Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof

A compound bacterial agent and vegetable technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of cumbersome use methods, and achieve the effect of long peak maintenance time, beneficial to plant absorption, and improvement of soil quality.

Inactive Publication Date: 2014-01-22
辽宁省微生物科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Adding straw decomposing agent in the process of returning to the field is an effective way to solve this problem. Adding an activator at the same time as applying the straw decomposing agent can also speed up the straw decomposing speed, but this met

Method used

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  • Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof

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Embodiment Construction

[0022] 1. Composition and preparation of compound bacterial agent:

[0023] 1. Composition and preparation of liquid bacteria:

[0024] Trichoderma koningeni (Trichoderma koningii), Mucor racemosa (Mucor racemosus), Bacillus subtilis (Bacillus cereus. Frankland) The test tube slant culture medium is respectively inoculated in the corresponding liquid culture medium, and liquid strains are respectively made into liquid strains through liquid fermentation;

[0025] The weight composition of Trichoderma korningen culture medium: corn flour 2.0g, yeast extract 1.0g, potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.05g, calcium chloride 0.01g, water 100ml, pH natural.

[0026] The weight composition of Bacillus subtilis medium: glucose 2.0g, peptone 1.5g, sodium chloride 0.5g, beef extract 0.05g, agar 2.0g, water 100ml, pH natural.

[0027] The weight composition of Mucor racemosus culture medium: 3.0g peptone, 0.4g soluble starch, 0.2g magnesium sulfate, 0.2g potas...

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Abstract

The invention discloses a complex microbial inoculant for greenhouse vegetable production and preparation and application of the complex microbial inoculant. The complex microbial inoculant is composed of mixed cultures and additives, and the mixed cultures are composed of trichoderma koningii, mucor racemosus and Bacillus subtillis. A purified and rejuvenated trichoderma koningii test tube slant culture medium, a purified and rejuvenated mucor racemosus test tube slant culture medium and a purified and rejuvenated Bacillus subtillis test tube slant culture medium are inoculated to corresponding liquid culture media respectively, and liquid bacterium seeds are manufactured through liquid fermentation; the three kinds of fermented bacterium liquid are inoculated to solid culture materials, are cultured for 48-72 hours at 28-30 DEG C and are manufactured into the mixed cultures after being dried and smashed; the additives are formed by glucose, urea and humic acid, and the mixed cultures and the additives are mixed and manufactured into the complex microbial inoculant with the weight ratio of 2.0-5.0:1.0. The mixed cultures and the additives are mixed in use, the good cooperation function is achieved, the growth and reproduction capacity and the enzyme production capacity of flora are promoted, and straw degradation is accelerated. The quality of greenhouse soil is improved, the harm of nematodes is reduced, and compared with the average product with an applied single microbial inoculant, the average product with the applied complex microbial inoculant is improved by 5-8%.

Description

technical field [0001] The invention relates to a composite bacterial agent in the technical field of microorganisms, in particular to a composite bacterial agent for improving the soil quality of facility vegetables and used for facility vegetable production, as well as a preparation method thereof and an application method of the bacterial agent. Background technique [0002] my country is a large agricultural country, with an annual output of about 800 million tons of crop straw. Because it contains a lot of cellulose, its nutritional value is low, and it is difficult to be used by livestock and poultry. Therefore, only a small amount is used as feed, and most of it is discarded or burned, causing environmental pollution and waste of resources. Straw returning to the field is an important measure to implement the fertile soil project in my country, continuously improve the quality of cultivated land, and realize sustainable agricultural development. Adding straw-degrading...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C09K17/00C12R1/885C12R1/785C12R1/125C09K101/00C05G3/60
Inventor 李莉池景良吴红艳王志学冯健于淼方新李鑫宗玉丽
Owner 辽宁省微生物科学研究院
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