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Method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by ultrasonic wave

A technology of lignocellulose and hydrogen peroxide, applied in the field of biomass resource utilization, can solve the problems of difficult lignin reduction and insufficient attention to lignin recycling, so as to reduce hindrance, save energy and Investment cost, effect of increasing ethanol yield

Inactive Publication Date: 2012-12-26
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional single pretreatment method is difficult to reduce the lignin in the lignocellulosic raw material to a minimum, and the recycling of the removed lignin has not been given sufficient attention, and the widely used acid pretreatment will produce furfural, HMF, etc. fermentation inhibitors

Method used

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  • Method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by ultrasonic wave
  • Method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by ultrasonic wave

Examples

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Effect test

Embodiment 1

[0032] Weigh 10g of dried corn cobs through a 40-mesh sieve, add 100ml, 0.25mol / L NaOH solution and 3.3ml 30% (v / v) H 2 o 2 Place it in a beaker and perform ultrasonic action at the same time. The applied power of ultrasonic wave is 120W, the action temperature is 25°C, and the total ultrasonic time is 40min. After the reaction, the slurry was subjected to vacuum filtration to achieve solid-liquid separation, and the filter residue generated above was repeatedly washed with distilled water until the pH value was neutral, and dried at 105° C. to constant weight.

[0033] Carry out paradigm analysis method (Van Soest) to measure the corn cob that pretreatment obtains (main components such as figure 2 shown) and enzymolysis, wherein the enzymolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50°C, pH 4.8 citric acid-sodium citrate buffer solution, cellulase Produced by Trichoderma reesei at a loading of 20 FPU / g dry material. After the enzymatic hydr...

Embodiment 2

[0035] Weigh 10g of corncobs after passing through a 40 mesh sieve and drying, add 100ml, 0.25mol / L NaOH solution into the beaker, and carry out ultrasonic wave at the same time. The time is 40min, and 3.3ml of 30% (v / v) H 2 o 2 . After the reaction, the slurry was subjected to vacuum filtration to realize solid-liquid separation, and the filter residue generated above was repeatedly washed with distilled water until the pH value was neutral, and dried at 105° C. to constant weight.

[0036] Carry out paradigm analysis method (Van Soest) to measure the corn cob that pretreatment obtains (main components such as figure 2 shown) and enzymatic hydrolysis, wherein the enzymatic hydrolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50°C, pH 4.8 citric acid-sodium citrate buffer solution, cellulase Produced by Trichoderma reesei at a loading of 20 FPU / g dry material. After the enzymatic hydrolysis, the reaction slurry was taken out, inactivated in a b...

Embodiment 3

[0038] Weigh 10g of corn cobs after passing through a 40-mesh sieve and drying, add 100ml, 0.25mol / L NaOH solution into the beaker, and perform ultrasonic action at the same time. The time is 40min, and 3.3ml of 30% (v / v) H 2 o 2 . After the reaction, the slurry was subjected to vacuum filtration to realize solid-liquid separation, and the filter residue generated above was repeatedly washed with distilled water until the pH value was neutral, and dried at 105° C. to constant weight.

[0039] Carry out paradigm analysis method (Van Soest) to measure the corn cob that pretreatment obtains (main components such as figure 2 shown) and enzymatic hydrolysis, wherein the enzymatic hydrolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50°C, pH 4.8 citric acid-sodium citrate buffer solution, cellulase Produced by Trichoderma reesei at a loading of 20 FPU / g dry material. After the enzymatic hydrolysis, the reaction slurry was taken out, inactivated in a bo...

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Abstract

The invention relates to a method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by an ultrasonic wave, and the method comprises the steps that when the lignocellulose is subjected to alkali treatment, the enhancing effect of the ultrasonic wave is conducted; after a period of time, the hydrogen peroxide is added for strong oxidization; and after the pretreatment is finished, a raw material is subjected to solid-liquid separation, wherein after a solid component in the raw material is dried, cellulase enzymolysis and composition analysis are conducted, and a liquid component is used for recovering hemicellulose and lignin components. The method aims at enhancing saponification and the strong oxidization of the alkaline hydrogen peroxide on the lignin component by fierce cavitation of the ultrasonic wave, so that the content of the lignin hindering the enzymolysis of a lignocellulose raw material is reduced greatly, most cellulose and hemicellulose are degraded to be fermentable monosaccharide, and the raw material utilization ratio of the lignocellulose is increased to the greatest extent. In addition, the hemicellulose and the lignin in pretreatment liquid are recovered by an acidulation method, and the green process without wasting of resources is realized.

Description

technical field [0001] The invention belongs to the technical field of biomass resource utilization, and relates to ultrasonic-strengthened alkaline hydrogen peroxide pretreatment. Specifically, the cavitation of ultrasonic waves is used to strengthen the saponification and strong oxidation of lignin components by alkaline hydrogen peroxide. To greatly reduce the lignin content, shorten the action time, and improve the efficiency of enzymatic hydrolysis. Background technique [0002] The second-generation fuel ethanol using lignocellulose as raw material can alleviate the increasingly severe energy shortage and environmental pollution problems on the one hand, and on the other hand can reduce the waste of lignocellulose raw materials and realize resource utilization. However, how to improve the hydrolysis rate and utilization rate of lignocellulosic raw materials in the production process is still a bottleneck restricting the development of second-generation fuel ethanol tec...

Claims

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Application Information

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IPC IPC(8): C12P7/10C08H7/00
CPCY02E50/16Y02E50/10
Inventor 仲兆平严青张茜芸邓学群艾特玲
Owner SOUTHEAST UNIV
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