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Coarse cereal, mixed meal and daily ration enzyme preparation and preparation method thereof

A technology of miscellaneous grains and miscellaneous grains, which is applied in the field of miscellaneous grains and miscellaneous grains dietary enzyme preparations and its production, can solve the problems of low enzyme activity and poor thermal stability of β-glucanase, so as to promote animal growth, improve utilization rate, The effect of natural antibacterial immunity

Active Publication Date: 2014-04-30
SHANXI DAYU BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of enzyme preparations such as β-glucanase can effectively solve this problem, but most of the β-glucanases currently used in production and experiments have low enzyme activity and poor thermal stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of embodiment 1 Chinese herbal medicine powder

[0040] A preparation method of Chinese herbal medicine powder is as follows:

[0041] Weigh 25 parts of Radix Astragali; 15 parts of Codonopsis; 12 parts of Bupleurum; Keep at ℃ for 3h, then cool down to 50℃, add mixed enzyme preparation for enzymolysis, use lactic acid to adjust the pH value to 6, enzymolysis for 3h, finally add a mixture of ethanol and propanol twice the weight of the mixed material, 11W ultrasonic extraction for 1h, filter ; The filtrate was concentrated in vacuum and then freeze-dried to obtain Chinese herbal medicine powder.

[0042] The added amount of the mixed enzyme is 8% of the total weight of the mixed material.

[0043] The weight part array of the mixed enzyme is: 15 parts of internal β-glucanase, 15 parts of external β-glucanase, 12 parts of β-glucosidase, 18 parts of xylanase, 18 parts of pentosanase 18 parts, 18 parts of β-amylase, 12 parts of pectinase, 12 parts ...

Embodiment 2

[0045] Embodiment 2 A kind of preparation method of bacillus licheniformis culture

[0046] Including the following steps:

[0047] (1) Expanded cultivation of liquid seeds

[0048] ①First-level seed culture: put the activated slant strains into 500 ml shake flasks with 2 rings, the medium capacity is 100 ml, the rotary shaker is 180 rpm, the culture temperature is 40°C, and the culture time is 10 hours;

[0049] ②Cultivation of secondary seeds: Inoculate 10% of primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as primary seeds;

[0050] ③Third-level seed cultivation: Inoculate the second-level seeds with 10% inoculum amount into a 5000 ml third-level seed shaker flask, the medium capacity is 1000 ml, the rotary shaker is 100 rpm, the culture temperature is 40°C, and the culture time is 12 hours ;

[0051] ④First-level seed tank cultivation: Put the third-level seeds into the first-level seed tank with a total volume of 150L ...

Embodiment 3

[0059] Embodiment 3 A kind of preparation method of compound enzyme preparation for feed

[0060] A dietary enzyme preparation of miscellaneous grains and miscellaneous meal consists of the following enzyme preparations in parts by weight:

[0061] 1 part of Chinese herbal medicine powder, 110 parts of xylanase, 12 parts of protease, 12 parts of pectinase, 18 parts of cellulase, 35 parts of Bacillus licheniformis culture, 8 parts of mesophilic α-amylase.

[0062] According to the above formula, xylanase, protease, cellulase, medium-temperature α-amylase, pectinase, and the Chinese herbal medicine powder obtained in Example 1 obtained from Hunan Hongyingxiang Biological Engineering Co., Ltd. The above-mentioned Bacillus licheniformis culture was stirred evenly in a mixer at 25° C., and the stirring time was 5 minutes. Stir evenly and store in a box at room temperature or in a cold room.

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Abstract

The invention relates to a coarse cereal, mixed meal and daily ration enzyme preparation and a preparation method thereof, and belongs to the field of feed enzyme preparations. The coarse cereal, mixed meal and daily ration enzyme preparation consists of the following components in parts by weight: 0.5-2 parts of Chinese herbal medicine powder, 100-120 parts of xylanase, 10-15 parts of protease, 10-15 parts of pectinase, 15-20 parts of cellulase, 30-40 parts of bacillus licheniformis culture and 5-10 parts of intermediate temperature alpha-amylase, wherein the preservation number of the bacillus licheniformis beta-10-25 is CCTCC NO.M2013538. The preparation method comprises the following steps: fermenting the bacillus licheniformis, filtering, concentration and drying so as to obtain the culture, and compounding the culture with other components. The prepared composite enzyme is high in feed conversion rate, the feed cost is lowered, and the stability of feed enzyme is improved.

Description

technical field [0001] The invention belongs to the field of enzyme preparations, and in particular relates to a dietary enzyme preparation of miscellaneous grains and dregs and a manufacturing method thereof. Background technique [0002] With the development of modern enzyme technology and biotechnology, high-efficiency biologically active substances—enzyme preparations have been able to be industrially produced on a large scale and used in the feed industry. Many experiments and practical application results have shown that feed enzyme preparations are used as A feed additive can effectively improve feed utilization, promote animal growth and prevent animal diseases. Compared with antibiotics and hormones, it has excellent safety and has attracted great attention from the feed industry worldwide. [0003] Feed enzyme preparations are enzymes added to feed to improve animal digestion and utilization of feed or to improve metabolic performance in animals. Enzyme preparatio...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K1/14
Inventor 李洪兵李海清张锦杰朱永明胡永明
Owner SHANXI DAYU BIOLOGICAL ENG CO LTD
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