Method of extracting succinic acid from microorganism fermentation liquor

Abstract

The invention discloses a method of extracting succinic acid from microorganism fermentation liquor. The method includes the following steps: (1) sodium succinate fermentation liquor obtained through microorganism fermentation is clarified through an ultrafiltration membrane or micro-filtration membrane for removing thalli or other large-particle impurities; (2) the clarified fermentation liquor is acidized through concentrated hydrochloric acid to reach pH ranging from 1 to 3, and the pH is preferentially 2.0; (3) the acidized liquor is subjected to reduced pressure distillation concentration to be with succinic acid concentration at 300-400g / L, and sodium chloride precipitations are removed through solid-liquid separation; (4) the filter liquor obtained in the step (3) is subjected to cooling crystallization under stirring at temperature to be 5 DEG C to obtain succinic acid solids; (5) the filter liquor obtained in the step (4) continues to be concentrated to obtain second sodium chloride precipitations; (6) the residual mother liquor is subjected to cyclic operation according to the step (4) and the step (5) to obtain succinic acid solids and sodium chloride solids; and (7) succinic acid solids and sodium chloride solids are dissolved, discolored and recrystallized to obtain high-purity succinic acid crystals and sodium chloride crystals.

Description

technical field [0001] The invention relates to a method for extracting succinic acid from microbial fermentation broth. Background technique [0002] Succinic acid, also known as succinic acid, is an important C4 platform compound, one of the 12 most important platform compounds announced by the U.S. Department of Energy. At present, there are four main markets for succinic acid: first, as surface active agent, cleaning agent, additive and foaming agent; second, as an ion chelating agent, used to prevent metal corrosion and pitting in the electroplating industry; third, in the food industry as an acidifier, pH modifier, flavoring substance and Antibacterial agents; the fourth is health-related products, including medicines, antibiotics, amino acids and vitamins. These four market shares of succinic acid total more than $400 million / year. [0003] Succinic acid is also an important chemical synthesis raw material that can replace many commodities based on benzene and petro...

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Problems solved by technology

[0005] But above-mentioned separation and extraction method all has various shortcomings: 1) the advantage of calcium salt method is that equipment investment is little, operating cost is lower, and defect is that extraction steps are loaded down with trivial details, and labor intensity is big, and yield is low, produces a large amount of 2) the extraction yield of the ammonium salt method is higher, but the operation steps are loaded down with trivial details, difficulty is big, the operating cost is high, and the cost of aftertreatment is high; 3) the extraction yield and the product purity of the electrodialysis method are very high High, the defect is high energy consumption, and the treatment of impurity ions has great limitations, such as not being able to deal with divalent ions, the equipment investment is large, and the operating cost is high; 4) The advantage of the solvent extraction method is that the early equipment investment is small and the product purity is high However, a large amount of expensive organic solvents need to be consumed during the extraction process, resulting in higher purification costs. At the same time, the toxicity of residual solvents has a negative impact on the quality of food-grade and pharmaceutical-grade products. This method cannot completely remove impurities; 5) Membrane The heteroacids in the separation method cannot be removed at all, the yield is low, and the investment cost is relatively high; 6) the yield of the ion exchange method is very high, but because the concentration of succinic acid is low, the evaporation cost is high, and the life of the ion exchange resin is short. Push up operating costs, in addition, a large amount of waste water is generated during the resin regeneration process

[0006] In the above extraction methods, there may be large investment in equipment, high operating costs, or low product yield and purity, or cumbersome steps, high labor costs, or a large amount of waste and waste water are generated, and environmental protection pressure is high, which is not conducive to the production of succinic acid by fermentation process of industrialization

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