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Preparation method of high-purity lecithin

A lecithin and high-purity technology, which is applied in the field of separation and extraction of natural products, can solve the problem of toxic and harmful solvents with limited operating scale of equipment, product purity that cannot meet the requirements of pharmaceutical-grade lecithin, and unsuitable large-scale high-purity lecithin. Production process promotion and use, etc.

Inactive Publication Date: 2013-02-13
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above-mentioned disclosed extraction methods are not suitable as high-purity lecithin for injection as a pharmaceutical excipient because the product purity does not meet the requirements of pharmaceutical-grade lecithin, or is limited by the operating scale of the equipment or the use of toxic and harmful solvents. Promoting and using large-scale production processes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Add 46.5 g of fresh egg yolk liquid into 155 ml of absolute ethanol, stir while adding, then suction filter to obtain filtrate and filter cake. The filter cake was put into a Soxhlet extraction device and extracted with absolute ethanol at 80° C. for 12 hours. The extract was rotary evaporated to remove the solvent and then combined with the filtrate to obtain a mixture of lecithin-containing lipid and ethanol. The mixture was left to stand at 4°C overnight for stratification, and then centrifuged at 3000 rpm for 30 min at 4°C to collect the supernatant. The supernatant is separated in a simulated moving bed chromatographic separation system composed of silica gel as an adsorbent and anhydrous ethanol and 70% (v / v) ethanol aqueous solution as a gradient eluent. The elution gradient is anhydrous ethanol: 70% (v / v) ethanol=10:1~1:9, to obtain a fraction containing lecithin and a fraction containing other components such as cephalin. The components containing lecithin ar...

example 2

[0025] The fresh egg yolk liquid is spray-dried to obtain egg yolk powder. Put 30g of egg yolk powder into a Soxhlet extraction device and extract with 96% (v / v) ethanol at 80° C. for 12 hours to obtain a mixture of lecithin-containing lipid and ethanol. The mixture was separated at 0°C overnight, then filtered at 10°C, and the filtrate was collected. The filtrate is separated in a simulated moving bed chromatographic separation system composed of polyacrylamide as an adsorbent and 96% ethanol solvent as an eluent to obtain a component containing lecithin and a component containing other components such as cephalin. The components containing lecithin are recovered on a rotary evaporator to remove the eluent, and then freeze-dried to obtain high-purity lecithin.

[0026] The lecithin has a purity of 98% (spectrally pure) through determination of phosphorus content and nitrogen content and high performance liquid phase inspection. The filter cake is combined with the component...

example 3

[0028] Add 10 g of commercially available soybean lecithin into 5000 ml of ethanol with a concentration of 70% (v / v), stir while adding, and then suction filter to obtain a mixture of lecithin-containing lipid and ethanol. The mixture was left to stand at 0°C overnight for stratification, and then centrifuged at 8000 rpm for 10 min at 4°C to collect the supernatant. The supernatant is separated in a simulated moving bed chromatographic separation system composed of activated alumina as an adsorbent and 70% (v / v) ethanol as an eluent to obtain components containing lecithin and other components containing cephalin and the like. Components of ingredients. The components containing lecithin are recovered on a rotary evaporator to remove the eluent, and then freeze-dried to obtain high-purity lecithin.

[0029] The lecithin has a purity of 98% (spectrally pure) through determination of phosphorus content and nitrogen content and high performance liquid phase inspection. The cent...

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Abstract

The invention belongs to the technical field of separation and extraction of natural products, and particularly relates to a preparation method of high-purity lecithin. The method is applied to the preparation of pharmaceutical high-purity lecithin. The invention provides a large-scale preparation and production method of high-purity lecithin for pharmaceutic adjuvant injection, adopts a simulated moving bed continuous chromatographic separation technology to ensure that lecithin is effectively separated from other constituents in material lipid, so as to obtain high-purity lecithin products, can also realize the recovery of residual products and the recycle of solvent, avoids the unreasonable consumption and waste of raw material and auxiliary material, completely eradicates the producing of wastewater and waste residue, ensures that the product yield and the product purity are more than 98 percent, ensures that the quality of the lecithin products meets the technical requirements of pharmaceutic adjuvant, greatly reduces the production cost, realizes the cleanability and no pollution of the lecithin production, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of natural products, in particular to a preparation method of high-purity lecithin. Background technique [0002] Lecithin, also known as phosphatidylcholine, is one of the phosphorus-containing polar lipids. It was first discovered by the French Gobley from egg yolk and brain in 1846-1847, and was separated from other phospholipids by Levenzai in 1925. [0003] Studies have shown that lecithin has many physical and chemical functions such as emulsification, solubilization, wetting, anti-oxidation, foaming, crystallization control, binding to protein, and preventing starch aging. In addition, lecithin is also a component of biological membranes and the main source of neurotransmitter acetylcholine, which can repair mitochondria and participate in body metabolism. At the same time, lecithin can also promote the combination of cholesterol and protein molecules, reduce the infiltra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 汤鲁宏赵绘绘曹雅晴滕霏
Owner JIANGNAN UNIV
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