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Preparation of composite hydrogel tissue engineer cartilage repairing scaffold with biological activity

A composite hydrogel and cartilage repair technology, applied in medical science, prosthesis and other directions, can solve the problems of no biological activity, weak mechanical strength of PVA hydrogel, affecting cartilage fixation and repair function, etc., and achieves a simple and good process. Bioactive, low-cost effects

Active Publication Date: 2013-06-12
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with human articular cartilage, PVA hydrogel has no biological activity, which affects the fixation and repair function of cartilage, and the mechanical strength of PVA hydrogel is weak. It is difficult to control its appearance and microstructure when used as a tissue engineering scaffold.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The cartilage tissue to be repaired is scanned by computerized tomography technology, and a digital model of the three-dimensional structure of the cartilage tissue is obtained. The three-dimensional porous structure inside the scaffold material is designed by using the digital model. There are 9×9 arrays of micropores on every 5mm×5mm processing plane, the diameter of the micropores is 500 μm, and the spacing between the micropores is not less than 200 μm. The negative shape of the designed cartilage repair bracket was obtained through Boolean operations, and the paraffin negative shape of the cartilage repair bracket was made by using 3D rapid prototyping technology and a 3D rapid printer with paraffin powder as the molding material.

[0025] Acetobacter xylinum that can secrete bacterial cellulose was selected, and the strain was activated to prepare seed mash. The seed mash with a strain concentration of 50% was evenly added dropwise on the paraffin negative type in ...

Embodiment 2

[0028] The cartilage tissue to be repaired is scanned by computerized tomography technology, and a digital model of the three-dimensional structure of the cartilage tissue is obtained. Use the digital model to design the three-dimensional porous structure inside the scaffold material. There are 6×6 array micropores on every 5mm×5mm processing plane, the diameter of the micropores is 200 μm, and the distance between the micropores is not less than 200 μm. The negative shape of the designed cartilage repair bracket was obtained through Boolean operations, and the paraffin negative shape of the cartilage repair bracket was made by using 3D rapid prototyping technology and a 3D rapid printer with paraffin powder as the molding material.

[0029] Select the Rhizobium genus that can secrete bacterial cellulose, activate the strain to prepare seed mash, and evenly drop the seed mash with a strain concentration of 95% on the paraffin negative type in a lattice manner, and add the ferme...

Embodiment 3

[0032] The cartilage tissue to be repaired is scanned by computerized tomography technology, and a digital model of the three-dimensional structure of the cartilage tissue is obtained. The three-dimensional porous structure inside the scaffold material is designed by using the digital model. There are 7×7 arrays of micropores on every 5mm×5mm processing plane, the diameter of the micropores is 250 μm, and the distance between the micropores is not less than 200 μm. The negative shape of the designed cartilage repair bracket was obtained through Boolean operations, and the paraffin negative shape of the cartilage repair bracket was made by using 3D rapid prototyping technology and a 3D rapid printer with paraffin powder as the molding material.

[0033] Select the Pseudomonas genus that can secrete bacterial cellulose, activate the strain to prepare seed mash, and evenly drop the seed mash with a strain concentration of 65% on the paraffin negative type in a dot matrix manner, a...

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Abstract

The invention discloses a preparation method of a composite hydrogel tissue engineer cartilage repairing scaffold with biological activity. The preparation method comprises the steps of: preparing a paraffin model of the cartilage repairing scaffold; by taking the paraffin model as a template, selecting and fermenting-cultivating a bacterial strain capable of secreting bacterial cellulose; removing paraffin from a fermentation product, and purifying, dewatering and immersing the fermentation product, thus obtaining a bacterial cellulose hydrogel cartilage repairing scaffold containing 30-50% of CaC12 aqueous solution by weight; dissolving polyvinyl alcohol in an Na2HPO4 aqueous solution, thus obtaining a mixed solution A; enabling the mixed solution A to enter the bacterial cellulose hydrogel cartilage repairing scaffold by virtue of an immersion method to obtain a product; freezing-unfreezing the obtained product for multiple times, and irradiating the product by gamma rays, thus obtaining the composite hydrogel tissue engineer cartilage repairing scaffold with biological activity. Composite hydrogel prepared by the method has an appropriate appearance, a porous structure, an excellent mechanical property and excellent biological activity and can be used as a tissue engineer scaffold material for repairing cartilage tissue.

Description

technical field [0001] The invention relates to the field of composite preparation of biomaterials, in particular to a method for preparing a bioactive composite hydrogel tissue engineering cartilage repair scaffold. Background technique [0002] Cartilage defects are difficult to self-repair, causing great pain to patients. The repair of cartilage defects is a thorny problem that plagues clinical treatment. There are a large number of such patients worldwide, and with the aging of society, the incidence of cartilage defects is showing an increasing trend. Clinically, cartilage defect repair mainly adopts autologous, allogeneic cartilage transplantation and joint prosthesis replacement. The clinical application of autologous cartilage transplantation is limited due to the limited source of cartilage tissue. Although the source of cartilage tissue is sufficient for allogeneic cartilage transplantation, there is a certain problem of immune rejection in this method; in additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/16A61L27/12A61L27/56
Inventor 钟春燕其他发明人请求不公开姓名
Owner 钟春燕
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