Scenedesmus sp. and use thereof

A technology of Scenedesmus, dry weight, applied in the field of microorganisms, can solve the problems of low processing capacity and performance, and achieve the effects of fast oil accumulation, high oil content and high utilization rate

Active Publication Date: 2013-02-27
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although microalgae have very good development prospects in energy, environmental protection, and even medicine, and related research work has been gradually carried out, the currently known microalgae, especially Scenedesmus,

Method used

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  • Scenedesmus sp. and use thereof
  • Scenedesmus sp. and use thereof
  • Scenedesmus sp. and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0063] After the drying is completed, determine the content of its oil components as shown in Table 2, and the analysis method is as follows:

[0037] Take a water sample from Dianchi Lake in Yunnan, add 1 / 3 volume of BG11 medium, culture for about 5 days at 25°C and light intensity of 40μmol / m2.s, take the water sample and observe under a microscope to judge the water sample The main species present in. Adjust the cell concentration to about 1000 cells / ml, take 100μl water sample and spread it on the solid agar plate of BG11 medium, at 25℃, the light intensity is 40μmol / m 2 After culturing for about 10 days under the condition of about .s, you can see the colonies of single algae grow. Use an inoculating loop or a sterile toothpick to pick the colonies of single algae and inoculate them in BG11 medium. Repeat this process until a purely cultured algae strain is obtained, which is numbered "ENN34" in the present invention.

Example Embodiment

[0038] Example 2 Identification of algae species and their classification on the evolutionary tree.

[0039] The identification of algae strains adopts a combination of morphological identification and molecular identification.

[0040] 1. Morphological identification (see figure 1 ): Observe the isolated algae strain ENN34 under 1000 magnification. The cells are spherical, 6-10um in size, perichromosomal, a protein nucleus, and asexual reproduction produces parent-like spores. It was initially identified as Scenedesmus.

[0041] 2. Molecular identification:

[0042] A, Amplify its 18S nucleotide sequence from the isolated algae

[0043] The genomic DNA of ENN 34 algae cultured for 4 days was extracted by CTAB method. Take a certain amount of cells, add an appropriate amount of CTAB buffer after grinding treatment, homogenize, add an equal volume of phenol: chloroform extraction, equal volume of isopropanol precipitation, wash with 75% ethanol and dissolve in a certain volume of steri...

Example Embodiment

[0055] Example 3 Indoor cultivation, oil accumulation and pigment analysis of ENN34 algae:

[0056] The green cells of Scenedesmus ENN 34 in the logarithmic growth phase were inoculated in the prepared BG11 medium (see Table 1 for the formula), so that the cell density reached an OD750 of 0.2-0.8. During the cultivation process, the light intensity is controlled at 50-500umol / m2.s. During the cultivation period, the pH value of the medium is adjusted to 7-9 by passing 1.5-2% carbon dioxide and air mixed gas into the culture solution. In between, the temperature is regulated within the range of 15-35°C. The reactor used for the culture is a column reactor with an inner diameter of 40 mm and a length of 600 mm. During the cultivation process, samples are taken regularly to determine the dry weight, and the results are shown in Figure 4 . When the culture progresses to the 16th day, when the color of the algae liquid turns red, the algae liquid is collected, and the algae mud is ...

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Abstract

The invention discloses scenedesmus sp. and a use thereof. The scenedesmus sp. has a fast growth speed, a fast grease accumulation speed and high content, can effectively remove nitrogen-containing compounds in water, contains an appropriate amount of beneficial pigments, and can be used for production of biodiesel, foods, health-care products, feed, aquatic product baits, and chemical products such as cosmetics and pigment products.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Scenedesmus ENN34 (Scenedesmus sp.) and its application. Background technique [0002] With the gradual depletion of the world's fossil fuel reserves, the continuous increase of carbon dioxide emissions and the increasing warming of the global climate, human survival and development have faced unprecedented challenges. Therefore, countries all over the world are actively looking for renewable energy sources that can replace petroleum products. Among them, biodiesel is an important bioenergy source. If there are too many, the problem of "competing with people for food and land with food" will arise, which will lead to the embarrassing result of "the energy crisis has been solved, but the food crisis has emerged". [0003] Microalgae is a kind of low-level plants that are widely distributed on land and in the ocean, rich in nutrients, short in growth cycle, and high in photosynthetic...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12P7/06C02F3/32B01D53/84B01D53/62C12P23/00A23L1/30A23K1/16A01K97/04A61K36/05A61K8/97C12R1/89
CPCY02E50/17Y02E50/13Y02A50/20Y02E50/10Y02P20/59
Inventor 罗少敬黄龙耀马建源吴洪邓平
Owner ENN SCI & TECH DEV
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