Application of Lysinibacillus sp Cr-6

A lysinic bacillus, cu2 technology, applied in the restoration of contaminated soil, water pollutants, water/sludge/sewage treatment, etc., can solve problems such as genetic defects, limited adsorption capacity, skin sensitivity, etc. , to achieve the effect of rapid growth, short time required and complete restoration

Inactive Publication Date: 2013-11-20
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hexavalent chromium is an ingestion poison / inhalation extremely poison, skin contact may cause sensitivity; it is more likely to cause hereditary gene defects, inhalation may cause cancer, and has persistent danger to the environment
[0003] At present, the treatment of hexavalent chromium is mainly to reduce it to trivalent chromium which is less harmful to the human body. The commonly used treatment methods for hexavalent chromium in sewage include physical adsorption method and chemical reduction method. Physical adsorption method needs to provide adsorption materials, and The adsorption capacity is limited, and the chemical reduction method needs to add additional reducing chemical substances to the sewage, which is easy to cause secondary pollution and the cost is high
The remediation technologies of hexavalent chromium in soil mainly include immobilization / stabilization technology, chemical reduction technology, chemical leaching technology, electrodynamic remediation technology, phytoremediation technology, etc. These methods are time-consuming, expensive, and prone to secondary pollution. and other shortcomings

Method used

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  • Application of Lysinibacillus sp Cr-6
  • Application of Lysinibacillus sp Cr-6
  • Application of Lysinibacillus sp Cr-6

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Collect 20-40cm soil layer samples from Zhengzhou reclaimed water recharge site, wrap them in sterilized tin foil, bring them back to the example room quickly, and store them in a refrigerator at 4°C until use.

[0020] Weigh 10g of the above soil sample into a 250mL Erlenmeyer flask filled with 100mL of sterilized LB medium, add 1mL of sterilized potassium dichromate solution (hexavalent chromium concentration is 1000mg / L), place at 30°C, Shake culture at constant temperature at 100r / min for 48 hours, all items used have been sterilized at 121°C for 20 minutes, and all operations are completed on a sterile clean bench.

[0021] Let the soil suspension that has been cultivated for 48 hours stand for half an hour, draw 1mL to 9mL of sterile dilution water with a sterile pipette tip, and make a gradient dilution step by step according to this step to 10 -9 , take 10 respectively -6 ~10 -9 Put 0.1 mL of the diluted solution on the cooled beef extract-peptone medium plate...

Embodiment 2

[0028] The bacterial strain Cr-6 in Example 1 is carried out Gram staining embodiment, and adopt transmission electron microscope to observe cell shape, then observe the colony shape of bacterial plate growth, analyze the physiological and biochemical characteristics of bacteria, finally analyze its rDNA sequence, judge Strain Cr-6 is Bacillus lysinica.

[0029] A certain number of bacterial strains Cr-6 were inoculated in liquid medium and cultured under certain conditions. The growth curve was drawn with the growth time as the abscissa and the bacterial growth as the ordinate. In this example, the growth curves of the bacteria without adding hexavalent chromium and the initial hexavalent chromium concentration of 10 mg / L were drawn respectively, and the cell culture fluid cultured overnight for about 12 hours was inoculated to 250 mL of sterilized bacteria at a ratio of 4%. In the LB medium, after mixing, divide 20mL into a glass bottle with a volume of 100mL, shake culture ...

Embodiment 3

[0036] In this example, the influence of pH value on the reduction of hexavalent chromium was studied.

[0037] Inoculate 0.5 mL of the cell culture solution that was cultured overnight for about 12 hours into a 100 mL glass bottle filled with 20 mL of LB medium, in which the concentration of hexavalent chromium is 10 mg / L, and place it in a shaker at 30°C and 100 r / min. Culture, the sampling time is set to 0h, 2h, 4h, 6h, 9h, 12h, and the glass bottle is taken out at the corresponding sampling time point to measure the pH value of the culture solution and the bacterial optical density value A 600 And the concentration of hexavalent chromium in the culture solution was measured, and the reduction rate of hexavalent chromium was calculated. The initial pH values ​​were set at 5, 6, 7, 8, and 9, respectively. After the medium was prepared, it was adjusted to the corresponding pH values ​​with 10% sodium hydroxide solution and 10% hydrochloric acid, and then sterilized. Parallel...

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Abstract

The invention provides an application of Lysinibacillus sp Cr-6. The Lysinibacillus sp Cr-6 is used for reducing Cr6<+>, so as to reduce the Cr6<+> into Cr3<+>. In an anaerobic environment, the Lysinibacillus sp Cr-6 is used for reducing the Cr6<+> into Cr3<+>. When the pH value of the Lysinibacillus sp Cr-6 is 6-9, the Cr6<+> is reduced into Cr3<+>. Under the condition that Zn2<+> ions and Cu2<+> ions exist in the Lysinibacillus sp Cr-6, the Cr6<+> is reduced into Cr3<+>. The concentration of the Zn2<+> ions is 1-10mg/L; the concentration of the Cu2<+> ions is 1-20mg/L. According to the application of the Lysinibacillus sp Cr-6, the technical problem of reduction of hexavalent chromium in a natural environment is solved, so that a degrading bacterium for the reduction of the hexavalent chromium is provided. The Lysinibacillus sp Cr-6 is strong in survival ability, complete to degrade and short in required time; microorganisms are degraded completely. Thus, the Lysinibacillus sp Cr-6 has good social, ecological and economic benefits.

Description

technical field [0001] The invention relates to the application of a microorganism, in particular to the application of Lysinibacillus sp. Cr-6 in reducing hexavalent chromium ions, which belongs to the field of microorganism application. Background technique [0002] Hexavalent chromium is an ingestion poison / inhalation extremely poison, skin contact may cause sensitivity; it is more likely to cause hereditary gene defects, inhalation may cause cancer, and has a persistent danger to the environment. But these are the characteristics of hexavalent chromium, chromium metal, trivalent or tetravalent chromium do not have these toxicity. Hexavalent chromium is easily absorbed by the human body, and it can invade the human body through digestion, respiratory tract, skin and mucous membranes. It has been reported that when breathing air containing different concentrations of chromic anhydride, there are varying degrees of hoarseness, nasal mucosal atrophy, and in severe cases, na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34B09C1/10C02F101/22
Inventor 廉晶晶靳孟贵罗泽娇
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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