Rapid paraffin sectioning method for plant tissue

Abstract

The invention discloses a rapid paraffin sectioning method for plant tissue. The method comprises the following steps: sampling, fixation, dehydration and vitrification, paraffin embedding and sectioning, gluing tablets and spreading tablets, and dyeing and cementing. The invention greatly improves fixation efficiency of paraffin sectioning for plant tissue by 10-20 times, compared with a fixation time of one day in traditional paraffin sectioning; tert-butyl alcohol or n-butanol is used as a dehydrating agent and a transparent agent to simplify the dehydration and vitrification step, and the dehydration and vitrification only consumes 6-7h, which has efficiency improved by more than 3 times compared with a traditional dehydration and vitrification; and infiltration efficiency of the plant tissue in paraffin and embedding quality are improved, and the penetration consumes 4-7h, which has efficiency improved by more than one time compared with the traditional paraffin infiltration method, so as to effectively solve problems of tissue hardening and brittleness caused by high temperature during the paraffin infiltration. The invention has the characteristics of simplified experimental procedures, low equipment price, short production cycle, high sectioning quality, safety and environment-friendliness.

Description

technical field [0001] The invention relates to the technical field of paraffin-wax preparation of biological tissues, in particular to a method for fast paraffin-wax preparation of plant tissues. Background technique [0002] The application and development of paraffin wafer technology has a history of 150 years. It is widely used in human, animal, plant, insect, microorganism and other research fields, because biological tissue materials can be made into very thin and continuous slices, and the slide material can be preserved for decades. Therefore, it has become the most perfect film-making method in biological research. Traditional paraffin technology is widely used in botany, plant anatomy, plant pathology and other disciplines. The process includes: fixation, dehydration, transparency, paraffin penetration, embedding, slicing, sticking, spreading, baking, melting wax, staining and sealing. However, the traditional paraffin slice technology also has disadvantages: th...

AI Technical Summary

Problems solved by technology

However, the traditional paraffin wax sectioning technology also has disadvantages: the sectioning steps are cumbersome and lengthy, and the required sectioning equipment is relatively expensive. Usually, biological tissue sections need to be equipped with biological tissue dehydrators, embedding machines, baking machines, and wax melting boxes. equipment; the production process takes a long time, generally takes 3 to 5 days or even longer, and the operation process is complicated, especially the steps of fixation, dehydration, transparency, wax penetration, etc., which consume the most time in the entire production process. Each step needs to be within the specified time, and the producer must wait for the various reagents to be replaced slowly in the tissue material, which is easy to make the tissue shrink, harden and become brittle, and it is difficult to meet hard or brittle materials. Cut out ideal slices

At the same time, in the process of film production, a variety of toxic organic chemical reagents, such as xylene, glacial acetic acid, n-butanol and paraffin, have been used in large quantities for a long time, which not only causes laboratory air pollution but also seriously threatens the health of film producers