A kind of yeast glutathione nutritional preparation
A technology for nutritional preparations and yeast nutrition, applied in medical preparations containing active ingredients, tripeptide ingredients, food preparation, etc., can solve problems such as increased liver metabolism and detoxification burden, decreased antioxidant capacity, tissue damage, etc. It is beneficial to human body utilization, eliminates toxins from the body, and has low environmental requirements.
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Embodiment 1
[0022] Embodiment 1 is rich in the preparation of the Saccharomyces cerevisiae fermented liquid that is rich in GSH
[0023] Saccharomyces cerevisiae1251 was purchased from: China Industrial Microbiology Culture Collection Management Center
[0024] Insert strain Saccharomyces cerevisiae into plate culture medium BMGY, culture temperature 26-30°C, after 6-8 days, then move to slant medium BMGY, 26-30°C, 6-8 days to obtain slant spores.
[0025]Use a sterile inoculation loop to dig out 0.5-1cm 2 The slant spores of the first-level seed medium BMGY were cultivated at 26-30°C for 20-30h, and then the first-level seeds were inoculated into the next-level seed medium BMGY, and cultivated at 26-30°C for 20-30h. The inoculation amount of each level of seeds is 10%.
[0026] Insert the last level of seeds into the fermentation medium with an inoculum size of 10%. Medium contains: glucose 25g / l, yeast powder 15g / l, wort 60g / l, ammonium phosphate 10g / l, magnesium sulfate 5g / l, dipota...
Embodiment 2
[0028] Embodiment 2 is rich in the preparation of the Saccharomyces cerevisiae fermented liquid that is rich in GSH
[0029] Saccharomyces cerevisiae1210 was purchased from: China Industrial Microorganism Culture Collection Management Center
[0030] Medium contains: glucose 25g / l, yeast powder 15g / l, wort 60g / l, ammonium phosphate 10g / l, magnesium sulfate 5g / l, dipotassium hydrogen phosphate 1g / l, potassium dihydrogen phosphate 1g / l, chlorine Calcium 10mg / l, sodium chloride 1mg / l. The culture temperature is 26-28°C, the tank pressure is 0-0.05MPa, the air volume is 1:1-1:2 by volume, the pH is controlled at 6.0 during the fermentation process, glycerin is added after the fermentation starts, and the glycerin concentration is 100g / L. The total glycerol content is 2.0g / 100ml, add inorganic selenium ions, the addition amount is 20mg / L, add 8mmol / l L-cysteine, L-glutamic acid, glycine and 4mmol / l adenosine triphosphate after 1 day of fermentation, Ferment for 96h and collect th...
Embodiment 3
[0031] Embodiment 3 is rich in the preparation of the Saccharomyces cerevisiae fermented liquid that is rich in GSH
[0032] Saccharomyces cerevisiae1337 was purchased from: China Industrial Microorganism Culture Collection Management Center. Bacteria were irradiated with 10W UV lamp at 25cm for 3min, turned off the UV lamp, and placed in the dark for 5min. The obtained mutagen has higher GSH production capacity. Medium contains: glucose 25g / l, yeast powder 15g / l, wort 60g / l, ammonium phosphate 10g / l, magnesium sulfate 5g / l, dipotassium hydrogen phosphate 1g / l, potassium dihydrogen phosphate 1g / l, chlorine Calcium 10mg / l, sodium chloride 1mg / l. The culture temperature is 26-28°C, the tank pressure is 0-0.05MPa, the air volume is 1:1-1:2 by volume, the pH is controlled at 6.0 during the fermentation process, glycerin is added after the fermentation starts, and the glycerin concentration is 100g / L. The total glycerol content is 2.0g / 100ml, add inorganic selenium ions, the add...
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