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High-density fermentation media for pediococcus pentosaceus and fermentation method

A technology of Pediococcus pentosaceus and high-density fermentation, applied in the field of microorganisms, can solve the problems of high cost of MRS medium and low number of viable bacteria, and achieve the effects of prolonging time, promoting accumulation and reducing production cost.

Inactive Publication Date: 2014-08-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on Pediococcus pentosacea is mainly concentrated in the production application, such as animal feed, silage, food, etc., but there are very few studies on the fermentation process of Pediococcus pentosacea, and there is no literature report yet. The traditional MRS medium is used for fermentation, but the cost of the traditional MRS medium is high, and the number of viable bacteria obtained in the end is less, and the number of viable bacteria is generally 1 billion / mL

Method used

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Experimental program
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Effect test

Embodiment 1

[0045] 1. Preparation of Pediococcus pentosaceus high-density fermentation medium

[0046] (1) Prepare materials according to the following formula

[0047] Peptone 15g, yeast powder 8g, sodium citrate 3g, sodium acetate 2g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.4g, corn steep liquor (industrial grade corn steep liquor, purchased from Yichang Huacheng Biological Fermentation Material Co., Ltd., protein content ≥ 45%, acidity ≤ 14%, sulfite ≤ 0.3%, ash ≤ 10%) 20g mix, dissolve all the solids with distilled water and set the volume to 900mL, add 5~8mol / L sodium hydroxide and 5~8mol / L Adjust the pH to 6.2 with hydrochloric acid, and sterilize at 103.4kPa steam pressure and 115°C for 30min to obtain the culture medium;

[0048] (2) Weigh 10g of glucose, dissolve it in distilled water and make up to 100mL, and sterilize at 103.4kPa steam pressure and 110℃ for 30min to obtain glucose solution;

[0049] (3) Add the glucose solution in step (2) to the sterilized med...

Embodiment 2

[0060] 1. Preparation of Pediococcus pentosaceus high-density fermentation medium

[0061] (1) Prepare materials according to the following formula

[0062] Peptone 10g, yeast powder 5g, sodium citrate 1g, sodium acetate 1g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, corn steep liquor (industrial grade corn steep liquor, purchased from Yichang Huacheng Biological Fermentation Material Co., Ltd., protein content ≥ 45%, acidity ≤ 14%, sulfite ≤ 0.3%, ash ≤ 10%) 20g mix, dissolve all the solids with distilled water and set the volume to 900mL, add 5~8mol / L sodium hydroxide and 5~8mol / L Adjust the pH to 6.4 with hydrochloric acid, and sterilize at 118°C for 25 minutes under 103.4kPa steam pressure to obtain the culture medium;

[0063] (2) Weigh 25g of glucose, dissolve it in distilled water and dilute to 100mL, sterilize at 113°C for 25min under 103.4kPa steam pressure to obtain glucose solution;

[0064] (3) Add the glucose solution in step (2) to the steriliz...

Embodiment 3

[0075] 1. Preparation of Pediococcus pentosaceus high-density fermentation medium

[0076] (1) Prepare materials according to the following formula

[0077] Peptone 10g, yeast powder 10g, sodium citrate 3g, sodium acetate 1g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, corn steep liquor (industrial grade corn steep liquor, purchased from Yichang Huacheng Biological Fermentation Material Co., Ltd., protein content ≥ 45%, acidity ≤ 14%, sulfite ≤ 0.3%, ash ≤ 10%) 20g mix, dissolve all the solids with distilled water and set the volume to 900mL, add 5~8mol / L sodium hydroxide and 5~8mol / L Adjust the pH to 6.6 with hydrochloric acid, and sterilize at 103.4kPa steam pressure and 121°C for 20min to obtain the culture medium;

[0078] (2) Weigh 40g of glucose, dissolve it in distilled water and dilute to 100mL, sterilize at 115°C for 20min under 103.4kPa steam pressure to obtain glucose solution;

[0079] (3) Add the glucose solution in step (2) to the sterilized med...

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Abstract

The invention belongs to the technical field of microorganism, and particularly discloses a high-density fermentation media for pediococcus pentosaceus and a fermentation method, which are suitable for the high-density fermentation production of the pediococcus pentosaceus. Fermentation process comprises the steps of preparing seed liquor and culturing in a fermentation cylinder. The phase of the culturing in the fermentation cylinder comprises pH adjustment and glucose feeding fermentation in the process of fermentation. In the process of fermentation, the pH of fermentation liquor is adjusted within the range of 5.0-6.5 via injecting NaOH solution with a certain concentration, glucose is supplemented intermittently after the fermentation for 4-6 hours, the fermentation is finished after culturing for 20-25 hours, and at this time, the number of effectively living bacteria can reach 9 billion / ml. By adopting the high-density fermentation media for pediococcus pentosaceus and the fermentation method provided by the invention, the bacterium number of the pediococcus pentosaceus is greatly increased.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high-density fermentation medium and a fermentation method of Pediococcus pentosaceae, which are suitable for high-density fermentation production of Pediococcus pentosaceae. Background technique [0002] Pediococcus pentosaceus (P.p) is a common lactic acid bacterium with spherical cells, no spore formation, no movement, facultative anaerobic, fermenting glucose, not producing gas, fermenting arabinose, ribose, maltose, etc., contact Enzyme positive, belonging to Gram-positive bacteria (Ling Daiwen, 1999, Lactic acid bacteria classification identification and experimental methods. China Light Industry Press). [0003] Pediococcus pentosaceae is considered a probiotic in animals and can be used directly in animal feeding, animal feed and silage. The advantages of natural lactic acid bacteria feed additives used in straw silage: shorten the fermentation cycle, quickly re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 陈守文冀志霞石娜娜祁高富马昕
Owner HUAZHONG AGRI UNIV