Improved radix sophorae flavescentis thin-layer chromatography identification method

Abstract

The invention discloses an improved radix sophorae flavescentis thin-layer chromatography identification method. In comparison with the thin-layer chromatography identification method specified under a radix sophorae flavescentis item of Chinese Pharmacopoeia 2010, the method has been improved in the following aspects: (1) the test solution is prepared by an ultrasonic method from a soaking method; the amount of thin layer plates is reduced to be one from two, and three components of sophocarpidine, oxymatrine and sophoridine are unfolded on the same thin layer plate; (3) the amount of developing solvents is reduced to be two from three, that is methylbenzene-acetone-ethanol-ammonia water (6:8:2:0.28), methanol-chloroform (1.6:10); and (4) developing by a single color agent: bismuth potassium iodide-0.6mol / L hydrochloric acid (1:1). The method is simple to operate, time-saving, low in cost and good in separation effect and developing effect, the result can be easily judged and the color agent can be stored for a long time. A system suitability study proves that the method has favorable repeatability, sensitivity and developing stability, and is suitable for radix sophorae flavescentis thin-layer chromatography identification.

Description

Technical field [0001] The invention belongs to the technical field of medicine analysis, and relates to a method for authenticating medicines. Background technique [0002] Sophora flavescens is the dried root of the leguminous plant Sophora flavescens Ait.; bitter taste, cold in nature; Guixin, liver, stomach, large intestine, bladder meridian; has the effects of clearing heat and dampness, killing insects and diuresis; used for heat dysentery, Hematochezia, jaundice, closed urine, leucorrhea, vaginal swelling and itching, eczema, eczema, skin itching, scabies, leprosy, external treatment of trichomonas vaginitis. In recent years, with the gradual increase in market consumption of Sophora flavescens, its price continues to rise, and there have been many phenomena of shoddy and fake products, which not only reduces the quality of medicines, but also has a great negative impact on the Chinese medicinal material market. [0003] The 2010 edition of the Chinese Pharmacopoeia stipula...

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Problems solved by technology

[0004] The above-mentioned TLC identification method judges the authenticity of Sophora flavescens by identifying the index components matrine, oxymatrine and sophoridine of Sophora flavescens, and there are the following problems: (1) The preparation of the test solution adopts the soaking method, It takes a long time (about 12 hours), and the dissolution rate of the index components is low; (2) TLC identification requires two TLC plates, three developing agents, two developing agents, three developing operations and Four color development operations, and the preparation of the two developing agents needs to be placed below 10°C. The chromogenic agent bismuth potassium iodide test solution is prone to precipitation when stored at room temperature, and needs to be prepared and used immediately. The whole process is cumbersome and time-consuming; (3) The principle of color development of this method is actually to use bismuth potassium iodide test solution for color development first, and then use sodium nitrite ethanol test solution for decolorization. It is difficult to control the dosage during color development and decolorization, and it is easy to cause excessive color development. Deep or excessive bleaching, affecting the judgment of the result

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