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Natamycin preparation and preparation method thereof

A natamycin preparation technology, applied in the field of natamycin preparations and its preparation, can solve the problems such as the production method and related reports of natamycin preparations for mildew prevention in feed that have not been seen, and achieve less investment in fixed assets , easy storage, and simple equipment

Inactive Publication Date: 2013-01-23
湖北绿天地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are a few patents that occasionally mention that natamycin can be used for anti-mold in feed, but because of cost problems, no production method and related reports have been seen for the production of low-cost special natamycin preparations for anti-mold in feed, nor have Someone discloses a low-cost natamycin pesticide preparation production method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: 20% natamycin powder

[0066] 1. Fermentation liquid: at 10M 3 In a stainless steel fermenter, inoculate Streptomyces gilvosporeus (Streptomyces gilvosporeus) HBJ591 strain, ferment and produce natamycin, and the volume of the tank is 7.2M 3 , the tank potency is 12600mg / L;

[0067] 2. Adjust the pH value of the fermentation liquid: first use a refrigerator to lower the temperature of the fermentation liquid to 10°C, and measure the pH value of the fermentation liquid to be 4.86, then add Ca(OH) with a concentration of 2% (W / V) 2 While stirring the aqueous solution, adjust the pH value of the fermentation stock solution to 6.0, and control the temperature of the material to 10°C after adding alkali;

[0068] 3. Plate and frame filter press: the material is put into the plate frame for plate and frame filter press, and the filter cake is blown with cold wind for 2 hours, the supernatant is removed, and the filter cake is collected;

[0069] 4. Flash dryi...

Embodiment 2

[0074] Embodiment 2: 20% natamycin dry suspension

[0075] 1. Fermentation stoste: according to Example 1, Streptomyces luteus fermented stoste was prepared, with a titer of 14400 mg / L;

[0076] 2. Adjust the pH value: add Ca(OH) with a concentration of 2% (W / V) in 3.2 tons of fermentation stock solution 2 While stirring the aqueous solution, adjust the pH value of the fermentation stock solution to 5.5, then add 3.2kg chitosan for flocculation, and at the same time lower the temperature of the feed solution to 10°C;

[0077] 3. Plate and frame filter press: the specific method is the same as step 3 in Example 1.

[0078] 4. Flash drying: the method is the same as step 4 of Example 1, the flash drying temperature is 170° C., and the moisture content of the material is dried to 9.2%;

[0079] 5. Primary pulverization: use a hammer mill to pulverize the material to 100 mesh at room temperature, and the content of natamycin in the obtained natamycin dry powder is 29.91%; get 160k...

Embodiment 3

[0085] Embodiment 3: 10% natamycin suspension

[0086] 1, fermentation stoste: according to the method for embodiment 1, with Streptomyces gilvosporeus (Streptomyces gilvosporeus) HBJ591 bacterial strain fermentation stoste, fermented liquid potency 13400mg / L;

[0087] 2. Adjust the pH value of the fermentation stock solution and two-stage centrifugation: first use a refrigerator to lower the temperature of the fermentation stock solution to 10°C, and add Ca(OH) with a concentration of 2% to 7000kg of the fermentation stock solution 2 While stirring the aqueous solution, adjust the pH value of the fermentation stock solution to 6.5, then add 7kg chitosan to flocculate, stir, and let it stand at room temperature for 1.5 hours, then collect the precipitate, and add the supernatant to a disc centrifuge with a separation factor greater than 10000g Center centrifugation to obtain bacterial slurry (heavy liquid); because the titer of light liquid after centrifugation with a disc cen...

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Abstract

The invention relates to a natamycin preparation and a preparation method thereof. The preparation is prepared by the following method: utilizing an extraction-free method to separate a natamycin fermentation raw solution; adding or not adding a pesticide auxiliary agent into a concentrated solution or a filter cake of the natamycin fermentation raw solution and mixing uniformly to obtain the natamycin preparation; or uniformly mixing the obtained concentrated solution or the filter cake with other active components by adding or not adding the pesticide auxiliary agent to prepare a mixed preparation. The invention provides an extraction-free process for directly drying, puffing and crushing mycelia of natamycin production strains to produce the natamycin preparation which is specially used for fodders from mildew and is suitable for being used as a pesticide in the field. The extraction-free natamycin preparation production process has a simple process and does not use an organic solvent, and the required devices are fewer, so that the cost is low, the yield is high and no waste dreg is generated; and meanwhile, a flashing point of a product is high and the natamycin preparation is easy to store and transport, is safe and environment-friendly, and is beneficial to an ecological environment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a natamycin preparation and a preparation method thereof. Background technique [0002] Natamycin (Natamycin), also known as streptomycin, pimaricin or pimaricin, is an important polyene macrolide antibiotic. The antibiotic is a strong antifungal agent, which can effectively inhibit the growth of yeast and other fungi. Some fungi can produce mycotoxins (Mycotoxins). Mycotoxins have carcinogenic, teratogenic, shock-induced, hemorrhagic Cause dermatitis and other effects. Therefore, the use of natamycin in food preservation can not only solve food spoilage, but also reduce the toxicity of mycotoxins to the human body. Compared with other antibacterial ingredients, natamycin has extremely low toxicity to mammalian cells and can be widely used Diseases caused by fungi. In addition, due to the low solubility of natamycin, it can be used to treat the surface of food to increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00A23K1/17A23K20/195
Inventor 林开春杨国军孙松柏骆雪梅童文华江艳
Owner 湖北绿天地生物科技有限公司
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