Application of Lysinibacillus sp Cr-6
A lysinic bacillus, cu2 technology, applied in the restoration of contaminated soil, water pollutants, water/sludge/sewage treatment, etc., can solve problems such as genetic defects, limited adsorption capacity, skin sensitivity, etc. , to achieve the effect of rapid growth, short time required and complete restoration
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Embodiment 1
[0019] Collect 20-40cm soil layer samples from Zhengzhou reclaimed water recharge site, wrap them in sterilized tin foil, bring them back to the example room quickly, and store them in a refrigerator at 4°C until use.
[0020] Weigh 10g of the above soil sample into a 250mL Erlenmeyer flask filled with 100mL of sterilized LB medium, add 1mL of sterilized potassium dichromate solution (hexavalent chromium concentration is 1000mg / L), place at 30°C, Shake culture at constant temperature at 100r / min for 48 hours, all items used have been sterilized at 121°C for 20 minutes, and all operations are completed on a sterile clean bench.
[0021] Let the soil suspension that has been cultivated for 48 hours stand for half an hour, draw 1mL to 9mL of sterile dilution water with a sterile pipette tip, and make a gradient dilution step by step according to this step to 10 -9 , take 10 respectively -6 ~10 -9 Put 0.1 mL of the diluted solution on the cooled beef extract-peptone medium plate...
Embodiment 2
[0028] The bacterial strain Cr-6 in Example 1 is carried out Gram staining embodiment, and adopt transmission electron microscope to observe cell shape, then observe the colony shape of bacterial plate growth, analyze the physiological and biochemical characteristics of bacteria, finally analyze its rDNA sequence, judge Strain Cr-6 is Bacillus lysinica.
[0029] A certain number of bacterial strains Cr-6 were inoculated in liquid medium and cultured under certain conditions. The growth curve was drawn with the growth time as the abscissa and the bacterial growth as the ordinate. In this example, the growth curves of the bacteria without adding hexavalent chromium and the initial hexavalent chromium concentration of 10 mg / L were drawn respectively, and the cell culture fluid cultured overnight for about 12 hours was inoculated to 250 mL of sterilized bacteria at a ratio of 4%. In the LB medium, after mixing, divide 20mL into a glass bottle with a volume of 100mL, shake culture ...
Embodiment 3
[0036] In this example, the influence of pH value on the reduction of hexavalent chromium was studied.
[0037] Inoculate 0.5 mL of the cell culture solution that was cultured overnight for about 12 hours into a 100 mL glass bottle filled with 20 mL of LB medium, in which the concentration of hexavalent chromium is 10 mg / L, and place it in a shaker at 30°C and 100 r / min. Culture, the sampling time is set to 0h, 2h, 4h, 6h, 9h, 12h, and the glass bottle is taken out at the corresponding sampling time point to measure the pH value of the culture solution and the bacterial optical density value A 600 And the concentration of hexavalent chromium in the culture solution was measured, and the reduction rate of hexavalent chromium was calculated. The initial pH values were set at 5, 6, 7, 8, and 9, respectively. After the medium was prepared, it was adjusted to the corresponding pH values with 10% sodium hydroxide solution and 10% hydrochloric acid, and then sterilized. Parallel...
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