Method for producing heme iron by using duck blood
A technology of heme iron and duck blood is applied in the field of heme iron production, which can solve the problems such as no public report of heme iron, and achieve the effect of increasing added value.
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[0017] (1) Anticoagulation and antioxidation treatment of duck blood: 2 g / L ascorbic acid was used as an antioxidant, and the addition amount was 0.05%-0.1% of the blood volume; and 4 g / L trisodium citrate was used as an anticoagulant, The added amount is 16.7% of the blood volume;
[0018] (2) Centrifugation: centrifuge at 4000r / min for 30min, the supernatant is plasma, and the centrifuged sediment is blood cells;
[0019] (3) Hemolysis and cleavage of blood cells: Part of the blood cells are hemolyzed by heating and cooling the precipitated blood cells obtained by centrifugation at -20°C and 90°C, and then homogenized at 120MPa dynamic ultra-high pressure micro jet Under-machine treatment once for cell destruction to obtain hemoglobin;
[0020] (4) Stage enzymatic hydrolysis control: divided into two stages, the first stage is at pH 8.5, temperature 55°C, using 0.5% alkaline protease for 2.5h; the second stage is at pH 7.0, reaction temperature 50°C , use 0.3% neutral prot...
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