Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer

A technology of biological fertilizers, microorganisms, applied in the direction of microorganism-based methods, chemicals for biological control, microorganisms, etc., to achieve the effect of increasing yield and reducing morbidity

Inactive Publication Date: 2014-04-02
SHANDONG PUFANG BIOTECH
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the bio-fertilizer containing the above-mentioned microorganisms cannot effectively control the pathogenic bacteria of the crops while increasing the crop yield.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer
  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer
  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Screening of this microorganism

[0032] 1. Material

[0033] Test soil: From the same continuous-cropping cucumber planting greenhouse in Shouguang City, Shandong Province, the topsoil was removed and the rhizosphere soil of 3-15cm was collected, and each sample was about 100g. Number the soil samples separately, put them in bags, and bring them back to the laboratory for storage in a refrigerator at 4°C.

[0034] Test medium: PDA medium, Bengal red medium.

[0035] 2. Preparation of soil suspension

[0036] Put the soil sample in a sterilized mortar and grind it in a sterile operating table until the particles are uniform. Using the 10-fold dilution method, first weigh 1g of ground soil, put it in a triangular flask containing 99mL sterile water and shake it for 30 minutes to make a 10-2 soil dilution, then take out 1mL and add to 9mL sterile Fully shake in water to make 10-3 dilution, and so on, make 10-4 dilution for use.

[0037] 3. Plate inoculation

[0038] Usi...

Embodiment 2

[0047] Example 2. Inhibition of this microorganism on crop pathogens

[0048] Test pathogens: Cucumber transformed Fusarium oxysporum, Botrytis cinerea, Verticillium dahliae, Cucumber Fusarium wilt, Rhizoctonia solani (Among them, Fusarium oxysporum Cucumber transformed type can cause cucumber Fusarium wilt, Botrytis cinerea It can cause gray mold on leaves, Verticillium dahliae can cause verticillium wilt of crops, and Rhizoctonia solani can cause sheath blight).

[0049] Test medium: PDA medium, PDB medium, Bengal medium.

[0050] In the embodiment of the present invention, the microbial growth rate method is used to determine the antibacterial activity of the sterile fermentation filtrate of the microorganism.

[0051] The above-preserved microorganisms were inoculated into PDB medium, cultured at 25°C and 160r / min for 8 days, and then filtered with 0.45μm microporous membrane to obtain sterile fermentation filtrate. Mix the filtrate with the sterilized PDA medium cooled to 50-60℃...

Embodiment 3

[0056] Example 3 Preparation of the microbial spore suspension

[0057] The specific preparation is as follows:

[0058] Transfer the microbial strains stored in cold storage on a PDA medium plate (200g potato, 20g sucrose, 18g agar, 1000mL distilled water) for 3 generations; then use an inoculation loop to pick the edge hyphae of the third generation colony and transfer to PDB In the liquid medium (200g potato, 20g sucrose, 1000mL distilled water), cultured at 25℃~30℃, the optimum temperature is 28℃, 120rpm, for 10 days to obtain the fermentation broth of this microorganism. The obtained fermentation broth was filtered through a 100μm sieve to obtain a spore suspension with a viable spore concentration of 3.0 - 5.0×10 10 cfu / ml.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The embodiment of the invention provides a microbe, an application of the microbe in bio-fertilizer preparation, a bio-fertilizer and an application of the bio-fertilizer. Compared with the existing microbe for the bio-fertilizer and the existing bio-fertilizer in the prior art, the microbe provided by the invention is rhizopus stolonifer PF102, so that the microbe can be used for preparation of the bio-fertilizer; when the bio-fertilizer used for crop cultivation, the bio-fertilizer can improve the output of the crops, greatly reduce the morbidity of the crops and death rate due to disease, and in the meantime improve the growth conditions of the crops such as microbial population at the crop rhizosphere, soil bulk density, soil porosity, length and width of the plant leaf, and fruit quantity.

Description

Technical field [0001] The present invention relates to the field of biological fertilizers, in particular to microorganisms and their applications in preparing biological fertilizers, biological fertilizers and their applications. Background technique [0002] In recent years, with the strengthening of people's awareness of environmental protection and the improvement of the quality of life, biological fertilizers are a green and efficient fertilizer because they will not pollute the environment. It has been rapidly developed and widely used in crop cultivation. [0003] In related technologies, the commonly used microorganisms in biological fertilizers include nitrogen-fixing bacteria: such as Rhizobium; phosphorus solubilizing bacteria: such as Bacillus megaterium, Bacillus licheniformis, Bacillus cereus, and Bacillus subtilis; potassium-resolving bacteria: such as Bacillus colloidi. [0004] However, biological fertilizers containing the above-mentioned microorganisms cannot effe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00A01P1/00A01P3/00A01P21/00C12R1/845
Inventor 杨文慧
Owner SHANDONG PUFANG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com