Sea cucumber cultivation water quality repair agent capable of effectively reducing water turbidity, preparation method and use thereof

A technology of aquaculture water quality and restoration agent, applied in the direction of biological water/sewage treatment, water/sludge/sewage treatment, application, etc., can solve the problems of excessive chemical residues and antibiotic residues, high mortality of sea cucumbers, and pollution of aquaculture water, etc. Achieve suspension and good controllability of sinking and floating, reduce stress response, avoid acidification and odor bottom effect

Inactive Publication Date: 2014-04-02
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the current situation in the prior art, such as serious pollution of aquaculture water quality, frequent occurrence of diseases, high mortality rate of sea cucumbers, excessive heavy metal

Method used

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  • Sea cucumber cultivation water quality repair agent capable of effectively reducing water turbidity, preparation method and use thereof
  • Sea cucumber cultivation water quality repair agent capable of effectively reducing water turbidity, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of probiotic stock solution

[0038] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix it at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×10 8 cfu / ml.

[0039] 2. Liquid fermentation culture

[0040] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0041] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0042] 3. Spray drying treatment

[0043] The composite ads...

Embodiment 2

[0046] 1. Preparation of probiotic stock solution

[0047] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix them at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0048] 2. Liquid fermentation culture

[0049] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0050] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0051] 3. Spray drying treatment

[0052] The adsorption car...

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Abstract

The invention discloses a sea cucumber cultivation water quality repair agent capable of effectively reducing the water turbidity, a preparation method and use thereof, belonging to the technical field of aquatic product cultivation and marine microorganism. The repair agent disclosed by the invention aims at the physiology behaviors and cultivation characteristics of the sea cucumbers and uses the marine microorganism techniques. Bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117 are separated and screened from the sea cucumber cultivation environment, horn flour and soya bean meal are used as the main cultivation raw materials, and the bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117 are cultivated in the culture medium at 28-37 DEG C for 24-48 h, and then diatomaceous earth, sepiolite and rice hulls are used as absorption and suspension composite supporters to dry the fermentation products, so as to provide the sea cucumber cultivation water quality repair agent capable of effectively reducing the water turbidity. The repair agent has high tolerance for water environment and good floatability, suspension and ups and downs controllability, and can absorb harmful substances such as heavy metal ions such as Pb<2+>, Cr<6+>, Cd<2+>, Cu<2+> in the water, greatly reduce the content of the harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, avoid water acidification and bottom stinking-up, effectively inhibit pathogenic microorganism, and prevent the occurrence of diseases of the sea cucumber.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microbes, and in particular relates to a water quality restoration agent for effectively reducing water turbidity and sea cucumber culture, as well as its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory farming methods. With the moder...

Claims

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Application Information

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IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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