Green synthesis method of germanium quantum dot in solution, germanium quantum dot and application thereof in biological imaging

Abstract

The invention provides a green synthesis method of a germanium quantum dot in a solution, a germanium quantum dot and application thereof in biological imaging. The green synthesis method comprises the following step: by taking germanium dioxide as a germanium source, polyvinylpyrrolidone as a protective agent and sodium borohydride as a reducing agent, preparing to obtain the germanium quantum dot. According to the invention, the used raw materials are simple, accessible and stable in property, and basically has no biological toxicity; high temperature and protection of inert gas are not required in the reaction process, thereby ensuring that the green synthesis method is simple and easy to operate; the prepared germanium quantum dot having blue fluorescence has a quantum yield of 26% or above, can be easily separated from the system, has no cytotoxicity and can be well used in biological cell imaging.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and relates to a synthesis method of germanium quantum dots, in particular to a synthesis method of germanium quantum dots in a solution, especially a green synthesis method, germanium quantum dots and their application in biological imaging. Background technique [0002] Silicon and germanium of the fourth main group, as indirect bandgap semiconductor materials, have been widely used in the field of microelectronics in the past few decades, but due to the extremely low fluorescence quantum yield at room temperature, their applications in the field of optical and optoelectronic devices has always been limited. In recent years, with the rapid development of nanotechnology, new breakthroughs have been made in the research of semiconductor materials silicon and germanium. When they are at the nanometer scale, due to the quantum confinement effect, they will transform into nanomaterials with di...

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Problems solved by technology

[0002] Silicon and germanium of the fourth main group, as indirect bandgap semiconductor materials, have been widely used in the field of microelectronics in the past few decades, but due to the extremely low fluorescence quantum yield at room temperature, their applications in the field of optical and optoelectronic devices has been limited

However, there are many problems in the existing germanium preparation methods. For example, the germanium sources used in some preparation methods (such as germanium tetrachloride, germanium diiodide, etc.) are highly toxic and difficult to preserve, and the preparation conditions of some preparation methods are very harsh ( Such as requiring inert gas protection, high reaction temperature, high boiling point solvent, etc.), and most of the preparation reactions are carried out in the organic phase, which brings great limitations to the separation and application of quantum dots, especially in cells Imaging field

There are also working groups that modify the surface of germanium quantum dots prepared in the organic phase into amino groups to make them soluble in water. However, due to the presence of a large number of amino groups on the surface, it has great cytotoxicity, which also limits its biological application. Applications in Cell Imaging

At the same time, the germanium quantum dots prepared by many methods cannot exist stably in the atmosphere, and will be oxidized in the air, the fluorescence will be gradually quenched, and the fluorescence quantum yield is low, which also limits the application of germanium quantum dots

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