Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03

A technology of MXB-03, octanoyl bromoxynil, applied in the field of biological high, can solve the problems of fish survival threat, rodent reproductive obstacle, strong toxicity, etc., achieve good removal effect, solve excessive pesticide residues and herbicides The problem of poisoning and the effect of increasing added value

Active Publication Date: 2014-08-27
中持(江苏)环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Bromoxynil can cause reproductive disorders in rodents. Once it enters the water, it will produce strong toxicity and pose a threat to the survival of fish

Method used

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  • Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03
  • Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03
  • Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening and isolation of bromoxynil-degrading Pseudomonas MXB-03

[0027] Get the activated sludge 5.0ml that obtains from the waste water treatment tank of the production workshop of a certain pesticide factory that produces bromoxynil octanoyl and add bromoxynil octanoyl concentration and be 100ml inorganic salt liquid culture of 100mg / L, its formula is: Liter contains 1.50 g K 2 HPO 4 , 0.50 g KH 2 PO 4 , 0.20 g MgSO 4 . 7H 2 O, 1.00 g NaCl, 1.00 g (NH 4 ) 2 SO 4 , pH 7.0, cultured with shaking at 160r / min, 30°C, transferred to fresh inorganic salt medium at 5% inoculum every 5 days, and transferred 5 times in a row.

[0028] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enri...

Embodiment 2

[0030] Example 2 Shake flask degradation experiment of Pseudomonas MXB-03

[0031] In the inorganic salt medium containing 100 mg / L bromoxynil octanoyl, the formulation is: 1.50 g K per liter 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 . 7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , pH 7.0, inoculate MXB-03 at 5% inoculum size, culture with shaking at 30°C, and sample for measurement after 72 hours. Such as image 3 with Figure 4 As shown, compared with the blank, the absorption peak of the treated sample decreased significantly, and bromoxynil was degraded by more than 90%.

Embodiment 3

[0032] Embodiment 3 Contains the preparation of Pseudomonas MXB-03 bacterial agent

[0033] The original species of bromoxynil-octanoyl degrading Pseudomonas MXB-03 screened and isolated in Example 1 was activated on a petri dish, and the degradation performance was measured, and inoculated on the inclined surface of the test tube for later use. The test tube was inoculated in a 1000ml shake flask containing 200ml LB medium. The formula of LB medium was: yeast extract 5.00g / L, peptone 10.00g / L, NaCl 10.00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase , ready to inoculate the seed pot. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask str...

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Abstract

The invention provides a degrading bacterial agent for eliminating bromoxynil octanoyl residue in waste water, which belongs to the field of biological high technology. The strain used was Gram-negative bacteria MXB-03, which was identified as Pseudomonas sp. The main biological characteristic is G-. The colony on LB solid medium is round, with a diameter of about 2 mm, light yellow, protruding surface, smooth, and edges. After staining with crystal violet, the bacteria are short rod-shaped under the microscope without spores. Positive flagella; positive for catalase; negative for oxidase; unable to hydrolyze starch and liquefy gelatin, the bacteria can also grow on octanoyl bromoxynil as the sole carbon source. The direct application of degrading bacteria products can reduce the amount of pesticide residues in water by more than 90%.

Description

technical field [0001] The invention belongs to the field of biological high technology, uses microorganisms to degrade chemical pesticides, is suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production, and specifically relates to a strain of octanoylbromoxynil-degrading pseudomonas MXB-03 and its application. Background technique [0002] With the improvement of people's living standards and the enhancement of environmental protection awareness, the whole society is paying more and more attention to the direct harm and potential impact of pesticide residues. People urgently need clean and green agricultural products without pesticide residue pollution. However, judging from the current form of agricultural production and population status in our country, the pollution-free production model at the cost of stopping the use of pesticides and reducing agricultural output is not feasible. Pesticides will contin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/38A62D101/04A62D101/28
Inventor 顾乔梅张金张永乐蔡双梅
Owner 中持(江苏)环境建设有限公司
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