTCC M201454 to produce the butanol. The yield of the butanol can reach 11.07 g/L. By adopting the method, the sugar making byproduct of sugarcane can be effectively used for producing the butanol through fermentation, the process is simple, the raw material cost for producing the butanol by microbial fermentation is reduced, and the method has social significance and application value.

Description

technical field [0001] The present invention relates to the production method of acetone and butanol. Specifically, it uses cheap bagasse and molasses as raw materials to convert pentose and hexose into acetone and butanol by Clostridium beijerinckii gxas.28, which belongs to biological field of engineering technology. Background technique [0002] Butanol is an important C4 platform compound, mainly used in the synthesis of n-butyl phthalate, butyl phosphate, butyl acrylate and butyl acetate; it can also be used as an extraction solvent for oils, medicines and spices and Alkyd resin additives and other fields. With the diversified development of biomass energy, butanol is considered to be one of the most potential new liquid fuels because of its high energy density, low hydrophilicity, low vapor pressure, and high octane number. [0003] Butanol fermentation was once the second largest fermented product in the world after ethanol fermentation. my country has established ...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12R1/145
Inventor 裴建新庞浩林丽华郭媛黎贞崇陈东黄志民黄日波
Owner GUANGXI ACAD OF SCI
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