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Scenedesmus sp. and use thereof

A technology of Scenedesmus, dry weight, applied in the field of microorganisms, can solve the problems of low processing capacity and performance, and achieve the effects of fast oil accumulation, high oil content and high utilization rate

Active Publication Date: 2013-02-27
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although microalgae have very good development prospects in energy, environmental protection, and even medicine, and related research work has been gradually carried out, the currently known microalgae, especially Scenedesmus, have a great influence on oil content, CO 2 The performance in terms of processing capacity and other aspects is generally low, so there is a need for Scenedesmus with better performance and more suitable for industrial applications.

Method used

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  • Scenedesmus sp. and use thereof
  • Scenedesmus sp. and use thereof
  • Scenedesmus sp. and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take a water sample from Dianchi Lake in Yunnan, add 1 / 3 of the volume of BG11 medium, and culture it for about 5 days at 25°C and a light intensity of 40μmol / m2.s. Take the water sample and observe it under a microscope to judge the water sample. main species present in . Adjust the cell concentration to about 1000 cells / ml, take 100 μl of water sample and spread it on the solid agar plate of BG11 medium, at 25°C, the light intensity is 40 μmol / m 2 After culturing for about 10 days under the condition of about .s, a single algae colony can be seen to grow. Use an inoculation loop or a sterile toothpick to pick a single algae colony and inoculate it in BG11 medium for culture. This process is repeated until a purely cultured algal strain is obtained, which is numbered "ENN34" in the present invention.

Embodiment 2

[0038] Example 2 Identification of algae species and their classification on the phylogenetic tree.

[0039] Identification of algae strains takes a combination of morphological identification and molecular identification.

[0040] 1. Morphological identification (see figure 1 ): The algae strain ENN34 isolated above was observed under a 1000-fold microscope. The cells were spherical, 6-10um in size, with perichrome, a pyrenoid, and asexual reproduction to produce spore-like spores. Initially identified as Scenedesmus.

[0041] 2. Molecular identification:

[0042] A, Amplification of 18S nucleotide sequences from isolated algae

[0043] Genomic DNA of ENN 34 algae cultured for 4 days was extracted by CTAB method. Take a certain amount of cells, add an appropriate amount of CTAB buffer after grinding, homogenate, add an equal volume of phenol:chloroform for extraction, an equal volume of isopropanol for precipitation, wash with 75% ethanol and dissolve in a certain volume ...

example 3

[0055] Indoor culture, oil accumulation and pigment analysis of example 3 ENN34 algae:

[0056]The green cells of Scenedesmus strain ENN 34 in the logarithmic growth phase were inoculated in the prepared BG11 medium (see Table 1 for the formula), so that the cell density reached an OD750 of 0.2-0.8. During the cultivation process, the light intensity is controlled at 50-500umol / m2.s. During the cultivation period, the pH value of the medium is adjusted between 7-9 by introducing a mixture of 1.5-2% carbon dioxide and air into the culture medium. During the period, the temperature is controlled within the range of 15-35°C. The reactor used for the cultivation was a column reactor with an inner diameter of 40 mm and a length of 600 mm. During the cultivation process, samples were taken regularly to determine the dry weight, and the results were shown in Figure 4 . On the 16th day of cultivation, when the color of the algae liquid turns red, the algae liquid is collected, and...

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Abstract

The invention discloses scenedesmus sp. and a use thereof. The scenedesmus sp. has a fast growth speed, a fast grease accumulation speed and high content, can effectively remove nitrogen-containing compounds in water, contains an appropriate amount of beneficial pigments, and can be used for production of biodiesel, foods, health-care products, feed, aquatic product baits, and chemical products such as cosmetics and pigment products.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Scenedesmus ENN34 (Scenedesmus sp.) and its application. Background technique [0002] With the gradual depletion of the world's fossil fuel reserves, the continuous increase of carbon dioxide emissions and the increasing warming of the global climate, human survival and development have faced unprecedented challenges. Therefore, countries all over the world are actively looking for renewable energy sources that can replace petroleum products. Among them, biodiesel is an important bioenergy source. If there are too many, the problem of "competing with people for food and land with food" will arise, which will lead to the embarrassing result of "the energy crisis has been solved, but the food crisis has emerged". [0003] Microalgae is a kind of low-level plants that are widely distributed on land and in the ocean, rich in nutrients, short in growth cycle, and high in photosynthetic...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12P7/06C02F3/32B01D53/84B01D53/62C12P23/00A23L1/30A23K1/16A01K97/04A61K36/05A61K8/97C12R1/89
CPCY02E50/17Y02E50/13Y02A50/20Y02E50/10Y02P20/59
Inventor 罗少敬黄龙耀马建源吴洪邓平
Owner ENN SCI & TECH DEV
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