Improved radix sophorae flavescentis thin-layer chromatography identification method

A technology of thin-layer chromatography and sophora flavescens, which is applied in the field of authenticity identification of drugs, can solve the problems of low dissolution rate of index components, difficulty in controlling dosage, excessive decolorization, etc., achieve uniform and clear color of spots, easy to observe, and increase the degree of separation Effect

Active Publication Date: 2013-08-14
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The above-mentioned TLC identification method judges the authenticity of Sophora flavescens by identifying the index components matrine, oxymatrine and sophoridine of Sophora flavescens, and there are the following problems: (1) The preparation of the test solution adopts the soaking method, It takes a long time (about 12 hours), and the dissolution rate of the index components is low; (2) TLC identification requires two TLC plates, three developing agents, two developing agents, three developing operations and Four color development operations, and the preparation of the two developing agents needs to be placed below 10

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  • Improved radix sophorae flavescentis thin-layer chromatography identification method

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Embodiment 1

[0021] Example 1. Using the improved Sophora flavescens TLC identification method of the present invention to identify Sophora flavescens

[0022] The improved Sophora flavescens TLC identification method of the present invention includes the following steps:

[0023] a. Preparation of reference substance solution: Take matrine reference substance, oxymatrine reference substance and sophoridine reference substance, and add ethanol to make a solution containing 0.2mg per 1ml as reference substance solution;

[0024] b. Preparation of test solution: Take 1g of Sophora flavescens test substance powder, add 0.3ml of concentrated ammonia test solution, 25ml of chloroform, 250W ultrasonic for 20 minutes, filter, evaporate the filtrate to dryness, and add 1ml of chloroform to the residue to dissolve it. Test solution;

[0025] c. Development and color development: draw 4μl each of the three reference solution prepared in step a and the test solution prepared in step b, and place them on t...

Embodiment 2

[0028] Example 2. Investigation on the system suitability of the improved Sophora flavescens TLC identification method of the present invention

[0029] 1. Repeatability

[0030] Take three samples of Sophora flavescens from different batches, and test them according to the improved method for identification of Sophora flavescens by thin layer chromatography described in Example 1. Each sample of Sophora flavescens is subjected to three repeated tests, and 9 test results are evaluated , To examine the repeatability of the method.

[0031] The test results showed that in the 9 test product chromatograms, the same orange spots were displayed at the positions corresponding to the reference product chromatograms, and the spot colors were uniform and clear, and contrasted sharply with the background color without obvious tailing. In the chromatogram of the test product, the ratio shift value and separation degree of matrine component, oxymatrine component and sophoridine component are sh...

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Abstract

The invention discloses an improved radix sophorae flavescentis thin-layer chromatography identification method. In comparison with the thin-layer chromatography identification method specified under a radix sophorae flavescentis item of Chinese Pharmacopoeia 2010, the method has been improved in the following aspects: (1) the test solution is prepared by an ultrasonic method from a soaking method; the amount of thin layer plates is reduced to be one from two, and three components of sophocarpidine, oxymatrine and sophoridine are unfolded on the same thin layer plate; (3) the amount of developing solvents is reduced to be two from three, that is methylbenzene-acetone-ethanol-ammonia water (6:8:2:0.28), methanol-chloroform (1.6:10); and (4) developing by a single color agent: bismuth potassium iodide-0.6mol/L hydrochloric acid (1:1). The method is simple to operate, time-saving, low in cost and good in separation effect and developing effect, the result can be easily judged and the color agent can be stored for a long time. A system suitability study proves that the method has favorable repeatability, sensitivity and developing stability, and is suitable for radix sophorae flavescentis thin-layer chromatography identification.

Description

Technical field [0001] The invention belongs to the technical field of medicine analysis, and relates to a method for authenticating medicines. Background technique [0002] Sophora flavescens is the dried root of the leguminous plant Sophora flavescens Ait.; bitter taste, cold in nature; Guixin, liver, stomach, large intestine, bladder meridian; has the effects of clearing heat and dampness, killing insects and diuresis; used for heat dysentery, Hematochezia, jaundice, closed urine, leucorrhea, vaginal swelling and itching, eczema, eczema, skin itching, scabies, leprosy, external treatment of trichomonas vaginitis. In recent years, with the gradual increase in market consumption of Sophora flavescens, its price continues to rise, and there have been many phenomena of shoddy and fake products, which not only reduces the quality of medicines, but also has a great negative impact on the Chinese medicinal material market. [0003] The 2010 edition of the Chinese Pharmacopoeia stipula...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 刘娟罗艺晨凌榕镔宋旭琴辛良
Owner SOUTHWEST UNIVERSITY
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