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Method for preparing mold oxide by mold fermentation biosynthesis

A technology of mold oxidation and fermentation organisms, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of unreasonable amino acid ratio and achieve the effect of reducing costs

Active Publication Date: 2015-08-12
SHANDONG TAIHUA BIO & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the above deficiencies, the present invention provides a method for preparing mold oxides by mold fermentation and biosynthesis, which is an improvement for the deficiencies in the prior art. It uses nitrogen source II to make up for the low protein content and amino acid ratio Unreasonable biological fermentation process; it is a major breakthrough in greatly improving product conversion rate and yield index under the premise of ensuring the quality of existing products, which cannot be achieved by various improvements over the years

Method used

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  • Method for preparing mold oxide by mold fermentation biosynthesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of mold fermentation biosynthesis prepares the technique of mold oxide, it comprises the following steps:

[0030] 1. Mycelia preparation:

[0031] (1) Add 60kg of glucose, 40kg of corn steep liquor; nitrogen source II (the final concentration in the medium of each component of nitrogen source II is: peptone: 5‰, yeast extract: 1‰, (NH 4 ) 2 SO 4 : 0.5‰, FeSO 4 ·7H 2 O: 0.01g‰, NaCl: 0.1‰, KH 2 PO 4 : 1‰, MgSO 4 ·7H 2 O: 0.2‰, Tween 80 1%); silkworm chrysalis powder 10kg; ammonium sulfate 5kg; polyether 1000mL was put into the primary fermentation tank at one time, 1.5 tons of water was added, the pH value was adjusted to 2, steam sterilized, and the temperature was lowered to 27 At ±3°C, insert 3 liters of spore suspension of Rhizopus niger, the concentration of spore suspension is 100,000 / mL, feed 0.21 times volume / min of air, stir and cultivate for 24 hours, and microscopically check, it is found that after the mycelia grow and mature, Move into the...

Embodiment 2

[0037] A kind of mold fermentation biosynthesis prepares the technique of mold oxide, it comprises the following steps:

[0038] 1. Mycelia culture:

[0039] (1) Combine 85kg of glucose, 57kg of corn steep liquor; nitrogen source II (the final concentrations in the medium of each component of nitrogen source II are: peptone: 5‰, yeast extract: 1‰, (NH 4 ) 2 SO 4 : 0.5‰, FeSO 4 ·7H 2 O: 0.01g‰, NaCl: 0.1‰, KH 2 PO 4 : 1‰, MgSO 4 ·7H 2 O: 0.2‰, Tween 80 1%); silkworm chrysalis powder 20kg; ammonium sulfate 10kg; polyether 2000mL was put into the primary fermentation tank at one time, added 1 ton of water, adjusted the pH value to 3.5, steam sterilized, and cooled to 27 At ±3°C, insert 28 liters of the spore suspension of Rhizopus niger, the concentration of the spore suspension is 1.5 million / mL, feed 0.24 times the volume / min of air, stir and cultivate for 36 hours, and microscopically check, it is found that after the mycelium grows mature, Move into the secondary f...

Embodiment 3

[0045] A kind of mold fermentation biosynthesis prepares the technique of mold oxide, it comprises the following steps:

[0046] 1. Mycelia culture:

[0047] (1) Add 75kg of glucose, 50kg of corn steep liquor; nitrogen source II (the final concentration in the medium of each component of nitrogen source II is: peptone: 5‰, yeast extract: 1‰, (NH 4 ) 2 SO 4 : 0.5‰, FeSO 4 ·7H 2 O: 0.01g‰, NaCl: 0.1‰, KH 2 PO 4 : 1‰, MgSO 4 ·7H 2 O: 0.2‰, Tween 80 1%); silkworm chrysalis powder 15kg; ammonium sulfate 8kg; polyether 1500mL was put into the primary fermentation tank at one time, added 1 ton of water, adjusted the pH value to 5, steam sterilized, and cooled to 27 At ±3°C, insert 50 liters of the spore suspension of Rhizopus niger purchased from the strain bank (purchased from the strain bank of China Institute of Pharmaceutical and Biological Products), the concentration of the spore suspension is 3 million / mL, and feed 0.28 times Volume / min air, stirring and culturing f...

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Abstract

The invention discloses a process for synthesizing and preparing mildew oxide by steroid hormone drug mildew fermentation and a needed novel nitrogen source. The process for synthesizing and preparing mildew oxide by mildew fermentation organisms comprises the following steps of: stirring and reacting under 24-30 DEG C by matching with the novel nitrogen source; ventilating in a biological fermentation process; carrying out oxidation reaction for 24-72 hours; filtering by a plate frame in a pressed manner for dehydrating and drying; converting substrate W-oxide into mycelium containing the mildew oxide; extracting by acetone to obtain a mildew oxide crude product; refining by a mixed solvent of toluene and chloroform to obtain the mildew oxide fine product. The formula of the novel nitrogen source is more suitable for the biological fermentation process requirements, so that the yield is 92% or higher, the product stability is stable, the yield is high, the indexes associated with the conversion rate and the yield are high and the economic benefits are considerable, and therefore, the process is suitable for the industrial production.

Description

technical field [0001] The invention relates to the field of mold fermentation of steroid hormone drugs, in particular to a method for preparing mold oxides through mold fermentation biosynthesis. Background technique [0002] Epoxy progesterone is white crystalline powder, odorless, soluble in methanol, toluene and other organic solvents. It is produced from natural steroidal diosgenin through ring-opening, acetylation, oxidation, hydrolysis, elimination, epoxidation, oxidation and other reactions. It is cheap and easy to get. It is a very important class of steroid drug intermediates that are used in the largest amount in my country. It is widely used in the synthesis of adrenocortical hormone drugs, and is the main raw material of steroid hormone drugs such as hydrocortisone, cortisone, prednisone, dexamethasone and fluocinolone. [0003] In 1952, Murray et al. of U.S. General Pharmaceutical Company used Rhizopus niger to convert progesterone into 11a-hydroxyprogesterone ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/845
Inventor 洪祥碧李艳芳
Owner SHANDONG TAIHUA BIO & TECH