Culturing medium and culturing method for producing erythrocin through fermentation of streptomyces erythreus

The technology of a fermentation medium and a culture method is applied in the field of medium for producing erythromycin by fermentation of Streptomyces erythromycin, and can solve the problems of large inoculation amount, lowering of medium quality, darkening of the color of seed liquid and fermentation liquid, and the like, Achieve the effect of reducing procurement and production costs, reducing unfavorable factors, and improving mycelial morphology

Active Publication Date: 2014-01-01
HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 Increase the procurement cost of the enterprise
The composition of erythromycin seed medium and fermentation medium nitrogen source is at least 3 kinds, which increases the procurement workload and procurement cost of the enterprise
[0006] 3 The inoculum volume of primary seed culture is relatively large
The production scale of erythromycin in some domestic manufacturers can reach 10,000 tons per year, and the volume of the first-level seed culture fermentation tank exceeds 5m 3 , the inoculum volume of primary seed culture of conventional formula is 1.5~2L / M 3 , increasing the work intensity and workload of strain researchers
[0007] 4 The period of seed cultivation is long
[0008] 5 The fermentation unit of erythromycin is low
[0009] 6 During the sterilization process of the conventional medium, part of the glucose is easily carbonized at high temperature, which makes the color of the seed liquid and fermentation liquid darker, reduces the quality of the medium, and affects the effect of seed culture and fermentation culture

Method used

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  • Culturing medium and culturing method for producing erythrocin through fermentation of streptomyces erythreus
  • Culturing medium and culturing method for producing erythrocin through fermentation of streptomyces erythreus
  • Culturing medium and culturing method for producing erythrocin through fermentation of streptomyces erythreus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Quality of brewer's yeast dry residue:

[0088] Protein 57.5%; Phosphorus 87.4ug / ml; Moisture 4.2%; Ash 4.1%; Carbohydrate 12.8%;

[0089] Earthworm powder quality:

[0090] Protein 63.7%; moisture 8.4%, all passed through 80 mesh sieve.

[0091] Prepare 5m 3 First-class seed liquid, including 75kg of sugarcane molasses, 25L of soybean oil, 100kg of beer yeast dry residue, 40kg of earthworm powder, 0.15kg of ammonium sulfate, 4.5kg of sodium chloride, 1kg of light calcium carbonate, and 0.05kg of silicone oil defoamer.

[0092] Prepare 20m 3 Secondary seed liquid, including 500kg of sugarcane molasses, 240L of soybean oil, 500kg of beer yeast dry residue, 220kg of earthworm powder, 1.2kg of ammonium sulfate, 18kg of sodium chloride, 8kg of light calcium carbonate, and 0.4kg of silicone oil defoamer.

[0093] Preparation 180m 3 The composition of the fermentation medium is: 3960kg of sugarcane molasses, 1800L of soybean oil, 5400kg of beer yeast dry residue, 2700kg ...

Embodiment 2

[0100] Quality of brewer's yeast dry residue:

[0101] Protein 54.3%; phosphorus 85.1ug / ml; moisture 4.6%; ash content 3.8%; carbohydrate 13.5%;

[0102] Earthworm powder quality:

[0103] Protein 62.1%; moisture 8.2%, all passed through 80 mesh sieve.

[0104] Prepare 5m 3 First-class seed liquid, including 80kg of sugarcane molasses, 30L of corn oil, 105kg of beer yeast dry residue, 45kg of earthworm powder, 0.15kg of ammonium sulfate, 5kg of sodium chloride, 1.5kg of light calcium carbonate, and 0.1kg of silicone oil defoamer.

[0105] Prepare 20m 3 Secondary seed liquid, including 520kg of sugarcane molasses, 260L of corn oil, 520kg of beer yeast dry residue, 240kg of earthworm powder, 1.4kg of ammonium sulfate, 20kg of sodium chloride, 10kg of light calcium carbonate, and 0.6kg of silicone oil defoamer.

[0106] Preparation 180m 3The composition of the above fermentation medium is: 4860kg of sugarcane molasses, 1980L of corn oil, 5760kg of beer yeast dry residue, 288...

Embodiment 3

[0113] Quality of brewer's yeast dry residue:

[0114] Protein 54.9%; phosphorus 84.8ug / ml; moisture 4.5%; ash content 3.9%; carbohydrate 13.8%;

[0115] Earthworm powder quality:

[0116] Protein 62.4%; moisture 8.5%, all passed through 80 mesh sieve.

[0117] Prepare 5m 3 First-grade seed solution, including 85kg of sugarcane molasses, 35L of rapeseed oil, 115kg of beer yeast dry residue, 50kg of earthworm powder, 0.20kg of ammonium sulfate, 5kg of sodium chloride, 1.5kg of light calcium carbonate, and 0.1kg of silicone oil defoamer.

[0118] Prepare 20m 3 Secondary seed liquid, including 540kg of sugarcane molasses, 280L of rapeseed oil, 540kg of beer yeast dry residue, 260kg of earthworm powder, 1.44kg of ammonium sulfate, 19kg of sodium chloride, 10.4kg of light calcium carbonate, and 0.61kg of silicone oil defoamer.

[0119] Preparation 180m 3 The composition of the above fermentation medium is: 5040kg of sugarcane molasses, 2340L of rapeseed oil, 5940kg of beer yea...

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Abstract

The invention relates to a culturing medium and a culturing method for producing erythrocin through fermentation of streptomyces erythreus. A primary seed medium, a secondary seed medium and a fermentation medium all contains cane molasses, beer yeast dregs and earthworm powder. According to the invention, the cane molasses is used to substitute glucose; the beer yeast dregs and the earthworm powder are used to substitute soybean powder, peptone, peanut meal, and corn steep liquor; the culturing medium recipe is optimized, so that the cost problem of raw materials is solved, the raw materials are kept away from influence of sources to the maximum degree, the abundant supply is guaranteed, and the stable and efficiency production of erythrocin is realized; the culturing medium can increase fermentation unit, shorten fermentation period, and increase the content of erythrocin A.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a culture method for producing erythromycin by fermentation of Streptomyces erythromycin. Background technique [0002] Erythromycin is a macrolide antibiotic with an antibacterial spectrum similar to that of penicillin. [0003] At present, domestic manufacturers use red streptomycin to produce erythromycin in a three-stage fermentation mode. Its seed medium and fermentation medium mainly use starch and glucose as carbon sources; bean cake powder, peanut cake powder, corn steep liquor or peptone as nitrogen source, and use conventional raw and auxiliary materials to ferment and produce erythromycin. The main problems are: [0004] 1 High fermentation cost. With this formula, the consumption of raw and auxiliary materials accounts for about 65% of the production cost. [0005] 2 Increase the procurement cost of the enterprise. The erythro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/465
Inventor 任勇奇乃王忠中
Owner HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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