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Compound photosynthetic bacteria agent and preparation method thereof

A technology of photosynthetic bacteria agent and bacteria quantity, which is applied in the field of composite photosynthetic bacteria agent and its preparation, can solve the problems of complex mixing, unfavorable microbial agent cost control and large-scale production, etc., so as to prolong the harvest period, increase yield and The effect of unit weight and thin peel

Inactive Publication Date: 2014-03-19
SHIJIAZHUANG RENHONG AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the patent CN200910219977.1 open class is a compound bacterial agent containing various microorganisms, which can increase the yield and improve the quality, but the compound bacterial agent needs to be used in conjunction with farmyard manure, humic acid, oxalic acid and other substances, and the mixing is more complicated.
[0004] The preparation of microbial fertilizer often needs to go through strict procedures such as strain selection and classification expansion, and it is necessary to sterilize the medium and ensure that the operations of each step are carried out in a sterile environment, which is harmful to the operating environment and technical personnel. Higher requirements are not conducive to cost control and large-scale production of microbial agents

Method used

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  • Compound photosynthetic bacteria agent and preparation method thereof
  • Compound photosynthetic bacteria agent and preparation method thereof
  • Compound photosynthetic bacteria agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The formula of the medium is Na 2 HPO 4 0.3g / ml, MgCl 2 0.12g / ml, NaCl 1.2g / ml, yeast extract 1.2g / ml, NaHCO 3 30g / ml, sodium acetate 1.2g / ml, ammonium sulfate 0.6g / ml, the rest water, pH 8.5.

[0028] production method:

[0029] Use raw water to prepare pure water through a pure water machine, and adjust the pH to 8.5. Then configure the liquid medium according to the process formula, put in the strains, and the inoculation amount is 3%, including 0.9% of Rhodopseudomonas sphaericus, 1.8% of Rhodopseudomonas palustris, and 0.3% of Rhodospirillum rubrum.

[0030] Pack the prepared medium into 10L narrow-mouth bottles, and the feeding volume accounts for 85% of the volume of the narrow-mouth bottles. A group of 10 bottles is placed on a culture rack, cultivated with a 100w incandescent lamp for 5 days, and the finished product is tested. The miscellaneous bacteria rate is less than 10%, and the finished product is not easy to be contaminated with bacteria and can...

Embodiment 2

[0033] The formula of the medium is Na 2 HPO 4 0.3g / ml, MgCl 2 0.12g / ml, NaCl 1.2g / ml, yeast extract 1.2g / ml, NaHCO 3 30g / ml, sodium acetate 1.2g / ml, ammonium sulfate 0.6g / ml, the rest water, pH 8.5.

[0034] production method:

[0035] Use raw water to prepare pure water through a pure water machine, and adjust the pH to 8.5. Then configure the liquid medium according to the process formula, put in the strains, and the inoculation amount is 2%, including 0.6% of Rhodopseudomonas sphaericus, 1.2% of Rhodopseudomonas palustris, and 0.2% of Rhodospirillum rubrum.

[0036] Pack the prepared medium into 10L narrow-mouth bottles, and the feeding volume accounts for 70% of the volume of the narrow-mouth bottles. A group of 10 bottles is placed on a culture rack, cultivated with a 100w incandescent lamp for 5 days, and the finished product is tested. The miscellaneous bacteria rate is less than 10%, and the finished product is not easy to be contaminated with bacteria and can...

Embodiment 3

[0039] The formula of the medium is Na 2 HPO 4 0.3g / ml, MgCl 2 0.12g / ml, NaCl 1.2g / ml, yeast extract 1.2g / ml, NaHCO 330g / ml, sodium acetate 1.2g / ml, ammonium sulfate 0.6g / ml, the rest water, pH 8.5.

[0040] production method:

[0041] Use raw water to prepare pure water through a pure water machine, and adjust the pH to 8.5. Then configure the liquid medium according to the process formula, put in the strains, and the inoculation amount is 4%, including 0.6% of Rhodopseudomonas sphaericus, 1.2% of Rhodopseudomonas palustris, and 0.2% of Rhodospirillum rubrum.

[0042] Pack the prepared medium into 10L narrow-mouth bottles, and the feeding volume accounts for 80% of the volume of the narrow-mouth bottles. A group of 10 bottles is placed on a culture rack, cultivated with a 100w incandescent lamp for 5 days, and the finished product is tested. The miscellaneous bacteria rate is less than 10%, and the finished product is not easy to be contaminated with bacteria and can b...

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PUM

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Abstract

The invention relates to an agricultural bacterial manure, and particularly relates to a compound photosynthetic bacteria agent and a preparation method thereof. The total effective viable count of the compound photosynthetic bacteria agent is not less than 2*10<8> / ml, wherein the compound photosynthetic bacteria agent contains 25-35 percent of spherical rhodopseudomonas spheroids, 55-65 percent of rhodopseudomonas palustris and 5-15 percent of rhodopseudomonas rubrum. The compound photosynthetic bacteria agent has functions of increasing the yield of vegetables and fruits and improving the quality of the fruits and the vegetables, is capable of reducing the content of heavy metals, is safe in use, and has no problem of pesticide residue. The compound photosynthetic bacteria agent provided by the invention can be prepared by adopting a simple method; a culture medium is not required to be sterilized and subjected to propagation for a plurality of times, and thus the production flow is greatly simplified and the production cost is lowered.

Description

technical field [0001] The invention relates to an agricultural microbial bacterial fertilizer, in particular to a composite photosynthetic bacterial agent and a preparation method thereof. Background technique [0002] Microbial fertilizer is a kind of product that makes crops obtain specific fertilizer effects by the life activities of microorganisms. Crops can obtain specific fertilizer effects to promote crop growth, increase yield, and improve quality. In this effect, the active microorganisms in the product play a key role, and general microbial fertilizers are non-toxic, harmless, without any pollution and can promote the growth of crops. Photosynthetic bacteria are a kind of beneficial microbial preparations mainly composed of photosynthetic bacteria cultivated under specific conditions. Photosynthetic bacteria are rich in nutrients and a variety of active substances, which determines that photosynthetic bacteria have many known and unknown functions and effects. S...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01C12R1/38
Inventor 王柏群
Owner SHIJIAZHUANG RENHONG AGRI TECH CO LTD
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