Method for detecting Hg<2+> by fluorescence quenching

A fluorescence quenching and detection method technology, applied in the field of physical chemistry, can solve the problems of high cost and complicated operation, and achieve the effects of low cost, high sensitivity and selectivity, and short time consumption

Inactive Publication Date: 2014-03-19
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods mainly used in the detection of heavy metal ions in water mainly include complexometric titration, spectrophotometry, chemiluminescence, electrochemical analysis, high performance liquid chromatography, ion chromatography, atomic spectroscopy and mass spectrometry, etc. , however, most of these methods are complicated to operate, expensive, and require special detection instruments

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  • Method for detecting Hg&lt;2+&gt; by fluorescence quenching
  • Method for detecting Hg&lt;2+&gt; by fluorescence quenching
  • Method for detecting Hg&lt;2+&gt; by fluorescence quenching

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Detection of Yellow River Raw Water Samples in Qilihe District, Lanzhou Section

[0027] After the Yellow River water samples were collected, they were filtered three times with absorbent cotton to remove a large amount of sediment, and stored in 4 0 C refrigerator is used as a test water sample for backup, prepare a number of semiconductor quantum dots containing about 1.8mg / mL, add 1~3mL PBS buffer solution (pH=7.04,) to it and shake well, measure the emission spectrum at a certain excitation wavelength, and then gradually Accumulatively add 8~12uL of the original water sample of the Yellow River, and the change results of the fluorescence intensity at the same excitation wavelength after adding different volumes of the Yellow River water sample to a certain molar amount of semiconductor quantum dots are shown in Figure 5 . Figure 5 In the same concentration of CdSe-CdS-NAC solution, after adding different volumes of Yellow River water samples, the change diagram ...

Embodiment 2

[0029] Detection of Raw Water Samples of the Yellow River in Chengguan District, Lanzhou Section

[0030] After the Yellow River water samples were collected, they were filtered three times with absorbent cotton to remove a large amount of sediment, and stored in 4 0 C refrigerator is used as a test water sample for backup, prepare a number of semiconductor quantum dots containing about 1.8mg / mL, add 1~3mL PBS buffer solution (pH=7.04,) to it and shake well, measure the emission spectrum at a certain excitation wavelength, and then gradually Accumulatively add 8~12uL of raw water samples from the Yellow River, and measure the change of fluorescence intensity at the same excitation wavelength after adding a certain molar amount of semiconductor quantum dots to different volumes of Yellow River water samples. By calculating the Hg in the water samples of the Yellow River 2+ The content is about 3.10~9.85ppm.

Embodiment 3

[0032] Detection of raw water samples of the Yellow River in Duanyantan, Lanzhou

[0033] After the Yellow River water samples were collected, they were filtered three times with absorbent cotton to remove a large amount of sediment, and stored in 4 0 C refrigerator is used as a test water sample for backup, prepare a number of semiconductor quantum dots containing about 1.8mg / mL, add 1~3mL PBS buffer solution (pH=7.04,) to it and shake well, measure the emission spectrum at a certain excitation wavelength, and then gradually Cumulatively add 8~12uL of raw water samples from the Yellow River, and measure the change in fluorescence intensity at the same excitation wavelength after adding a certain molar amount of semiconductor quantum dots to different volumes of Yellow River water samples. By calculating the Hg in the water samples of the Yellow River 2+ The content is about 3.12~9.88ppm.

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Abstract

The invention discloses a method for detecting Hg<2+> by fluorescence quenching. High-sensitivity and high-selectivity detection for the Hg<2+> in environment sewage is realized by the fluorescence quenching of a heavy metal ion Hg<2+> induction nuclear shell type CdSe-CdS-NAC semiconductor quantum dot. According to the method, the CdSe-CdS-NAC semiconductor quantum dot with stable aminothiopropionic acid is synthesized under the protection of inert gas in a water phase, and then the sensitivity of the CdSe-CdS-NAC semiconductor quantum dot to the Hg<2+> and the selective fluorescence quenching of the CdSe-CdS-NAC semiconductor quantum dot to different metal cations are detected in a lab; the experiment shows that the CdSe-CdS-NAC semiconductor quantum dot is high in sensitivity and selectivity; finally, detection for a Yellow River water sample and running water shows that the detection limit to Yellow River water is 3.05-9.88ppm; existence of the Hg<2+> in the running water and household water cannot be detected.

Description

technical field [0001] The invention relates to a detection method in the technical field of physical chemistry, in particular to a method for detecting Hg in various water sources 2+ content method. Background technique [0002] With the enhancement of people's awareness of environmental protection, more and more attention is paid to the pollution of heavy metal ions in the environment and domestic sewage, especially Pb 2+ , Hg 2+ 、Cd 2+ 、Cu 2+ As well as the pollution of water bodies by heavy metal elements with significant biological toxicity such as metal arsenic. Heavy metal ions are discharged directly into rivers, lakes or oceans without treatment or after treatment for some reason without reaching the GB8978-1669 Integrated Wastewater Discharge Standard, and eventually make them polluted. Because they cannot be biodegraded, on the contrary they can It is enriched under the biomagnification of the food chain and finally enters the human body. Heavy metals can st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
Inventor 王坤杰李明亮李红霞吴平关凤冯辉霞王毅张德懿
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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