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Application of bacteroides fragilis in preparing composition for preventing and treating acute radiation enteritis

A technology of Bacteroides fragilis and radiation enteritis, applied in application, food preparation, drug combination, etc., to achieve good dietary and medicinal prospects, good therapeutic effect, and strong pharmacological effect

Inactive Publication Date: 2013-06-12
广东知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bacteroides fragilis is used as a probiotic, and its application in the preparation of compositions for treating acute radiation enteritis has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Bacteroides fragilis bacterial liquid

[0018] (1) Isolation of Bacteroides fragilis from infant feces

[0019] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. A single colony was selected for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0020] (2) Enrichment culture of Bacteroides fragilis and preparation of bacterial solution

[0021] Select the purified single colony and inoculate it in the improved tryptone broth for enrichment culture. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for 15 minutes, and the supernatant is...

Embodiment 2

[0022] Example 2 Toxicity test of Bacteroides fragilis

[0023] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0024] (1) Acute toxicity test in mice

[0025] Sixty C57BR / C mice weighing 22±2 g (half male and half male) were divided into high-dose group, low-dose group and control group, 20 in each group. Each mouse in the high-dose group was intragastrically administered 10 12 cfu / ml bacterial solution 0.5ml, the low dose group was administered orally 10 times a day 9 cfu / ml bacterial solution 0.5ml, and the control group was fed with 0.5ml normal saline every day. Continuous observation for 14 days.

[0026] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0027] (2) Rat toxicity test

[0028] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. Each rat in the high-do...

Embodiment 3

[0033] Example 3 The therapeutic effect of Bacteroides fragilis on acute radiation enteritis

[0034] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0035] Model establishment and grouping: 150 SD rats were randomly divided into 3 groups: group A was the normal control group (30 rats), group B was the normal dose irradiation group (60 rats), and group C was the high dose irradiation group (60 rats). Rats were fasted for 12-14 hours, weighed, anesthetized with chloral hydrate (0.3ml / 100g) intraperitoneally, fixed on a plexiglass plate in a supine position, and irradiated the whole abdomen with a high-energy X-ray linear accelerator, from the xiphoid process to the Pubic symphysis, irradiation area 7*5cm 2 , the remaining parts are shielded with 5cm thick lead plates, the source-to-skin distance is 98cm, and the dose rate is 500cGy / min. The irradiation dose of group B was 10Gy, the irradiation dose of group...

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PUM

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Abstract

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparing a composition for preventing and treating acute radiation enteritis. Experiments show that bacteroides fragilis is safe, nontoxic and strong in pharmacologic action and has a good preventing and treating effect to acute radiation enteritis, thereby indicating that the bacteroides fragilis has good edible and officinal prospects. The invention explores novel use of bacteroides fragilis and develops a novel application field. Bacteroides fragilis serving as probiotic can be used for preparing foods or medical compositions for preventing and treating acute radiation enteritis.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing a composition for treating acute radiation enteritis. Background technique [0002] Radiation therapy is one of the important treatment methods for tumors. About 60%-70% of cancer patients need radiation therapy at different stages of the disease course, and radiation enteritis is a common complication after radiation therapy for pelvic, abdominal or retroperitoneal malignant tumors. The small intestine, colon, and rectum can be involved respectively, so it is also called radiation rectum, colon, and enteritis. Gastrointestinal symptoms can appear early on due to the nervous system's response to radiation. It usually occurs within 1 to 2 weeks after the start of radiotherapy. Nausea, vomiting, diarrhea, passing mucus or bloody stools. Those who involve the rectum are accompanied by tenesmus. Per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P1/00A23L1/30A23C9/152A23C9/13A23C19/00A23G9/36
Inventor 智发朝白杨刘洋洋
Owner 广东知光生物科技有限公司
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