Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof

A technology of bacterial cellulose and hydroxyapatite, applied in medical science, prostheses, etc., can solve the problems of difficult access to bacterial cellulose nanopores and uneven distribution of collagen, so as to facilitate growth and reproduction and improve biological Compatibility, effect of good biocompatibility

Inactive Publication Date: 2014-04-02
TIANJIN UNIV
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this regard, CN102600507A "a cell culture scaffold material and its preparation method" reported a method for preparing bacterial cellulose / collagen composite materials by impregnation, but because collagen belongs to biological macromolecules, it is difficult to enter the nanopores of bacterial cellulose , so the distribution of collagen in the bacterial cellulose / collagen composite material obtained by this method is very uneven, and only exists on the surface of bacterial cellulose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof
  • Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof
  • Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Prepare bacterial cellulose according to the following process:

[0022] First, dissolve 12.5g of glucose, 3.75g of peptone, 5.00g of yeast powder and 5.00g of disodium hydrogen phosphate in a beaker filled with 500ml of distilled water, adjust the pH to 4.5 with glacial acetic acid, and pour the liquid into the Erlenmeyer flask , sterilized at 115°C for 0.5h as a liquid medium.

[0023] Take a loop of Acetobacter xylinum with an inoculation loop, insert it into 100ml of the above-mentioned liquid medium, tie the bottle mouth tightly with gauze, place it in a culture shaker, and shake it at 30°C for 30 hours to obtain Acetobacter xylinum seed solution.

[0024] Inoculate the Acetobacter xylinum seed solution into the sterilized liquid medium according to the inoculum amount of not less than 6% by volume, oscillate fully to make the bacteria liquid uniform, and culture at a constant temperature of 30°C for 5 days to obtain the bacterial cellulose gel film . Wash with 1...

Embodiment 2

[0032] Dissolve 0.1g of collagen in 100ml of acetic acid solution with a concentration of 0.25M, put 2g of bacterial cellulose dry film into the acetic acid solution of the collagen, and then place it in an ultrasonic cleaner with a power of 500W for 10h to take out the bacterial fiber Put the plain film into 100ml of proanthocyanidin aqueous solution with a concentration of 4g / L, and after ultrasonic oscillation for 4h, place the gel film in 50ml of CaCl with a concentration of 0.2mol / L 2 solution, soaked for 2 days, during which CaCl was replaced once a day 2 solution to obtain a precalcified gel film. Then put the gel film into the pre-prepared simulated body fluid and soak it at constant temperature for 7 days to obtain the bacterial cellulose / collagen / hydroxyapatite composite material. Other experimental conditions are the same as in Experimental Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bacterial cellulose / collagen / hydroxyapatite composite material and a preparation method thereof. In the method, the capillary force of internal pores and an ultrasonic-assisted collagen molecule diffusion method are adopted, a bacterial cellulose dry film, collagen, procyanidine, CaCl2 and simulated body fluid are taken as raw materials, and the mass ratio of the bacterial cellulose dry film to the collagen to the procyanidine to CaCl2 is (10-200):(1-50):(4-6):(6-10); the collagen is uniformly distributed in the network structure of the bacterial cellulose in an irregular shape, and the obtained compound is prepared into the bacterial cellulose / collagen / hydroxyapatite composite material by adopting a biomimetic mineralization method. The composite material disclosed by the invention has superior mechanical property and a unique three-dimensional nano-network structure, is more similar to human skeletal tissues on the aspects of structure and component, and is expected to meet the application requirement of a skeletal tissue engineering stent material.

Description

technical field [0001] The invention relates to a bacterial cellulose / collagen / hydroxyapatite composite material and a preparation method thereof. Background technique [0002] As one of the four new materials in the current era, biomaterials are closely related to human health, so they have received more and more attention and research. As a kind of biomaterial, tissue engineering scaffold has become a hot spot in scientific research and clinical application. At present, the materials used in the research and application of tissue engineering scaffolds mainly include collagen, chitosan, polylactic acid, and alginate. Compared with these biological materials, bacterial cellulose has many unique properties, such as natural ultra-fine three-dimensional network structure, excellent mechanical properties, ultra-high water holding capacity, good biocompatibility, etc., so it has been widely used Used in wound dressings, artificial skin, artificial blood vessels, artificial corn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/48A61L27/46
Inventor 万怡灶张晶罗红林朱勇王哲人
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products