Improved radix sophorae flavescentis thin-layer chromatography identification method

A technology of thin-layer chromatography and sophora flavescens, which is applied in the field of authenticity identification of drugs, can solve the problems of low dissolution rate of index components, difficulty in controlling dosage, excessive decolorization, etc., achieve uniform and clear color of spots, easy to observe, and increase the degree of separation Effect
CN103245754AActive Publication Date: 2013-08-14SOUTHWEST UNIVERSITY
4 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2013-08-14

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an improved radix sophorae flavescentis thin-layer chromatography identification method. In comparison with the thin-layer chromatography identification method specified under a radix sophorae flavescentis item of Chinese Pharmacopoeia 2010, the method has been improved in the following aspects: (1) the test solution is prepared by an ultrasonic method from a soaking method; the amount of thin layer plates is reduced to be one from two, and three components of sophocarpidine, oxymatrine and sophoridine are unfolded on the same thin layer plate; (3) the amount of developing solvents is reduced to be two from three, that is methylbenzene-acetone-ethanol-ammonia water (6:8:2:0.28), methanol-chloroform (1.6:10); and (4) developing by a single color agent: bismuth potassium iodide-0.6mol / L hydrochloric acid (1:1). The method is simple to operate, time-saving, low in cost and good in separation effect and developing effect, the result can be easily judged and the color agent can be stored for a long time. A system suitability study proves that the method has favorable repeatability, sensitivity and developing stability, and is suitable for radix sophorae flavescentis thin-layer chromatography identification.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention belongs to the technical field of medicine analysis, and relates to a method for authenticating medicines. Background technique

[0002] Sophora flavescens is the dried root of the leguminous plant Sophora flavescens Ait.; bitter taste, cold in nature; Guixin, liver, stomach, large intestine, bladder meridian; has the effects of clearing heat and dampness, killing insects and diuresis; used for heat dysentery, Hematochezia, jaundice, closed urine, leucorrhea, vaginal swelling and itching, eczema, eczema, skin itching, scabies, leprosy, external treatment of trichomonas vaginitis. In recent years, with the gradual increase in market consumption of Sophora flavescens, its price continues to rise, and there have been many phenomena of shoddy and fake products, which not only reduces the quality of medicines, but also has a great negative impact on the Chinese medicinal material market.

[0003] The 2010 edition of the Chinese Pharmacopoeia stipula...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More