Method for detoxifying and reproducing helianthus tuberosus quickly
A technology of Jerusalem artichoke and Jerusalem artichoke tubers, which is applied in the field of detoxification and rapid propagation of Jerusalem artichoke, can solve the problems of low seed setting rate and germination rate, consume large tubers, increase planting costs, etc., achieve short induction of adventitious buds, shorten growth time, and reduce The effect of planting costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Store the harvested Jerusalem artichoke tubers at 4°C for 2 months, cut off the bud points and place them in a dark environment at 25-28°C for sand culture. After the sprouts start to germinate, transfer to light culture. 7-10cm, cut off and remove the leaves, rinse with tap water for one hour, and then carry out disinfection treatment: soak in 70% vt alcohol for 30s, rinse with sterile water three times, soak in 4%wt sodium hypochlorite for 5-7min, soak in sterile water Rinse 5 times. Test-tube seedling culture: cut out the stem tip and the stem segment with axillary buds, and insert them into the bud induction medium. When the adventitious buds grow to 2-3cm, they can be cut and transferred to the rooting medium for rooting induction, and finally the obtained complete test tube After hardening, the seedlings are transplanted to the field for cultivation and management.
[0022] The above-mentioned bud induction medium is obtained by adding Jerusalem artichoke tubers ...
Embodiment 2
[0028] Cut off the adventitious buds induced on the bud induction medium in Example 1 when they grow to 5-6cm, remove the leaves, cut off the stem segment or shoot tip with axillary buds as explants, and then transfer to the bud induction medium to carry out Value-added culture, after 5-10 generations of continuous value-added, rooting, seedling hardening, and finally transplanting to the field, these seedlings are still strong and maintain good vitality, and their basic characteristics are still consistent with the original mother plant. The specific implementation effect is shown in Table 2.
[0029] Table 2 Comparison of growth parameters between virus-free seedlings and non-virus-free seedlings
[0030]
Embodiment 3
[0032] A method for detoxification and rapid propagation of Jerusalem artichoke. The axillary buds or stem tips of Jerusalem artichoke are used as explants. After disinfection, they are transferred to a bud induction medium for test-tube seedling cultivation. When the buds grow to 2-3cm, the buds are transferred to Rooting medium, when the root grows to 3-4cm, the complete test-tube plantlet is hardened and transplanted into the field. The bud induction medium is based on MS, containing 30g L -1 Sucrose, 7g·L -1 Agar, 67g·L -1 Jerusalem artichoke tubers, 0.1mg·L -1 NAA and 0.5mg·L -1 6-BA, pH 5.8. The rooting medium is based on 1 / 2MS, containing 30mg L -1 Sucrose, 7mg·L -1 Agar, also contains 0.1mg·L -1 NAA or IBA, pH 5.6-5.7. The culture conditions of test tube seedlings are: temperature 25-28 ℃, light 16h d -1 , light intensity 1500-1800Lux. When the test tube seedling grows to 2-3cm, it can be directly cut off and transferred to the rooting medium for rooting cult...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com