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Preparation method of dual functional chitosan microspheres

A technology of chitosan microspheres and dual functions, which is applied in the field of preparation of chitosan microspheres, can solve problems such as the difficulty in preparing monodisperse cross-linked particles, and achieve environmental protection and economy of experimental materials, convenient operation process, and adjustable size. control effect

Active Publication Date: 2014-01-08
黄山市开发投资集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in atomization drying or alkali precipitation technology, it is difficult to prepare cross-linked particles with good monodispersity [G.H.Ma., et al., Macromolecular Symposia, 2010, 288, 41–48.]

Method used

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  • Preparation method of dual functional chitosan microspheres
  • Preparation method of dual functional chitosan microspheres
  • Preparation method of dual functional chitosan microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Superparamagnetic Fe 3 O 4 Preparation of nanoparticles

[0039] Weigh 2.705g (0.01mol) FeCl 3 ·6H 2 O and 0.995g (0.005mol) FeCl 2 ·4H 2 O was dissolved in 100ml of deionized water, transferred to a 250ml three-neck flask, vigorously stirred under nitrogen protection and gradually heated to 80°C, after 5 minutes of constant temperature, 5ml of concentrated ammonia water (25wt%) was slowly added dropwise with a constant pressure burette, After 2 minutes of titration, measure the pH value of the solution, adjust the pH value to 8-9, and continue the constant temperature reaction for 20 minutes under the protection of nitrogen. After the reaction, the product was magnetically separated, washed with water for 3 times until the solution was neutral, and washed with ethanol for 2 times to obtain superparamagnetic Fe 3 O 4 Nanoparticles 1g. The prepared superparamagnetic Fe 3 O 4 Nanoparticles were dispersed in 100 mL ethanol solution to obtain superparamagnetic ...

Embodiment 2

[0045] 1. Superparamagnetic Fe 3 O 4 Preparation of nanoparticles

[0046] Weigh 2.705g (0.01mol) FeCl 3 ·6H 2 O and 0.995g (0.005mol) FeCl 2 ·4H 2 O was dissolved in 100ml of deionized water, transferred to a 250ml three-neck flask, vigorously stirred under nitrogen protection and gradually heated to 80°C, after 5 minutes of constant temperature, 5ml of concentrated ammonia water (25wt%) was slowly added dropwise with a constant pressure burette, After 2 minutes of titration, measure the pH value of the solution, adjust the pH value to 8-9, and continue the constant temperature reaction for 20 minutes under the protection of nitrogen. After the reaction, the product was magnetically separated, washed with water for 3 times until the solution was neutral, and washed with ethanol for 2 times to obtain superparamagnetic Fe 3 O 4 Nanoparticles 1g. The prepared superparamagnetic Fe 3 O 4 Nanoparticles were dispersed in 100 mL ethanol solution to obtain superparamagnetic ...

Embodiment 3

[0052] The preparation process of this example is the same as that of Example 1, the difference is that the needle type used in step 2) is 8# and the voltage is 30kv. The particle size of the bifunctional chitosan microspheres prepared in this example is about 1.5 μm.

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Abstract

The invention discloses a preparation method of dual functional chitosan microspheres. The preparation method comprises the following steps of 1, mixing an ethanol dispersion solution of superparamagnetic Fe3O4 nanoparticles and a deionized water solution of chitosan, and uniformly dispersing the mixture to obtain a mixed solution, 2, through a soybean oil solution of span-80, receiving the mixed solution obtained by electronic injection to obtain a magnetic chitosan microsphere dispersion liquid, and 3, dropwisely adding a cross-linking agent solution into the magnetic chitosan microsphere dispersion liquid and carrying out a reaction process after dropwise addition at a room temperature for 1.5-2.5h to obtain the dual functional chitosan microspheres. The dual functional chitosan microspheres have uniform and controllable sizes and structured morphology. The preparation method utilizes an autofluorescence substance to replace extraneous introduced fluorescence substances such as organic dye and quantum dots thereby avoiding the toxicity produced by the chitosan microspheres on cells in use and inhibiting cell secondary damage.

Description

1. Technical field [0001] The invention relates to a preparation method of chitosan microspheres, in particular to a preparation method of bifunctional chitosan microspheres. The bifunctional chitosan microspheres of the invention have both superparamagnetic and autofluorescence properties. 2. Background technology [0002] Magnetic chitosan particles are a typical multifunctional material, which combines the biocompatibility and magnetism of chitosan well, so it has been widely used in the field of biomedical research. For example, targeted therapy [Jana Chomoucka., et al., Pharmacological Research, 2010, 62(2), 144-149.], detection of lesion location [H.P.Shao., et al., IEEE Transactions on Magnetics, 2005, 41(10), 4102-4104.], and chemotherapy and thermal therapy [Ji-Ho Park., et al., Journal of Magnetism and Magnetic Materials, 2005, 293, 328–333.], etc. It is worth mentioning that researchers often tend to introduce fluorescent properties into magnetic chitosan, so as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/12C08L5/08C08K3/22B01J13/04A61K49/00
Inventor 杨文宋健刘威郝文涛
Owner 黄山市开发投资集团有限公司
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