Total protein detection reagent

A technology for detecting reagents and total protein, applied in the field of medical testing, can solve the problems of lack of testing professionals and insufficient automatic biochemical testing equipment and facilities, and achieve the effects of good sensitivity, low price and easy operation

Active Publication Date: 2013-09-04
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Components of the diluent:

[0036] Tris (pH6.5-7.5): 0.5mol / L

[0037] Triton X-100: 5.0%

[0038] Ethylparaben: 5.0%

[0039] Potassium ferricyanide: 5mmol / L

[0040] Vitamin C oxidase: 50KU / L

[0041] Reagent components:

[0042] Sodium hydroxide: 200mmol / L

[0043] Copper sulfate: 200mmol / L

[0044] Sodium potassium tartrate: 50mmol / L

[0045] Potassium iodide: 50mmol / L

[0046] BSA: 5.0g / L.

Embodiment 2

[0048] Components of the diluent:

[0049] Tris (pH6.5-7.5): 1.0mol / L

[0050] Brij-35: 10.0%

[0051] proclin300: 10.0%

[0052] Bilirubin oxidase: 100KU / L

[0053] Vitamin C oxidase: 100KU / L

[0054] Reagent components:

[0055] Sodium hydroxide: 500mmol / L

[0056] Copper sulfate: 500mmol / L

[0057] Sodium potassium tartrate: 100mmol / L

[0058] Potassium iodide: 100mmol / L

[0059] Glycerin: 10g / L.

Embodiment 3

[0061] Components of the diluent:

[0062] Tris (pH6.5-7.5): 0.01mol / L

[0063] PEG6000: 0.1-10.0%

[0064] Methylparaben: 0.1-10.0%

[0065] Potassium ferrocyanide: 1mmol / L

[0066] Vitamin C oxidase: 1KU / L

[0067] Reagent components:

[0068] Sodium hydroxide: 0.5mmol / L

[0069] Copper sulfate: 0.5mmol / L

[0070] Potassium sodium tartrate: 0.1mmol / L

[0071] Potassium iodide: 0.1mmol / L

[0072] Trehalose: 0.1g / L.

[0073] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0074] 1. Precision

[0075] Table 1. Precision evaluation results

[0076]

[0077]

[0078] 2. Linear

[0079] The standard serum sample is added with different concentrations of analytes for detection, and the obtained TP linearity results are shown in figure 1 .

[0080] 3. Methodological comparison test

[0081] Compared with the TP result value determined on the automatic biochemical analyzer, the resu...

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Abstract

The invention discloses a total protein detection reagent which comprises a diluent and a reaction reagent, wherein the diluent is composed of trihydroxymethyl aminomethane, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of sodium hydroxide, copper sulfate, potassium sodium tartrate, potassium iodide and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a total protein detection reagent for a POCT analyzer. Background technique [0002] Total protein (TP) is an important index to detect the metabolic capacity of liver function, reflecting the reserve capacity of the liver. High TP will bring certain harm to people. Generally, the TP of normal people is between 60-80g / L. If it exceeds this range, it means that the liver has been damaged to a certain extent. [0003] At present, TP is generally detected by various large-scale automatic biochemical analyzers. However, due to the high price of large-scale automatic biochemical analyzers and complicated operations, operators need to have relevant professional knowledge and receive corresponding training, and the requirements for supporting conditions are high. It needs to be equipped with a regulated power supply, a pure water machine, etc., and it occupies a large area, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 邹炳德邹继华张桂春沃燕波周海滨
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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