Preparation method of lamb-dysentery clostridium perfringens type B toxoid vaccine

Abstract

The invention relates to a preparation method of a preparation method of a lamb-dysentery clostridium perfringens type B toxoid vaccine. The preparation method comprises the following steps of inoculating the clostridium perfringens type B in a thioglycollate fluid culture medium for enriched culture under the condition of an anaerobic environment at 38 DEG C for 14 h; adding the culture to a culture medium of tryptone, dextrin, yeast extract and L-arginine which are dissolved by a buffer solution, carrying out shaking culture in the anaerobic environment for 5 h, and then performing 3800 g centrifuging at 4 DEG C for 15 min, and filtering to remove bacteria in a Seitz filter; adding a toxoid inactivated previously to a sterile white oil adjuvant in the ratio of 1: 1, and adding 0.01% of merthiolate, thereby obtaining the toxoid vaccine. The vaccine is free of toxic and side effects, high in valence, strong in immunizing power, and capable of effectively preventing and controlling clostridium perfringens type B induced lamb dysentery and enterotoksemia or necroticenteritis of sheep, goat, foal and calf.

Description

(1) Technical field [0001] The invention relates to a preparation method of Clostridium perfringens type B toxoid vaccine for lamb dysentery. (2) Background of the invention [0002] Clostridium perfringens type B can cause dysentery in lambs, enterotoxemia or necrotic enteritis in sheep, goats, foals and calves, and the main pathogenic toxins are α, β, ε toxins. Necrotizing enteritis caused by beta-toxin is generally believed to be caused by trypsin in food preventing the breakdown of the toxin in the intestine, thereby causing the disease to occur. In addition, the toxin may also be a neurotoxin. The B-type ε-toxin has a small amount of secretion, which can promote the contraction of the aorta and other arteries in animals, resulting in increased blood pressure; in addition, it also has the ability to bind to vascular epithelial receptors in the blood-brain barrier, thereby causing vascular ventilation. Increased permeability, eventually leading to fatal edema, but ε-tox...

AI Technical Summary

Problems solved by technology

These vaccines have a certain effect on the prevention of the disease, but there are also some shortcomings: the most important thing is that the active ingredients in the vaccine are mainly bacteria antigens, and the antibodies induced by the body can only neutralize the bacteria antigens, but it is difficult to neutralize the bacteria antigens. and toxin antigens, which are not fully consistent with the pathogenic mechanism of "sudden death syndrome" in livestock

Therefore, there are still some doubts about the effectiveness of inactivated vaccines