mldh-fluorescein intercalation assembled fluorescent probe

A fluorescent probe, assembly-type technology, applied in fluorescence/phosphorescence, luminescent materials, chemical instruments and methods, etc., can solve problems such as no literature reports, and achieve the effects of enhanced optical stability, bright fluorescent signal, and easy operation.

Inactive Publication Date: 2015-09-09
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people have studied the intercalation effect and fluorescence properties of fluorescent yellow anion on ZnAlLDH, but there is no literature report on the fluorescent labeling of magnetic layered double hydroxides (MLDH) delivery system.

Method used

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  • mldh-fluorescein intercalation assembled fluorescent probe
  • mldh-fluorescein intercalation assembled fluorescent probe
  • mldh-fluorescein intercalation assembled fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 The MLDH-Fluorescein fluorescent complex was synthesized by ion exchange method, and the product crop phase was characterized and analyzed. The specific operation process is as follows:

[0023] Constant temperature at 0-60°C (preferably 40°C), N 2 Or Ar protection and 300 r·min -1 Dissolve 61.63 g of FeCl in 300 mL of double distilled water under magnetic stirring 2 4H 2 O, 41.91 g FeCl 3 ·6H 2 O, then add 500 mL 2 mol·L dropwise -1 NaOH solution, control the pH of the co-precipitation end point to 6.0-8.0 (preferably 6.50). After the obtained slurry was crystallized in situ for 30-45 min under the same conditions as above, the heating and magnetic stirring system were turned off, and the temperature of the water bath was lowered. Use absolute ethanol at -80°C to -20°C for solvent conversion, and after the solid phase settles, in N 2 Protection, constant temperature at -10°C and 5000 r min -1 Centrifuge for 10 min under the condition of rotating...

Embodiment 2

[0026] Example 2 The MLDH-FLU fluorescent probe was synthesized by the ion exchange method, and the cell internalization effect of MLDH-FLU was examined by laser confocal fluorescence microscopy. The specific operation process was as follows:

[0027] The preparation of the MLDH carrier is the same as in Example 1.

[0028] Another 500 mL reactor was equipped with pH electrode, N 2Drive the system, set a constant temperature condition of -4°C to 10°C through the salt, ice, water and heating system (10°C is preferred in this example). Add 0.0188 mol MLDH to the reactor, and disperse evenly with 100 mL distilled water; then, weigh 6.25 g (0.0188 mol) fluorescent yellow, moisten it with a small amount of water and add concentrated NaOH dropwise to dissolve, add water to dilute to 400 mL, and adjust the pH to 7.45 after adding to the reactor, in N 2 The reaction was carried out for 90 min under driving, constant temperature and magnetic stirring conditions. Take out the sl...

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Abstract

The invention relates to an MLDH (magnetic layered double hydroxides)-Fluorescein intercalation assembled type fluorescent probe. The invention is characterized in that the MLDH-Fluorescein intercalation assembled type fluorescent probe is prepared by a low-temperature ion exchange reaction, with MLDH as carriers and fluorescein as an intercalation object. The synthesized MLDH-Fluorescein probe has superparamagnetism, bright fluorescence signals, high thermal stability, strong nuclear targeting property in cell transmission, and capability of quickly infiltrating a cell membrane and passing through cytoplasm to deliver a fluorescent material to cell nucleus and enrich the fluorescent material to a nucleolus, so that the MLDH-Fluorescein probe can be used as a fluorescent probe for researching a cell transport mechanism of an MLDH system and evaluating the nuclear targeting transmission efficiency. The MLDH-Fluorescein intercalation assembled type fluorescent probe is applicable to establishment of a fluorescein, fluorandiol marked medicament or biomolecular targeting transport system and has the advantages of bright fluorescence signals, high storage stability, integration of multiple functions, simplicity in operation, low cost and the like.

Description

technical field [0001] The invention belongs to the category of biomedical functional materials, is related to the targeted delivery and tracing technology of drugs and biomolecules, and specifically relates to an MLDH-Fluorescein intercalation assembly type fluorescent probe. Background technique [0002] In the field of biomedicine, layered double hydroxides (Layered double hydroxides, LDH) can be used to carry some biomolecules and specific drugs, with excellent cell transport and controlled release performance. Fluorescein isothiocyanate (Flourescein iso-thiocyanate, FITC) through side groups and biomolecules containing amines and sulfhydryls through nucleophilic reactions to form a valence bond, as a fluorescent dye is widely used in protein and antibody labeling, with LDH in the biomedical field With the in-depth application research, people have done a lot of work on the fluorescent labeling of magnesium, zinc, and aluminum-based LDH by FITC. The probe function of FI...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/64
Inventor 苟国敬孙岳张逢源闫乾顺曹菊琴许红平
Owner NINGXIA MEDICAL UNIV
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