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Preparation method and application of microbial manganese-carbon composite material

A composite material and microorganism technology, which is applied to the preparation of microbial-based manganese-carbon composite materials, and the application field of microbial-based manganese-carbon composite materials, can solve problems such as the preparation of environmental materials by manganese-oxidizing bacteria and their metabolites that have not yet been found, and achieve good practicality. Value and application prospects, fast response, no secondary pollution effect

Inactive Publication Date: 2013-06-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research has been found on the preparation of environmental materials from manganese oxidizing bacteria and their metabolites

Method used

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  • Preparation method and application of microbial manganese-carbon composite material
  • Preparation method and application of microbial manganese-carbon composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing a microbial-based manganese-carbon composite material, the preparation steps of which are:

[0021] (1) Add a certain amount of filter-sterilized HEPES buffer (pH 6-7) and 1ml1mol / lMnCl under sterile conditions 2 In the sterilized culture medium, the final concentration was 10mmol / L, and at the same time, 1% of the inoculum was inserted into the bacterial solution of manganese-oxidizing bacteria (preservation number CCTCC NO: M2012084) that had grown to the logarithmic phase, and the Place the flask in a 28°C 150r / min shaker for 10 days, centrifuge at 8000rpm for 5 minutes to collect the precipitate, wash the residual ions on the surface with ultrapure water, freeze-dry in a freeze dryer, and grind to obtain microbial secondary manganese oxide for use. The medium formula is as follows: yeast extract 0.5g, peptone 2g, agar 15g, artificial seawater (artificial seawater: NaCl13.14g, KCl0.56g, MgSO 4 .7H 2 O9.24g, CaCl 2 0.83g, add distilled water ...

Embodiment 2

[0023] Embodiment 2: the application of a kind of microorganism-based manganese-carbon composite material in dye wastewater treatment, its application process is:

[0024] Get each 0.1g of the microorganism-based manganese-carbon composite material and commercial activated carbon obtained in Example 1, add 100ml500mg / l sodium indigo disulfonate respectively, shake, centrifuge after 5-240min, measure the concentration of dyestuff in the supernatant , according to the concentration difference of the dye before and after the treatment, the removal amount of the microbial-based manganese-carbon composites for the dye wastewater was calculated. The experimental results showed that the removal of sodium indigo disulfonate by microbial-based manganese-carbon composites and commercial activated carbon was 471 mg / g and 59 mg / g, respectively, within 60 minutes, the former being about 10 times that of the latter.

Embodiment 3

[0025] Embodiment 3: a kind of preparation method of microorganism-based manganese-carbon composite material, its preparation steps are:

[0026] Add a certain amount of filter-sterilized HEPES buffer (pH 6-7) and 1ml 1mol / l MnCl under sterile conditions 2 Place it in the culture medium so that the final concentration is 10mmol / l. At the same time, insert the manganese oxidizing bacteria solution that has grown to the logarithmic phase with 1% inoculum, and put the triangular flask into a shaker at 28°C and 150r / min for cultivation. On 10 days, centrifuge at 8000 rpm for 5 minutes to collect the precipitate, wash the residual ions on the surface with ultrapure water, freeze-dry in a freeze dryer, and grind to obtain microbial secondary manganese oxide for later use.

[0027] Take 1g of the microbial secondary manganese oxide material prepared in the previous step, mix it with the activator (NaOH) at a mass ratio of 1:4, add 3ml-5ml deionized water and stir, completely immerse ...

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Abstract

The invention discloses a preparation method and an application of a microbial manganese-carbon composite material. The preparation method comprises the following steps of: (1) adding HEPES (hydroxyethyl piperazine ethanesulfonic acid) buffering solution for filtering and sterilizing and MnCl2 into a sterilized culture medium under the aseptic condition, inoculating the culture medium with a manganese oxidizing bacterial solution which grows to the logarithmic phase at an inoculum size of 1%, putting a triangular flask into a swing bed, culturing, centrifuging, collecting a precipitate, removing residual ions on the surface by washing with ultrapure water, and drying; (2) weighing the materials prepared in step (1), mixing the materials with sodium hydroxide serving as an activation agent according to a certain proportion, adding deionized water, stirring so that the materials are completely immersed in the activation agent, drying with a drying box, activating at high temperature, washing with hydrochloric acid until no bubble is generated, washing with deionized water until the raw materials are neutral, drying, and grinding to obtain the composite material. The preparation method of the composite material is practical, is convenient to operate and is suitable for the large-scale production of the composite material. The application of the composite material to the removal of indigo carmine in dyeing waste water finds that the composite material is high in adsorption speed and efficiency and can be widely applied to environmental governance.

Description

technical field [0001] The invention relates to the field of materials and environment, in particular to a preparation method of a microbial-based manganese-carbon composite material, and also relates to an application of a microbial-based manganese-carbon composite material. technical background [0002] Manganese oxide has adsorption, oxidation and catalytic oxidation capabilities, can adsorb and oxidize heavy metals, and can adsorb and oxidize organic pollutants. It is an ideal environmental pollution control material. Porous carbon is usually prepared by activation of coal such as bituminous coal, petroleum such as petroleum asphalt, synthetic polymers such as plastic rubber, plants such as wood, and carbon-containing materials such as urban sludge. Porous carbon has developed pore structure, high specific surface area and excellent adsorption performance, so it can adsorb a large number of inorganic and organic pollutants, and is widely used in pharmaceutical raw materi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/20B01J20/30C02F1/28C02F1/58C02F103/30
Inventor 廖水姣王革娇陈霄
Owner HUAZHONG AGRI UNIV
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