Biological preparation method for natural safe starch nano particles
A starch nano-biological preparation technology, applied in the field of nano-structured materials, can solve the problems of high toxicity, unfavorable large-scale industrial production, and increased cost of starch nano-particle preparation, and achieve the goal of shortening the production cycle, reducing requirements, and reducing the amount of use Effect
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[0021] Example 1
[0022] Weigh 10g of waxy corn starch and add it to 200mL of a pH 4.6 disodium hydrogen phosphate-citric acid buffer solution, heat to completely gelatinize the starch milk, and cool to 60°C, which is the optimum temperature for the enzyme reaction. Add Pullulanase, enzymatically hydrolyze for 4 hours, increase the temperature to inactivate the enzyme, centrifuge at low speed for 10 minutes while it is hot, remove the long-chain starch, and then slowly cool down, at this time the short-chain amylose will aggregate each other to form crystals, and aggregate at room temperature After 15 hours, it was centrifuged at 2000r / min for 20 minutes, the supernatant was centrifuged at 5000r / min for 20 minutes, and the obtained precipitate was vacuum freeze-dried to obtain starch nanoparticles of 20-500nm.
Example Embodiment
[0023] Example 2
[0024] Weigh 20g of sweet potato starch, place it in 400mL of citric acid-sodium citrate buffer solution with pH 4.6, heat it to gelatinize completely, then reduce the temperature of the water bath to 60℃, add isoamylase, and react for 4 hours, then Add amylase to react for 2 hours, raise the temperature to 90°C to make the enzyme activity completely disappear, centrifuge at low speed for 15 minutes while it is hot, remove the long chain starch that has not reacted completely, then slowly lower the temperature to 5°C, and let stand at this temperature After 10 hours, the short amylose was fully crystallized, and then centrifuged at 3000r / min for 20 minutes to obtain a precipitate. The precipitate was dissolved, and the solution was subjected to ultrasonic treatment. Finally, the solution after the treatment was vacuum freeze-dried. After 15 hours, 100 ~800nm starch nanoparticles.
Example Embodiment
[0025] Example 3
[0026] Weigh 10g of potato starch and disperse it in 200mL of a pH 4.6 disodium hydrogen phosphate-citric acid buffer solution. Heat the starch milk to 100°C. After the starch is completely gelatinized, cool to 60°C, and add pullulan enzyme and starch Enzyme, reacted for 5 hours, amylase can cut long chain starch, pullulanase removes the branched chain of amylopectin, then raise the temperature to 85℃, high temperature inactivate the enzyme, after 20 minutes, at a rate of 2℃ per minute Slowly lower the temperature and let it stand for 12 hours when the temperature reaches 5°C. This process is the stage of short starch chain agglutination, and then centrifuge at 3000r / min for 20 minutes to obtain a precipitate. The precipitate is dispersed in acetone and the dispersion is placed Drying in a blast drying oven for 4 hours, 20-600nm starch nanoparticles can be obtained.
[0027] The use of biological enzymes to prepare starch nanoparticles greatly shortens the produ...
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