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Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation

A biological preparation and refining technology, applied in textiles and papermaking, biochemical fiber treatment, fiber treatment, etc., can solve the problems of harsh workshop environment, pollution, unstable effect, etc., achieve excellent fiber structure, easy control of conditions, and improve degreasing effect of effect

Active Publication Date: 2013-01-30
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of serious pollution, unstable effect and bad workshop environment in the existing silk spinning and scouring method, and provide a kind of high-efficiency degreasing, high degree of automation, strong controllability, no damage to silk fibers, and no harm to the environment. Contaminated biological degreasing formulations and methods for their preparation and use

Method used

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  • Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In the present embodiment, the preparation method of the biological agent for degreasing by silk spinning comprises the following steps:

[0029] 1. The strain was inoculated into a lysis broth liquid culture bottle, placed on a constant temperature shaker, and cultured at 37°C and 150 r / min for 12 hours to obtain a seed liquid.

[0030] 2. Inoculate the seed liquid in proportion to 6% (volume ratio) in 1.5% bean cake flour liquid medium, and cultivate it for 84 hours at 37°C. When the number of viable bacteria fermented by culture is greater than 1 billion / ml, the bacterial strain is obtained fermentation broth.

[0031] 3. Centrifuge the fermentation liquid of the strain at 5500r / min for 8 minutes, discard the supernatant, and collect the wet bacteria as the sludge; mix the sludge with the freeze-drying agent, and the composition and ratio of the freeze-drying agent is 10% trehalose , 8% sucrose, 1% L-cysteine, 1% sodium glutamate. After mixing evenly, freeze-dry us...

Embodiment 2

[0043] In the present embodiment, the preparation method of the biological agent for degreasing by silk spinning comprises the following steps:

[0044] 1. The strain was inoculated into a liquid culture bottle of lysis broth, cultured with shaking at 38°C for 16 hours, and the morphology and purity of the strain were checked. Inoculate 6% (volume ratio) of qualified seed liquid in a liquid medium containing 2% beef extract and 1% maltose, and ferment at 37°C for 96 hours to obtain strain fermentation liquid.

[0045] 3. Centrifuge the fermentation broth of the strain at 8000r / min for 5 minutes, discard the supernatant, and collect the wet bacteria as the sludge; mix the sludge with sterilized anhydrous calcium chloride at a ratio of 1:1 and stir evenly , adding 5% sucrose, drying to a water content of 7%, and grinding into dry powder to obtain strain powder. After mixing evenly, it is freeze-dried in a vacuum freeze dryer at -5°C under aseptic conditions until the water cont...

Embodiment 3

[0055] The preparation conditions and process of this embodiment are basically the same as those of Example 1. Wherein, in this embodiment, the rotation speed of the shaker culture is 220r / min.

[0056] The biological preparation for silk spinning scouring and degreasing prepared in this example contains components and mass ratios as follows:

[0057] Strain powder 10 parts

[0058] The powder ratio of three strains of Bacillus cereus, Actinomyces A2016 and Pseudomonas fluorescens is 3:1:1

[0059] 50 parts corn stalk powder

[0060] 25 parts sawdust

[0061] Pseudomonas cepacia lipase 5 parts

[0062] 10 parts of UN-503 alkaline lipase.

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Abstract

The invention discloses a biological preparation for spinning silk and refining and removing oil. The biological preparation comprises the following components in parts by weight: 5-15 parts of strain powder, 5-15 parts of lipase and 70-90 parts of filler, wherein a strain comprises bacillus cereus, actinomycete A2016 and fluorescent fluorescent; the lipase is candida tropicalis extracellular lipase, onion pseudomonas lipase and UN-503 alkaline lipase; and the filler is anyone or random combination of corn stalk powder, cotton seed hull powder, wooden meal and soybean cake powder. The invention further discloses a preparation method and application of the biological preparation for spinning silk and refining and removing oil. The biological preparation for spinning silk and refining and removing oil, which is disclosed by the invention, has the advantages of high oil removing rate of silk spinning raw materials and no damage to silk fibers; and the refined wastewater has no harmful substances and no pollution to the environment.

Description

technical field [0001] The invention relates to a biological agent for silk spinning scouring and degreasing and a preparation method thereof, belonging to the technical field of primary processing of textile raw materials. Background technique [0002] Silk spinning raw materials come from the leftovers of sericulture, seed production, silk reeling, and silk weaving industries, including cocoons, long spit, stagnant heads, etc., which contain a lot of glue, high oil content, waxy, inorganic substances, dirt and other impurities. The scouring of silk spinning raw materials is to remove part of the sericin, most of the grease and impurities, open the glue point of the fiber, and make the silk fiber soft, fluffy, smooth and elastic, which is convenient for carding and spinning. The scouring effect of silk spinning raw materials is directly related to the output, quality and cost of silk spinning, and affects the economic benefits of enterprises. [0003] At present, the scour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00
Inventor 王映雪高红亮张美玲殷诚聪陶冶严芳琴谢润琦
Owner EAST CHINA NORMAL UNIV
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