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Walnut protein peptide, preparation method and application thereof

A walnut protein and walnut technology, applied in the field of walnut protein peptide and its preparation, can solve problems such as waste of resources, and achieve the effects of high rate of return, small molecular weight and simple process

Active Publication Date: 2014-01-01
WUHAN JIUSHENGTANG BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The remaining walnut meal from walnut oil production is mostly used as raw material for animal feed, resulting in waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of walnut protein peptide

[0029] (1) Preparation of walnut protein isolate

[0030] 1. Obtaining the remaining walnut meal from walnut oil production: use a shelling tool to remove the walnut shell to obtain walnut kernels; then pour the walnut kernels into the drying pool, heat the walnut kernels to 40°C with a hot air stove and make them evenly heated , dried for 3 hours; finally put the pretreated walnut kernels into a mesh filter bag, then put the filter bag into a hydraulic oil press and start pressing to remove the oil, and the solid matter obtained is walnut meal.

[0031] 2. Extract protein from walnut dregs remaining from walnut oil production: Add walnut dregs after oil extraction to water, add 1g of walnut dregs to every 30ml of water, adjust pH to 8.5 with sodium hydroxide, and walnut dregs at 50°C Ultrasonic extraction was used for 60 minutes, then the pH value was adjusted with glacial acetic acid, acid precipitation was ca...

Embodiment 2

[0036] Embodiment 2, the molecular weight distribution detection of walnut protein peptide

[0037] The assay method of the molecular weight distribution of walnut protein peptide adopts high-efficiency gel filtration chromatography, test result shows that the relative molecular mass of walnut protein peptide provided by the invention mainly concentrates on the small molecule peptide and amino acid mixture of 0-1000Da, and small peptide content is 65 %above.

Embodiment 3

[0044] Embodiment 3, the antihypertensive pharmacodynamics test of walnut protein peptide

[0045] The antihypertensive pharmacodynamics of walnut protein peptide was verified by the detection experiment of angiotensin-converting enzyme (ACE) inhibitory activity.

[0046] (1) Preparation of reagents and sample solutions to be tested

[0047] Preparation of ACE solution: Dissolve 1U ACE in 2ml of 0.1mol / L boric acid buffer solution (pH8.3, containing 0.4mol / L NaCl).

[0048] Preparation of HHL solution: Take an appropriate amount of HHL and dissolve it in 0.1mol / L boric acid buffer (pH8.3, containing 0.4mol / L NaCl) to prepare a 5mmol / L HHL solution.

[0049] Preparation of hippuric acid standard solution: Take an appropriate amount of hippuric acid standard solution, and use 0.1mol / L boric acid buffer solution (pH8.3, containing 0.4mol / L NaCl) to prepare hippuric acid standard solutions with different concentrations.

[0050] Preparation of walnut protein peptide samples: tak...

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Abstract

The present invention discloses a walnut protein peptide, a preparation method and an application thereof. The walnut protein peptide is prepared according to the following steps: adopting aromatic fruit plant composite protease to carry out an enzymolysis reaction on walnut separation protein to obtain the walnut protein peptide, wherein the relative molecular mass of the walnut protein peptide is mainly between 0-1000 Da, and the walnut protein peptide comprises 2-9 amino acids, has characteristics of unique flavor, high peptide content, small molecular weight, short peptide chain, no requirement of debittering, strong activity and strong diversity, and can be used as a pharmaceutical raw material and a food additive for preparation of products with effects of blood pressure lowering, anti-oxidation, memory enhancement, and brain nerve sensitivity improvement, wherein the products comprise drugs, health care products, food and the like. The preparation method for preparing the walnut protein peptide has characteristics of simple process, less investment, quick effect, high return rate, no environmental pollution, low carbon and high efficiency, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a walnut protein peptide, a preparation method thereof and an application thereof. Background technique [0002] Walnut is a Juglandaceae Juglans plant and is one of the four largest dry fruits in the world. The total amount of saturated fatty acids in walnut oil is generally less than 10%, and the content of unsaturated fatty acids is rich, mainly including oleic acid, linoleic acid and linolenic acid, the total amount is about 90%. [0003] The content of protein in walnut kernel is about 15%, which contains 8 kinds of essential amino acids for human body, and the content is reasonable, close to the standards stipulated by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). It is a good woody plant protein. The remaining walnut meal from walnut oil production is mostly used as raw material for animal feed, resulti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00A61K38/01A61P9/12A61P39/06A61P25/00A23L1/29A23L33/00
Inventor 邹远东
Owner WUHAN JIUSHENGTANG BIOENG
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