Method of producing low-temperature lipase by fermentation

A lipase, low temperature technology, applied in the field of low temperature lipase production by fermentation, can solve the problems of short time and harsh growth conditions restricting the development, development and research of low temperature lipase

Inactive Publication Date: 2014-02-12
HUNAN HONGYING BIOTECHNOLOGY CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lipase can be divided into acidic, neutral, and alkaline lipases according to their action pH; and can be divided into low-temperature lipase (cold-active lipase), medium-temperature lipase and high-temperature lipase according to their action temperature , Low-temperature lipase is mostly produced by low-temperature microorganisms. The growth conditions of such bacteria are relatively harsh, which seriously restricts the development of low-temperature lipase, so the development and research time is relatively short. However, low-temperature lipase has high catalytic activity in low-temperature environments. Its activation energy and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Pick a single colony from the slant, streak it on the LB plate medium (peptone 10g / L, sodium chloride 10g / L, yeast extract juice 5g / L, agar 20g / L, pH 7.2), place the plate on Incubate in a 30°C incubator for 24 hours.

[0023] 2. Inoculate 1 loop of bacteria from the plate into a 250ml Erlenmeyer flask containing 50ml of seed medium (peptone 10g / L, sodium chloride 10g / L, yeast extract 5g / L, pH 7.0), and inoculate 10 bottles were cultured on a shaker, culture conditions: temperature 30°C, rotation speed 200r / min, cultured for 8 hours to obtain fermented seed liquid.

[0024] 3. Add 450ml of the fermented seed solution to 4.5 liters of enzyme-producing medium (beef extract 40g / L, olive oil 10ml / L, Triton X-1005ml / L, initial pH=7.0, bubble enemy 3ml / L) in a 7-liter fermenter, set the fermentation parameters, temperature 30°C, pH 7.0, speed 350r / min, ventilation 1.5L / min / L, take out after 35 hours of fermentation;

[0025] 4. Preparation of lipase solution

[0026] Ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method of producing low-temperature lipase by fermentation. The low-temperature lipase is prepared by using a self-screened wild bacterial strain as starting culture and adopting an optimized seed culture medium, an enzyme-production culture medium as well as a fermentation production technical method. Fermentation liquor has highest enzyme activity of over 6000 U/L; the produced low-temperature lipase has an optimal temperature of 30 DEG C, still has 65% of relative enzyme activity at 0 DEG C, and can be widely applied to the fields such as detergents, food industry, papermaking, medicines, leather and environmental protection, which belong to the biological technical field.

Description

technical field [0001] A method for fermenting and producing low-temperature lipase provided by the invention is to prepare low-temperature lipase by using the wild bacterial strain screened by oneself as the starting strain, and adopting optimized seed culture medium, enzyme-producing medium and fermentation production technology method to prepare low-temperature lipase. The highest enzyme activity of the prepared low-temperature lipase can reach more than 6000U / L, the optimal temperature of the produced low-temperature lipase is 30°C, and it still has 65% relative enzyme activity at 0°C, which can be widely used in detergent, food industry, papermaking , medicine, leather, environmental restoration and other fields, which belong to the field of biotechnology. Background technique [0002] Lipase (lipase, EC3.1.1.3), also known as triacylglycerol acylhyrolase, can catalyze the hydrolysis of its water-insoluble natural substrate triglyceride into fatty acids and glycerol at ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/20
CPCC12N9/20C12Y301/01003
Inventor 李洪兵张峰张明
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap