Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof
A technology of bacterial cellulose and hydroxyapatite, applied in medical science, prostheses, etc., can solve the problems of difficult access to bacterial cellulose nanopores and uneven distribution of collagen, so as to facilitate growth and reproduction and improve biological Compatibility, effect of good biocompatibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Prepare bacterial cellulose according to the following process:
[0022] First, dissolve 12.5g of glucose, 3.75g of peptone, 5.00g of yeast powder and 5.00g of disodium hydrogen phosphate in a beaker filled with 500ml of distilled water, adjust the pH to 4.5 with glacial acetic acid, and pour the liquid into the Erlenmeyer flask , sterilized at 115°C for 0.5h as a liquid medium.
[0023] Take a loop of Acetobacter xylinum with an inoculation loop, insert it into 100ml of the above-mentioned liquid medium, tie the bottle mouth tightly with gauze, place it in a culture shaker, and shake it at 30°C for 30 hours to obtain Acetobacter xylinum seed solution.
[0024] Inoculate the Acetobacter xylinum seed solution into the sterilized liquid medium according to the inoculum amount of not less than 6% by volume, oscillate fully to make the bacteria liquid uniform, and culture at a constant temperature of 30°C for 5 days to obtain the bacterial cellulose gel film . Wash with 1...
Embodiment 2
[0032] Dissolve 0.1g of collagen in 100ml of acetic acid solution with a concentration of 0.25M, put 2g of bacterial cellulose dry film into the acetic acid solution of the collagen, and then place it in an ultrasonic cleaner with a power of 500W for 10h to take out the bacterial fiber Put the plain film into 100ml of proanthocyanidin aqueous solution with a concentration of 4g / L, and after ultrasonic oscillation for 4h, place the gel film in 50ml of CaCl with a concentration of 0.2mol / L 2 solution, soaked for 2 days, during which CaCl was replaced once a day 2 solution to obtain a precalcified gel film. Then put the gel film into the pre-prepared simulated body fluid and soak it at constant temperature for 7 days to obtain the bacterial cellulose / collagen / hydroxyapatite composite material. Other experimental conditions are the same as in Experimental Example 1.
PUM
Property | Measurement | Unit |
---|---|---|
Concentration | aaaaa | aaaaa |
Concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com