Applications of macrolide compound in preventing plant downy mildew

A compound and downy mildew technology, applied in application, plant growth regulator, botanical equipment and methods, etc., can solve problems such as difficult decomposition, weakened drug effect, and drug loss

Active Publication Date: 2013-09-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Because traditional chemical synthetic agents are easy to cause pathogenic bacteria to develop drug resistance, long-term use will cause the drug effect to weaken, and it is not easy to decompose in the environment, and the res

Method used

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  • Applications of macrolide compound in preventing plant downy mildew
  • Applications of macrolide compound in preventing plant downy mildew
  • Applications of macrolide compound in preventing plant downy mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of the compound represented by formula (1) of the present invention

[0022] Paecilomyces sp.SC0924CGMCC No.2900 strain was inoculated on 11.50L rice solid medium (rice and water with a mass ratio of 1:1), and cultured statically at 26°C for 22 days in the dark to obtain a solid fermentation culture. The obtained solid fermentation culture was extracted 3 times with 1.5 times the volume of 95% ethanol for 24 hours each time. After the ethanol aqueous solution was concentrated under reduced pressure to remove ethanol, it was extracted 3 times with an equal volume of chloroform, and the extract was concentrated to obtain a chloroform extract 97.50 g.

[0023] The chloroform extract was subjected to silica gel column chromatography, and the chloroform-methanol mixed solvent with a volume ratio of 100:0 to 80:20 was used for gradient elution, and the similar fractions were detected by thin-layer chromatography, and petroleum ether was collected...

Embodiment 2

[0026] Embodiment 2: The in vitro inhibitory activity experiment of the Hypothemycin obtained in Example 1 to the common pathogens of plant downy mildew --- litchi downy mildew, Pythium melon and fruit, Phytophthora melon and Phytophthora tabacum

[0027] The Pythium aphanidermatum used in the experiment was purchased from the China Agricultural Microorganism Culture Collection and Management Center, and the Peronophythora litchii, Phytophthora melonis and Phytophthora nicotianae were provided by Dr. Pan Ruqian, School of Resources and Environment, South China Agricultural University.

[0028]The anti-downy mildew activity of the compound Hypothemycin was determined by the filter paper agar diffusion method: four kinds of downy mildew pathogens including activated Phytophthora litchii, Pythium melon and fruit, Phytophthora melon and Phytophthora nicotianae were inoculated into In the petri dish with PDA solid medium, use a T-shaped applicator to evenly coat it for later use; th...

Embodiment 3

[0030] Embodiment 3: The Hypothemycin that embodiment 1 obtains is to the inhibitory activity experiment of downy mildew in litchi storage after harvesting

[0031] Test sample: the Hypothemycin obtained in Example 1 was dissolved in a small amount of ethanol, diluted with deionized water to 100, 200, and 300 μg / mL, and the final concentration of ethanol was 2.5% by volume.

[0032] Test bacteria liquid: Pythora lychee was cultured in PDA medium for 3 days, adding deionized water to wash the surface spores, counting them under a microscope and diluting them into a bacteria liquid containing about 106 spores per mL for use.

[0033] Litchi for testing: Litchi variety "Huaizhi" (Litchi chinesis Sonn.cv. Huaizhi) was collected from Conghua City, Guangdong Province. After harvesting, it was transported back to the laboratory immediately, and the processing began within 4 hours. Select fruits with the same size, no pests and diseases, and a maturity of about 8.5% (most of the peel ...

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Abstract

The invention relates to applications of Hypothemycin, shown in a formula (1) described in the specification, in preventing downy mildew of plant and harvested fruits. Experiments prove that the Hypothemycin can obviously inhibit the growth of peronophythora litchi and phytophthora nicotianae, and has certain inhibition activity to pythium aphanidermatum and phytophthora melonis; and in addition, the Hypothemycin has an obvious inhibition effect on the downy blight infection of the harvested litchi fruits, as well as an obvious inhibition effect on the rotting of harvested litchis.

Description

technical field [0001] The invention relates to a new application of a macrocyclic lactone compound Hypothemycin, in particular to its new application in the prevention and treatment of downy mildew of plants and post-harvest fruits. Background technique [0002] Plant downy mildew usually refers to plant diseases caused by pathogens such as Pythium, Peronospora, Phytophthora and Peronospora of Oomycetes Peronospora, one of its important characteristics is that it produces obvious and visible light when the disease is severe or the humidity is appropriate. Downy mildew (ie the sporangia and ascospores of the pathogen). Among them, the ones that are widely popular in China include Pythium melon, Phytophthora melon, and Phytophthora lychee. [0003] Pythium aphanidermatum is an important plant pathogenic fungus, which is widely distributed in the world. It often causes rot and damping of various crops, and often causes spoilage and deterioration of fruits and vegetables after...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00
Inventor 徐良雄段学武魏孝义薛璟花吴萍
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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