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Application of acorus gramineus polysaccharide in preparing healthcare food having neuroprotective effect

A neuroprotective and health food technology, applied in the function, application, food preparation and other directions of food ingredients, can solve problems such as lack of research on nervous system diseases, and achieve the effects of preventing and treating nervous system diseases, less reagent consumption, and simple process

Active Publication Date: 2014-03-05
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research and development of the active function of Acorus polysaccharides is still relatively small, and there is a lack of related research on neurological diseases.

Method used

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  • Application of acorus gramineus polysaccharide in preparing healthcare food having neuroprotective effect
  • Application of acorus gramineus polysaccharide in preparing healthcare food having neuroprotective effect
  • Application of acorus gramineus polysaccharide in preparing healthcare food having neuroprotective effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This example provides a calamus polysaccharide, which is prepared by the following method: after crushing the rhizomes of calamus, adding ethanol according to the ratio of solid to liquid 1:10 (g / mL), heating and stirring at 70°C for 3 hours, and filtering under reduced pressure Solvents, recovery of medicinal materials. Add water according to the solid-liquid ratio of 1:10 (g / mL), heat and stir at 95°C for 3 hours, and collect the water extract. Concentrate under reduced pressure at 60°C, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clear concentrated extract. Add 4 times the volume of ethanol to the above extract, mix well, let stand overnight at 4° C. to precipitate, collect the precipitate by centrifugation and vacuum freeze-dry. Dissolve the precipitate in water and pass through a DEAE-sepharose anion-exchange dextran gel column, then use water and 1.0mol / L NaCl solution to elute sequentially, collect the eluate of 1.0mol...

Embodiment 2

[0026] This example provides an acidic polysaccharide of Acorus calamus, which is prepared by the following method: after crushing the rhizome of Calamus calamus, adding ethanol according to the ratio of solid to liquid of 1:20 (g / mL), heating and stirring at 80°C for 3 hours, and filtering under reduced pressure The solvent is removed and the medicinal materials are recovered. Add water according to the solid-liquid ratio of 1:10 (g / mL), heat and stir at 100°C for 3 hours, and collect the water extract. Concentrate under reduced pressure at 55°C, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clear concentrated extract. Add 4 times the volume of ethanol to the above extract, mix well, let stand overnight at 4° C. to precipitate, collect the precipitate by centrifugation and vacuum freeze-dry. Dissolve the precipitate in water and pass through a DEAE-sephadex A-25 anion-exchange cellulose column, elute with water and 2.0mol / L KCl solut...

Embodiment 3

[0028] This example provides an acidic polysaccharide of Acorus calamus, which is prepared by the following method: after crushing the rhizome of Calamus calamus, adding ethanol according to the ratio of solid to liquid 1:5 (g / mL), heating and stirring at 85°C for reflux for 3 hours, and filtering under reduced pressure The solvent is removed and the medicinal materials are recovered. Add water according to the solid-liquid ratio of 1:15 (g / mL), heat and stir at 90°C for 4 hours, and collect the water extract. Concentrate under reduced pressure at 50°C, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clear concentrated extract. 5 times the volume of ethanol was added to the above extract, mixed evenly and left to settle at 4°C overnight, and the precipitate was collected by centrifugation and vacuum freeze-dried. Dissolve the precipitate in water and pass it through D900 type anion exchange resin, then use water and 1.0mol / L ammonium ch...

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Abstract

The invention discloses application of acorus gramineus polysaccharide in preparing healthcare food having a neuroprotective effect, and simultaneously discloses a preparation method of the acorus gramineus polysaccharide. The method comprises the steps of implementing degreasing, water extraction and alcohol precipitation, ion exchange and dialysis to acorus gramineus to obtain the acorus gramineus polysaccharide. The preparation method is simple, stable and efficient, and is suitable for industrial production. Mammalian cell and caenorhabditis elegans disease model tests prove that the acorus gramineus polysaccharide disclosed by the invention can obviously relieve oxidative stress toxicity of hydrogen peroxide on nerve cells, enhance autioxidant enzyme activity, reduce lipid peroxidation product content and inhibit apoptosis, and can inhibit neurotoxicity of beta-amyloid protein, which shows an excellent neuroprotective effect.

Description

technical field [0001] The invention belongs to the technical field of health food / health food, and in particular relates to the application of calamus polysaccharide in preparing health food with neuroprotective effect. technical background [0002] With the intensification of the aging trend of the global population, neurological diseases characterized by the gradual decline and even death of specific nerve cells and tissues have become major diseases that seriously endanger human life, health and quality of life. Deep focus. Nervous system diseases have complex pathological processes, and a large number of studies have shown that they are affected by factors such as oxidative stress and abnormal protein aggregation. For example, when the nervous system is subjected to oxidative stress, the level of endogenous active oxygen free radicals increases, which will promote the imbalance of redox metabolism in cells, resulting in the decrease of antioxidant enzyme activity and d...

Claims

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Application Information

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IPC IPC(8): A23L1/09C08B37/00A23L33/125
CPCA23L29/30A23L33/00A23V2002/00C08B37/0003A23V2200/30A23V2200/302A23V2300/14A23V2300/30
Inventor 马方励李海峰胡明华梁明马忠华汪强强张婉婉
Owner INFINITUS (CHINA) CO LTD
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