Ultra wide band stimulated raman spectroscopy microscopic imaging system simple and convenient to use

Abstract

The invention discloses an ultra wide band stimulated raman spectroscopy microscopic imaging system simple and convenient to use. The ultra wide band stimulated raman spectroscopy microscopic imaging system is obtained through the method including the steps that ultra-short pulse lasers generated by an ultra-short pulse laser are divided into two beams through a spectroscope, one of the beams is strokes light which sequentially passes through an adjustable retroreflection mirror system with a rotary knob, and a light intensity modulator, the other beam generates a super-continuum spectrum through a photonic crystal fiber, and a part of the wave band of the super-continuum spectrum is selected through a band-pass filter to be used as pump light, time relay of two pulses is rapidly adjusted through the retroreflection mirror system after the two beams are converged through a dichroscope, the components, with different wavelengths, of the strokes light and the components, with different wavelengths, of the pump light are overlapped, sequentially pass through a scanning unit, an objective lens, an optical filter and a photoelectric detector, and are processed through a lock-in amplifier and imaged on a computer, the continuous adjustable ultra wide band stimulated raman biological microscope spectral imaging is finally achieved, and the ultra wide band spectral component continuous adjustable stimulated raman spectrum detection imaging which has great research value in the bioscience field can be specifically achieved.

Description

technical field [0001] The invention relates to the fields of optical microscopic technology, optical fiber technology and biological detection technology, in particular to a simple ultra-broadband stimulated Raman spectrum microscopic imaging system. Background technique [0002] In optical bioimaging systems, probe-labeled fluorescence microscopy, especially confocal fluorescence microscopy based on fluorescent single-molecule processes, has been widely used due to its advantages of high resolution, high sensitivity, and high magnification. However, since some biomolecules have no obvious photoluminescent properties or labeled probes are easy to cause biological damage, new label-free, high-specificity, high-contrast bioimaging methods, such as stimulated Raman scattering imaging, Coherent anti-Stokes Raman scattering imaging, stimulated fluorescence imaging, etc. [0003] Among them, the stimulated Raman scattering microscopic imaging is irradiated by two beams with a sp...

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Problems solved by technology

[0004] In a large number of studies in the past, the stimulated Raman microscopy system used two titanium sapphire lasers or a titanium sapphire laser and an optical parametric oscillator as the light sources of the two beams respectively. This dual light source system is expensive. Huge volume, complex system, cumbersome pulse tuning and synchronization, and it is difficult to achieve continuously adjustable ultra-broadband spectral imaging of samples in different Raman spectral ranges, even if some researchers can use some methods to achieve certain Raman spectral imaging. Continuous imaging of the spectral range, but its imaging spectral range is also very limited

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