Enzyme preparation for removing o-nitrobenzoic acid (2-NBA) and application
A technology of o-nitrobenzoic acid and enzyme preparation, which is applied in the fields of enzyme genetic engineering and enzyme engineering, can solve problems such as the reduction of decomposition rate of organic matter, and achieve the effects of excellent effect, mild reaction conditions and high enzyme activity
Inactive Publication Date: 2014-01-08
ZHEJIANG FORESTRY UNIVERSITY
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Abstract
The invention belongs to the field of enzyme gene engineering and enzyme engineering and relates to an enzyme preparation for removing o-nitrobenzoic acid (2-NBA) and an application. The enzyme preparation is an aqueous solution or a buffer solution and comprises a 2-NBA degrading enzyme and MgCl2, wherein the amino acid sequence of the 2-NBA degrading enzyme is shown in SEQ ID No.1; the final concentration of MgCl2 is 0.1-0.3mM; the pH value of a reaction system is 6.0-8.0; when the enzyme preparation is the buffer solution, the buffer solution is generally a sodium phosphate buffer solution or a potassium phosphate buffer solution with pH value of 7.2-7.7 and concentration of 50-100mM.
Application Domain
Contaminated soil reclamationOxidoreductases
Technology Topic
Nitrobenzoic acidChemistry +8
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