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Trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and preparation method of trichoderma atroviride preparation thereof

A technology of Trichoderma dark green, Trichoderma dark green, applied in the direction of botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of drug resistance, environmental pollution, pesticide residues, etc., and achieve the goal of prevention and control Effects of increased range, increased control efficacy, pronounced inducement and growth-promoting effects

Active Publication Date: 2015-07-01
天津市农业科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to provide a trichoderma dark green preparation for preventing and controlling vegetable diseases and a preparation method thereof, which can be used for preventing and controlling fungal diseases of vegetables, and solve the problems of pesticide residues, drug resistance and environmental pollution caused by chemical pesticide control

Method used

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  • Trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and preparation method of trichoderma atroviride preparation thereof
  • Trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and preparation method of trichoderma atroviride preparation thereof
  • Trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and preparation method of trichoderma atroviride preparation thereof

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Experimental program
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Effect test

Embodiment 1

[0041] Screening, isolation and identification of Trichoderma dark green.

[0042] Antagonistic Trichoderma was screened by plate confrontation culture, and Trichoderma dark green Tr1208 was found to be effective against leek gray mold, tomato gray mold, cucumber brown spot, tomato early blight, melon and fruit fusarium wilt, melon and fruit root rot and other pathogens The fungus has a strong antagonistic effect, and it has a strong inhibitory effect on various vegetable pathogenic bacteria after testing.

[0043] Table 1. Antibacterial effect of Trichoderma dark green Tr1208 on cinerea cinerea, cucumber brown spot, tomato early blight, melon and fruit wilt, and melon and fruit root rot

[0044]

[0045] Trichoderma strain Tr1208 grows rapidly on the PDA medium, the growth diameter of the colony is greater than 90mm within 3 days, the surface of the mycelium is loose and flat, and the growth is radial; after 3 days of cultivation, it can be seen that the spores are obvious...

Embodiment 2

[0051] Fermentative preparation of Trichoderma dark green Tr1208.

[0052] 1) Strain activation: Trichoderma dark green Tr1208 ( Trichoderma atroviride ) strains were activated in potato sucrose agar medium, cultured at 25°C for 72-96 hours, and set aside;

[0053] 2) Seed liquid preparation: the activated Trichoderma dark green Tr1208 strain was cultured with shaking in wheat flour culture solution for 72-96 hours at 23-28°C, and the obtained Trichoderma dark green Tr1208 seed liquid after cultivation;

[0054] 3) Solid fermentation culture: The initial pH of the solid fermentation medium is between 6.5 and 7.5, and it is sterilized at high temperature. After the temperature drops below 30°C, insert the seed solution in step 2), and then at a temperature of 20~30°C, Under the condition of regular stirring (interval 24~30h), ferment and cultivate for 96~148h.

[0055] The composition of the solid fermentation medium formula one (w / w) is: 2% wheat flour, 12% wheat bran, 84% ...

Embodiment 3

[0062] The dark green Trichoderma inoculum is characterized in that it is composed of the following components: the dark green Trichoderma Tr1208 with the preservation number is 1.0×10 8 spores / gram (preparation method is the same as embodiment 2),

[0063] Xanthan gum 0.05%,

[0064] Sucrose 1%,

[0065] Sodium Humate or Potassium Humate 0.05%,

[0066] The remaining amount is calcium carbonate and diatomite carrier, wherein the ratio of calcium carbonate:diatomite in parts by weight=1:2.

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Abstract

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a trichoderma dark green preparation antagonizing vegetable pathogenic fungi, a preparation method and application thereof. Background technique [0002] With the acceleration of the adjustment of my country's agricultural structure and the improvement of people's living standards, the demand for diversification of vegetable varieties and annual balanced supply is increasing day by day. The second largest crop for food. According to statistics from the Ministry of Agriculture, in 2009 my country's vegetable planting area accounted for 43% of the world's total, and its total output accounted for 49.6% of the world's total. The total output and export volume both rank first in the world, and it can be called the world's largest vegetable country. [0003] However, with the development of vegetable production in China, the diversity of cultivation patter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 王勇高苇王万立张春祥郝永娟刘春艳霍建飞
Owner 天津市农业科学院
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