Efficient purification technology for ganoderma lucidum polysaccharide
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A technology of Ganoderma lucidum polysaccharides and Ganoderma lucidum fruiting bodies, applied in the direction of membrane technology, chemical instruments and methods, ultrafiltration, etc., can solve the problems of unsuitability for large-scale industrial production, long concentration time, and low sample purity, and achieve short operation time, The effect of less solvent consumption and high purity of active ingredients
Active Publication Date: 2014-06-25
NANJING AGRICULTURAL UNIVERSITY +1
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Fractional precipitation can only be used for preliminary purification, and the purity of the prepared samples is low, while molecular sieve, electrophoresis, ion exchange chromatography, etc. generally have less processing capacity, long concentration time, high cost, and are not suitable for large-scale industrial production
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Embodiment 1
[0026] Example 1: Concentrating Ganoderma lucidum polysaccharide by vacuum rotary evaporation
[0027] ①Weigh 20 grams of Ganoderma lucidum fruiting body powder, add 560ML pure water and mix well, extract with an ultrasonic cell pulverizer (Zhejiang Ningbo Xinzhi Company; model: JY98-III ultrasonic cell pulverizer) at 680W for 17min, and remove the residue of Ganoderma lucidum by suction filtration;
[0028] ② Concentrate the filtrate to 140ML using a rotary evaporator;
[0029] ③ Slowly add 560ml of 95% ethanol to the concentrated solution while stirring, alcohol precipitation at 4°C for 12h, centrifuge at 4500rpm for 15min, discard the supernatant, wash the precipitate three times with absolute ethanol, acetone, ether, and freeze-dry Obtain Ganoderma lucidum crude polysaccharide powder, the crude polysaccharide purity is 52.6%.
Embodiment 2
[0030] Example 2: Concentration and separation of Ganoderma lucidum crude polysaccharide by ultrafiltration
[0031] ①Weigh 20 grams of Ganoderma lucidum fruiting body powder, add 560ML pure water and mix well, extract with an ultrasonic cell pulverizer (Zhejiang Ningbo Xinzhi Company; model: JY98-III ultrasonic cell pulverizer) at 680W for 17min, and remove the residue of Ganoderma lucidum by suction filtration;
[0032] ② The extract is subjected to 0.2 μm microfiltration to remove impurities such as organic colloids and mechanical particles.
[0033] ③ The permeate after microfiltration is subjected to ultrafiltration treatment, and the feed liquid is transported to the surface of the membrane (50kDa, with a membrane area of 0.1m2) by a peristaltic pump for phase tangential flow, the filtrate is collected, and the retained liquid is returned to the liquid storage tank. When the feed liquid is concentrated to about a quarter of its volume, the ultrafiltration process ends....
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Abstract
The invention discloses a micro-filtration and ultra-filtration combined efficient green purification technology for ganoderma lucidum polysaccharide. A ganoderma lucidum polysaccharide solution extracted by ultrasonic is used as an experimental material, and the extract is micro-filtered with meshes of 0.2 micron to remove impurities such as organic colloid and mechanical particles. The micro-filtered permeate is ultra-filtered, the liquid is delivered to the surface of a membrane (50kDa and the area of the membrane is 0.1m<2>) by a peristaltic pump to flow tangentially, the filtrate is collected, and the intercepted liquid is refluxed to a liquid storage tank; when the liquid is concentrated to about one fourth of volume, the ultra-filtration process is finished; and (95 percent) ethanol in a volume which is 4 times that of the liquid is used for precipitation, freeze-drying and weighing are carried out. The experimental conditions comprise that the temperature is 35 DEG C; in the operating pressure, the inlet pressure is 0.82bar, and the outlet pressure is 0.68bar; and the trans-membrane pressure difference delta P is equal to (0.82+0.68) / 2 and equal to 0.75bar. The purity of the ultra-filtered ganoderma lucidum polysaccharide reaches 61.32 percent, and concentration and purification processes of the ganoderma lucidum polysaccharide are greatly simplified. The obtained ganoderma lucidum polysaccharide can be widely applied to a base material for medicaments and health-care food.
Description
1. Technical field [0001] The invention relates to a method for concentrating and separating ganoderma polysaccharides. The obtained ganoderma polysaccharides have a purity greater than 60%, and the products can be widely used in the fields of health care products, functional foods, biomedicine and the like. 2. Background technology [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. So far, we have isolated more than 150 compounds from Ganoderma lucidum, which can be divided into polysaccharides, triterpenes, nucleosides, furans, alkaloids, amino acids, proteins, sterols, inorganic ions, organic germanium, etc. More than a dozen types of substances, polysaccharides have anti-tumor growth, reduce chemotherapy side effects, calm the nerves, improve immunity, strengthen the heart, lower blood lipids and blood pressure, p...
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