Infectious pancreatic necrosis virus standard sample and preparation method thereof
A standard sample, infectious technology for biochemical equipment and methods, viruses/phages, microbe-based methods, etc., which can solve problems such as time-consuming
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Embodiment 1 standard sample preparation
[0030] 1. Treatment of tissue disease material: Homogenize the obtained tissue disease material samples (including brain, liver, spleen and kidney) with a tissue grinder. / mL streptomycin) in M199 cell culture medium, suspended at a final dilution of 1:10, and incubated overnight at 4°C. Centrifuge at 7000rpm / min for 15min, and collect the supernatant. Dilute the 1:10 diluted tissue homogenate supernatant with cell culture medium for two 10-fold dilutions, and then use sterile The operation method is to inoculate CHSE-214 monolayer cells with an appropriate volume into CHSE-214 monolayer cells that have grown for about 24 hours, and after adsorption at 20°C for 1 hour, add cell culture medium and culture in a low-temperature incubator at 20°C. After the cells were infected with the virus and fell off, the cell culture was repeatedly frozen and thawed three times after cytopathic pathology (CPE) appeared, and centrifuged at 10...
Embodiment 2
[0050] Embodiment 2 uniformity test
[0051] Regardless of whether the initial homogeneity inspection has been carried out during the preparation process, all standard samples prepared in batches and packaged into the smallest packaging unit must be inspected for uniformity. For standard samples that are graded and packaged, uniformity testing is required when the bulk package is packaged into the smallest packaging unit.
[0052] The fixed value of the freeze-dried sample: According to the IPNV ELISA method in the quarantine method of GB15805.1-2008 infectious pancreatic necrosis virus, according to the IPNV ELISA detection kit, the qualitative test of the standard sample of infectious pancreatic necrosis virus was carried out, See below for the test procedure.
[0053] Immediately after lyophilization, 1×8 samples A and 1×8 samples B were randomly selected for homogeneity testing.
[0054] ANOVA formula
[0055] Table 1 ANOVA formula
[0056]
[0057] C ...
Embodiment 3
[0069] Embodiment 3 stability test
[0070] For a period of two years, take two samples of the prepared standard sample in stages each time, and carry out its content determination according to different storage conditions. The results were calculated according to the above-mentioned determination methods. Standard samples should be regularly tested for stability of undetermined characteristic values under specified storage or use conditions.
[0071] Standard samples were tested for stability under two temperature types (IPNV ELISA method in GB15805.1-2008 Infectious Pancreatic Necrosis Virus Quarantine Methods): one is at storage temperature (-20°C) Stability test, 24 samples (sample A and B) were randomly taken, and 2 samples were tested every month for a total of 12 months; the other was the stability at a higher temperature (simulating the transport condition of the sample at 4°C) For the test, 24 samples were taken, and 2 samples were tested every day for a total of ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More